| Definition | Escherichia coli HS, complete genome. |
|---|---|
| Accession | NC_009800 |
| Length | 4,643,538 |
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The map label for this gene is nagE [H]
Identifier: 157160157
GI number: 157160157
Start: 734233
End: 736179
Strand: Direct
Name: nagE [H]
Synonym: EcHS_A0723
Alternate gene names: 157160157
Gene position: 734233-736179 (Clockwise)
Preceding gene: 157160150
Following gene: 157160159
Centisome position: 15.81
GC content: 54.6
Gene sequence:
>1947_bases ATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTT GCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCAT TAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCAGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTAC TTTGTGTTAACCAAAGCGATGGTGACCATCAACCCAGAAATAAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGT TGGTGGCGCAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGC CGATTGCCACCGGCTTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCTGGCCGCCGGTACAGCACGCTATCCAT GCAGGAGGCGAGTGGATCGTTTCTGCGGGCGCGCTGGGTTCCGGTATCTTTGGTTTCATCAACCGTCTGCTGATCCCAAC CGGTCTGCATCAGGTGCTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACG GTGATATCAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATTATGATGTTCGGT CTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGGATGCTGCTTTCTGT TGCTGTTACTGCGTTCCTGACCGGTGTGACTGAGCCGCTGGAATTCCTGTTCATGTTCCTTGCACCGCTGCTGTACCTCC TGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACACTGCTGGGTATCCATGCTGGCTTCTCTTTCTCTGCGGGG GCTATCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGTGTTGTCTT CTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG ACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGCCTCAATCAACTGGCGACCAACTATATTGCTGCGGTT GGCGGCACTGACAACCTGAAAGCAATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAA CGATACGATGTGTAAACGTCTGGGTGCTTCTGGTGTAGTAAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGA AAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGCGGTCCGGTAGCAGCTGCGTCAGCTGAAGCAACTCCG GCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGG TGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGA CAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACC GAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGA AGAGGGTGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGATTACCTGAACGCTAACGCCCGCTCGATGA TTAGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATGTTGTGGCGGGTCAA ACACCGCTGTATGAAATCAAAAAGTAA
Upstream 100 bases:
>100_bases GATCTAACAATCTGATTACAGAACATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTTAAACGAGCCAAATAGG GTTCTCGTAGGGGGAATAAG
Downstream 100 bases:
>100_bases TCTGCTTTATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAAAGCTCAGTCCAGGTTGGATAAGACGCTTAA GCGTCGCATCCAACAATGAC
Product: N-acetyl glucosamine specific PTS system components IIABC
Products: NA
Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]
Number of amino acids: Translated: 648; Mature: 648
Protein sequence:
>648_residues MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV GGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ TPLYEIKK
Sequences:
>Translated_648_residues MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV GGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ TPLYEIKK >Mature_648_residues MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV GGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ TPLYEIKK
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1786894, Length=648, Percent_Identity=99.537037037037, Blast_Score=1298, Evalue=0.0, Organism=Escherichia coli, GI1787343, Length=485, Percent_Identity=40.8247422680412, Blast_Score=367, Evalue=1e-102, Organism=Escherichia coli, GI1787908, Length=511, Percent_Identity=34.2465753424658, Blast_Score=258, Evalue=6e-70, Organism=Escherichia coli, GI1788757, Length=149, Percent_Identity=50.3355704697987, Blast_Score=158, Evalue=1e-39, Organism=Escherichia coli, GI1790159, Length=174, Percent_Identity=36.2068965517241, Blast_Score=113, Evalue=4e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011055 - InterPro: IPR018113 - InterPro: IPR004719 - InterPro: IPR001127 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 - InterPro: IPR011535 - InterPro: IPR010974 [H]
Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 68333; Mature: 68333
Theoretical pI: Translated: 6.06; Mature: 6.06
Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCC PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCCCCCHHHHHH NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIR HHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH MFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTV HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEE ADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP CCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHH MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQTPLYEIKK HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHCCC >Mature Secondary Structure MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCC PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCCCCCHHHHHH NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIR HHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH MFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTV HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEE ADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP CCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHH MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQTPLYEIKK HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3284790; 3056518; 8905232; 9278503 [H]