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The map label for this gene is apt [H]

Identifier: 157159996

GI number: 157159996

Start: 557477

End: 558028

Strand: Direct

Name: apt [H]

Synonym: EcHS_A0546

Alternate gene names: 157159996

Gene position: 557477-558028 (Clockwise)

Preceding gene: 157159995

Following gene: 157159997

Centisome position: 12.01

GC content: 53.99

Gene sequence:

>552_bases
ATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCT
TTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAA
ATGCGGGCATTACCAAAGTTGTCGGCACCGAAGCGCGTGGCTTCTTGTTTGGCGCTCCGGTAGCTCTGGGTCTGGGCGTT
GGCTTTGTGCCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACTATCAGTGAAACTTACGATCTGGAATACGGCACCGA
TCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGATCTGCTGGCAACCGGCGGCA
CTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAT
CTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCCGGGCCATTAA

Upstream 100 bases:

>100_bases
TTTCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCG
TAACAAGCAGGCATACACTT

Downstream 100 bases:

>100_bases
TTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTT
AGCATTACCCCTTCGTGAAT

Product: adenine phosphoribosyltransferase

Products: NA

Alternate protein names: APRT [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGV
GFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
LGGEQRLEKQGITSYSLVPFPGH

Sequences:

>Translated_183_residues
MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGV
GFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
LGGEQRLEKQGITSYSLVPFPGH
>Mature_182_residues
TATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVG
FVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFDL
GGEQRLEKQGITSYSLVPFPGH

Specific function: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis [H]

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4502171, Length=164, Percent_Identity=43.2926829268293, Blast_Score=140, Evalue=5e-34,
Organism=Homo sapiens, GI71773201, Length=130, Percent_Identity=45.3846153846154, Blast_Score=118, Evalue=3e-27,
Organism=Escherichia coli, GI1786675, Length=183, Percent_Identity=100, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17509087, Length=166, Percent_Identity=43.3734939759036, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323619, Length=170, Percent_Identity=40, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320649, Length=171, Percent_Identity=36.2573099415205, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136334, Length=168, Percent_Identity=44.6428571428571, Blast_Score=139, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005764
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.7 [H]

Molecular weight: Translated: 19859; Mature: 19728

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKV
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEHHHHHHHHHHCCCCEEE
VGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGD
ECCCCCCEEEECHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCC
KVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFDLGGEQRLEKQGITSYSLVPF
EEEEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECC
PGH
CCC
>Mature Secondary Structure 
TATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALSIDLLVERYKNAGITKV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEHHHHHHHHHHCCCCEEE
VGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGD
ECCCCCCEEEECHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCC
KVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFDLGGEQRLEKQGITSYSLVPF
EEEEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECC
PGH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA