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The map label for this gene is rfbB2 [H]

Identifier: 157154898

GI number: 157154898

Start: 2294900

End: 2295985

Strand: Reverse

Name: rfbB2 [H]

Synonym: EcE24377A_2332

Alternate gene names: 157154898

Gene position: 2295985-2294900 (Counterclockwise)

Preceding gene: 157159205

Following gene: 157158895

Centisome position: 46.11

GC content: 43.37

Gene sequence:

>1086_bases
GTGAAGATACTTGTTACTGGTGGCGCAGGATTTATTGGTTCTGCTGTAGTTCGTCACATTATAAATAATACGCAGGATAG
TGTTGTTAATGTCGATAAATTAACGTACGCCGGAAACCTGGAATCACTTGCTGATGTTTCTGATTCCGAACGCTATGTTT
TTGAACATGCGGATATTTGCGATGCAGCTGTAATGGCACGGATTTTTGCTCAGCATCAGCCGGATGCAGTGATGCACCTG
GCAGCTGAAAGCCATGTTGACCGTTCAATTACAGGCCCTGCGGCATTTATTGAAACCAATATTGTTGGTACTTATGTCCT
TTTAGAGGCCGCTCGGAATTACTGGTCTGCTCTTGATGGCGACAAGAAAAATAGTTTCCGTTTTCATCATATTTCTACTG
ACGAAGTCTATGGTGATTTGCCTCATCCAGATGAAGTAAATAATACAGAAGAATTACCCTTATTTACTGAGACGACAGCT
TACGCGCCAAGCAGCCCTTATTCCGCATCCAAAGCATCCAGCGATCATTTAGTCCGTGCGTGGAAACGTACCTATGGTTT
ACCGACCATTGTGACTAATTGTTCGAATAACTACGGTCCTTATCACTTTCCGGAAAAATTGATTCCACTAGTAATTCTTA
ATGCTCTGGAAGGTAAGGCATTACCTATTTATGGCAAAGGGGATCAAATTCGCGACTGGCTGTATGTTGAAGATCATGCA
CGTGCGTTATATACCGTCGTAACCGAAGGTAAAGCGGGTGAAACTTATAACATTGGTGGACACAACGAAAAGAAAAACAT
CGATGTAGTGCTCACTATTTGTGATTTGTTGGATGAGATTGTACCGAAAGAGAAATCTTACCGCGAGCAAATTACTTATG
TTGCCGATCGTCCGGGACACGATCGCCGTTATGCCATTGATGCTGAGAAGATTGGTCGCGAATTGGGATGGAAACCACAG
GAAACGTTTGAGAGCGGGATTCGGAAGACAGTGGAATGGTACCTGTCCAATACAAAATGGGTTGATAATGTGAAAAGTGG
TGCCTATCAATCGTGGATTGAACAGAACTATGAGGGCCGCCAGTAA

Upstream 100 bases:

>100_bases
GCTGTAAAGCCAGGGGCGGTAGCGTGCATTAATACCTCTATTAATCAAACAAAGAGCCGCTTATTTCACAGCATGCTCTG
AAGTAATATGGAATAATAAA

Downstream 100 bases:

>100_bases
TGAATATTCTCCTTTTCGGCAAAACAGGGCAGGTAGGTTGGGAACTACAGCGTGCTCTGGCACCTTTGGGTAATTTGATT
GCTCTTGATGTTCACTCCAC

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ

Sequences:

>Translated_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ
>Mature_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQ
ETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGRQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=35.3115727002967, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=23.2558139534884, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=28.8184438040346, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=28.8184438040346, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=28.8184438040346, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4504031, Length=297, Percent_Identity=23.5690235690236, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=98.6149584487535, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=74.5042492917847, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=25.561797752809, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788366, Length=385, Percent_Identity=24.6753246753247, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=35.8208955223881, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=347, Percent_Identity=34.2939481268012, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=374, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=376, Percent_Identity=26.3297872340426, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=368, Percent_Identity=26.9021739130435, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=26.7029972752044, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=23.6686390532544, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24158427, Length=290, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40546; Mature: 40546

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCCEEEHHHHHH
DAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHEEECCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCCEEEHHHHHH
DAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALDG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSDHLVRA
CCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHEEECCCCC
DRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKWVDNVKSGAYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]