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The map label for this gene is dnaX [H]

Identifier: 157159997

GI number: 157159997

Start: 558157

End: 560088

Strand: Direct

Name: dnaX [H]

Synonym: EcHS_A0547

Alternate gene names: 157159997

Gene position: 558157-560088 (Clockwise)

Preceding gene: 157159996

Following gene: 157159998

Centisome position: 12.02

GC content: 57.56

Gene sequence:

>1932_bases
ATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGC
ACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTA
TCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGT
GAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCCTCGCGCACCAAAGTTGAAGATACCCGCGACCT
GCTGGATAACGTCCAGTACGCTCCGGCGCGTGGTCGTTTCAAAGTTTATCTGATCGACGAAGTGCATATGCTGTCGCGCC
ACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAG
AAATTGCCGGTGACGATTTTGTCACGCTGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCT
TGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGTTGCAATTGCTGGCGCGCGCCGCTGAAGGCAGCC
TGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCAGGTTTCAACCCAGGCGGTCAGTGCGATG
CTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCT
GATTAATGAAGCCGCTGCCCGTGGTATCGAGTGGGAAGCGTTGCTGGTGGAAATGCTCGGTCTGTTGCATCGTATTGCGA
TGGTACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATA
CCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGCCGCAT
GGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGCCAGT
CCTTTGCACCTGTCGCACCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCT
CCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAA
AGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCA
CCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCTAAAAAAGAAGCGTATCGCTGGAAGGCG
ACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCC
GGAACTGGCGGCGAAGCTGGCGGCAGAAGCCATTGAGCGCGACGCGTGGGCGGCACAGGTTAGCCAACTTTCGCTACCAA
AACTGGTCGAACAGGTGGCCTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCACCTCTCAG
CGGCATTTGAACAACCGCGGTGCACAGCAAAAACTGGCTGAAGCGTTGAGCACGTTAAAAGGTTCAACGGTTGAACTGAC
TATCGTTGAAGATGATAATCCCGCGGTGCGTACGCCGCTGGAATGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGG
CGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGATTCTTCGATGCGGAGCTGGATGAAGACAGTATC
CGCCCCATTTGA

Upstream 100 bases:

>100_bases
ACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTC
CAGCGTTTCAGAGCCTGCCA

Downstream 100 bases:

>100_bases
TCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACC
TGATGAAGCAAGCCCAGCAG

Product: DNA polymerase III subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCR
EIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTI
PPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQA
PTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRTSQ
RHLNNRGAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEDSI
RPI

Sequences:

>Translated_643_residues
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCR
EIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTI
PPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQA
PTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRTSQ
RHLNNRGAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEDSI
RPI
>Mature_642_residues
SYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCRE
IEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQK
LPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAML
GTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIP
PTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAP
TVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKAT
TPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRTSQR
HLNNRGAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEDSIR
PI

Specific function: The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI6677723, Length=311, Percent_Identity=24.7588424437299, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI194306571, Length=314, Percent_Identity=24.5222929936306, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194306567, Length=314, Percent_Identity=24.5222929936306, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786676, Length=643, Percent_Identity=99.3779160186625, Blast_Score=1297, Evalue=0.0,
Organism=Escherichia coli, GI1787341, Length=139, Percent_Identity=31.6546762589928, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324039, Length=227, Percent_Identity=25.5506607929515, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322528, Length=278, Percent_Identity=24.1007194244604, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18859927, Length=275, Percent_Identity=24.7272727272727, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR001270
- InterPro:   IPR008921
- InterPro:   IPR022001
- InterPro:   IPR022754
- InterPro:   IPR012763
- InterPro:   IPR021029 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; PF12168 DNA_pol3_tau_4; PF12170 DNA_pol3_tau_5 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 71082; Mature: 70951

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAK
CCHHHHHHHCCCCHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDV
EEEEECHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCH
EQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
HHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPA
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHH
ALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFH
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
PRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQ
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
HHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCC
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDAWAAQVSQLSLPKLVEQVA
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNAWKEESDNAVCLHLRTSQRHLNNRGAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPL
HHHHHCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCH
EWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEDSIRPI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAK
CHHHHHHHCCCCHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDV
EEEEECHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCH
EQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
HHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPA
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHH
ALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFH
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
PRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQ
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
HHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCC
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDAWAAQVSQLSLPKLVEQVA
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNAWKEESDNAVCLHLRTSQRHLNNRGAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPL
HHHHHCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCH
EWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEDSIRPI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534795; 3018672; 9278503; 2186364; 2187190; 2181440; 1575709 [H]