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The map label for this gene is entB

Identifier: 157160091

GI number: 157160091

Start: 663775

End: 664632

Strand: Direct

Name: entB

Synonym: EcHS_A0646

Alternate gene names: 157160091

Gene position: 663775-664632 (Clockwise)

Preceding gene: 157160090

Following gene: 157160092

Centisome position: 14.29

GC content: 55.36

Gene sequence:

>858_bases
ATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTGACTGGGCCTTTGA
ACCGCAACGTGCCGCGTTGTTAATCCATGATATGCAGGACTATTTTGTCAGCTTCTGGGGCGAGAACTGCCCGATGATGG
AGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCG
AAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCGGGCCTGACCCGCTCGCCGGAACAGCAAAA
GGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGC
TGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACC
GCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCGCTGGCCGATTTCAGCCGTGACGAGCATTT
GATGTCGCTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCA
GCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGAC
TACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCT
GGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAA

Upstream 100 bases:

>100_bases
TGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCGCATC
AGCCTGAAGGAGAGAACACG

Downstream 100 bases:

>100_bases
TGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAG
GCGGGAGCGAAAGTTACAGG

Product: isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLID
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK

Sequences:

>Translated_285_residues
MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLID
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
>Mature_284_residues
AIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPK
EQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTA
TDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLIDY
GLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain

Homologues:

Organism=Escherichia coli, GI1786811, Length=285, Percent_Identity=100, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENTB_ECO57 (P0ADI5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90708
- PIR:   F85558
- RefSeq:   NP_286322.1
- RefSeq:   NP_308661.1
- ProteinModelPortal:   P0ADI5
- SMR:   P0ADI5
- EnsemblBacteria:   EBESCT00000028290
- EnsemblBacteria:   EBESCT00000059955
- GeneID:   916993
- GeneID:   957609
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0737
- KEGG:   ecs:ECs0634
- GeneTree:   EBGT00050000011093
- HOGENOM:   HBG289118
- OMA:   YASNRCA
- ProtClustDB:   CLSK879705
- BioCyc:   ECOL83334:ECS0634-MONOMER
- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163
- Gene3D:   G3DSA:1.10.1200.10
- Gene3D:   G3DSA:3.40.50.850
- PIRSF:   PIRSF001111
- PRINTS:   PR01398

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding; SSF52499 Iscrsm_hydrolase

EC number: =3.3.2.1

Molecular weight: Translated: 32555; Mature: 32423

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50075 ACP_DOMAIN; PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI
CCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEE
LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD
EEEEEHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLID
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEE
LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD
EEEEEHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLID
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796