Click here to switch to the map view.

The map label for this gene is rfbD [H]

Identifier: 157158895

GI number: 157158895

Start: 2294001

End: 2294900

Strand: Reverse

Name: rfbD [H]

Synonym: EcE24377A_2331

Alternate gene names: 157158895

Gene position: 2294900-2294001 (Counterclockwise)

Preceding gene: 157154898

Following gene: 157157449

Centisome position: 46.09

GC content: 46.33

Gene sequence:

>900_bases
ATGAATATTCTCCTTTTCGGCAAAACAGGGCAGGTAGGTTGGGAACTACAGCGTGCTCTGGCACCTTTGGGTAATTTGAT
TGCTCTTGATGTTCACTCCACTGATTATTGTGGTGATTTTAGTAATCCTGAAGGTGTGGCTGAAACCGTCAAAAAAATTC
GCCCTGATGTTATTGTTAATGCTGCGGCTCATACCGCAGTAGATAAGGCTGAGTCAGAACCCGAATTTGCACAATTACTC
AATGCGACCAGCGTTGAATCAATTGCAAAAGCGGCTAATGAAGTTGGGGCCTGGGTAATTCATTACTCAACTGACTACGT
ATTTCCGGGAACCGGTGAAATACCATGGCTGGAGACGGATGCAACCGCACCGCTAAATGTTTACGGTGAAACCAAGTTAG
CCGGAGAAAAAGCGTTACAGGAACATTGCGCGAAGCATCTTATTTTCCGTACCAGCTGGGTATACGCAGGTAAAGGAAAT
AACTTCGCCAAAACGATGTTGCGTCTGGCAAAAGAGCGTGAAGAATTAGCGGTTATTAACGATCAGTTTGGTGCACCAAC
GGGTGCTGAGCTGCTGGCTGATTGTACGGCACATGCTATTCGTGTTGCACTGAAAAAACCAGAAGTCGCAGGCTTGTACC
ATTTGGTAGCCAGTGGTACCACAACCTGGTACGATTATGCTGCACTGGTTTTTGAAGAGGCGCGCAAAGCAGGCATTCCC
CTTGCACTCAACAAGTTCAACGCAGTACCAACAACTGCCTATCCTACACCAGCTCGTCGTCCACATAACTCTCGCCTTAA
TACAGAAAAATTTCAGCAGAACTTTGCGCTTGTCTTGCCTGACTGGCAGGTTGGCGTGAAACGAATGCTCAACGAATTAT
TTACGACTACAGCAATTTAA

Upstream 100 bases:

>100_bases
AGACAGTGGAATGGTACCTGTCCAATACAAAATGGGTTGATAATGTGAAAAGTGGTGCCTATCAATCGTGGATTGAACAG
AACTATGAGGGCCGCCAGTA

Downstream 100 bases:

>100_bases
TAGTTTTTGCATCTTGTTCGTGATGGTGGAGCAAGATGAATTAAAAGGAATGATGAAATGAAAACGCGTAAAGGTATTAT
TTTAGCGGGTGGTTCTGGTA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEGVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLL
NATSVESIAKAANEVGAWVIHYSTDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
LALNKFNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI

Sequences:

>Translated_299_residues
MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEGVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLL
NATSVESIAKAANEVGAWVIHYSTDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
LALNKFNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI
>Mature_299_residues
MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEGVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLL
NATSVESIAKAANEVGAWVIHYSTDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
LALNKFNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=251, Percent_Identity=27.8884462151394, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI33519455, Length=251, Percent_Identity=27.8884462151394, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=95.6521739130435, Blast_Score=598, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32694; Mature: 32694

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEGVAETVKKIRPDVIVN
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEE
AAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHYSTDYVFPGTGEIPWLETD
HHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHCEEEEEEECCEEECCCCCCCEEECC
ATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGNNFAKTMLRLAKEREELAVIN
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHEEEC
DQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
LALNKFNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEGVAETVKKIRPDVIVN
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEE
AAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHYSTDYVFPGTGEIPWLETD
HHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHCEEEEEEECCEEECCCCCCCEEECC
ATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGNNFAKTMLRLAKEREELAVIN
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHEEEC
DQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
LALNKFNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559340 [H]