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The map label for this gene is ubiF

Identifier: 157160150

GI number: 157160150

Start: 724111

End: 725286

Strand: Direct

Name: ubiF

Synonym: EcHS_A0709

Alternate gene names: 157160150

Gene position: 724111-725286 (Clockwise)

Preceding gene: 157160144

Following gene: 157160157

Centisome position: 15.59

GC content: 57.14

Gene sequence:

>1176_bases
ATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCA
CGGATTTGCGGTAACGGTGATCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGG
CGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGCGTTGCCATCCTTAC
CGCAGACTGGAAACGTGGGAGTGGGAAACGGCGCATGTGGTGTTTGACGCCGCTGAACTTAAGCTACCGCTGCTTGGCTA
TATGGTGGAAAACACTGTCCTGCAACAGGCGTTGTGGCAGGCGCTGGAAGCGCATCCGAAAGTAACGTTACGTGTGCCAG
GCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTG
GTGATTGGTGCCGACGGCGCAAATTCGCAGGTGCGGCAGATGGCGGGAATTGGCGTTCATGCATGGCAGTATGCGCAGTC
GTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTG
CGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATG
AATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGC
GTTTCCGCTGACGCGTCGCCATGCGTTGCAGTACGTGCAGCCAGGGCTTGCGCTGGTGGGCGATGCCGCGCATACCATCC
ATCCGCTGGCGGGGCAGGGAGTGAATCTTGGTTATCGTGATGTCGATGCCCTGATTGATGTTCTGGTCAACGCCCGCAGC
TACGGCGAAGCGTGGGCCAGTTATCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAG
CGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGG
CGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAG

Upstream 100 bases:

>100_bases
TGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATAT
TGCTAACAGGATGATTGACC

Downstream 100 bases:

>100_bases
CCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGA
CGTGTCAACATCGCATTCGA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPY
RRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARS
YGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL

Sequences:

>Translated_391_residues
MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPY
RRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARS
YGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL
>Mature_390_residues
TNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYR
RLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKLV
IGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNMN
MAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSY
GEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family

Homologues:

Organism=Homo sapiens, GI32967305, Length=390, Percent_Identity=32.3076923076923, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI32967307, Length=349, Percent_Identity=31.8051575931232, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=100, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=38.7755102040816, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=32.258064516129, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=436, Percent_Identity=27.7522935779816, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321694, Length=451, Percent_Identity=24.8337028824834, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19920760, Length=419, Percent_Identity=27.6849642004773, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIF_ECOLI (P75728)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U82598
- PIR:   D64801
- RefSeq:   AP_001311.1
- RefSeq:   NP_415195.1
- ProteinModelPortal:   P75728
- DIP:   DIP-11069N
- IntAct:   P75728
- STRING:   P75728
- PRIDE:   P75728
- EnsemblBacteria:   EBESCT00000001793
- EnsemblBacteria:   EBESCT00000015553
- GeneID:   945261
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0659
- KEGG:   eco:b0662
- EchoBASE:   EB3422
- EcoGene:   EG13658
- eggNOG:   COG0654
- GeneTree:   EBGT00050000008989
- HOGENOM:   HBG464401
- OMA:   AYDLRIS
- ProtClustDB:   PRK08020
- BioCyc:   EcoCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON
- BioCyc:   MetaCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON
- Genevestigator:   P75728
- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- PRINTS:   PR00420
- TIGRFAMs:   TIGR01988

Pfam domain/function: PF01494 FAD_binding_3

EC number: 1.14.13.-

Molecular weight: Translated: 42954; Mature: 42823

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASV
CCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ
HHHHHCCHHHHHHHHHCCCCHHCCCCCCCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHH
ALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH
HHHCCCCEEEECCCCEEEEECCCCHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
HHHHHCCEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHHCCCEEEEEECCHHHHHHHHCC
NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL
CCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRFMRNLGLMAAERAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASV
CCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ
HHHHHCCHHHHHHHHHCCCCHHCCCCCCCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHH
ALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH
HHHCCCCEEEECCCCEEEEECCCCHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCCCHH
AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
HHHHHCCEEEEEEEECCCCCCCHHHHCCCCCCEEEHHHHCCCEEEEEECCHHHHHHHHCC
NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
CHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL
CCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PPLRFMRNLGLMAAERAGVLKRQALKYALGL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164