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The map label for this gene is otsB

Identifier: 157158032

GI number: 157158032

Start: 2108408

End: 2109208

Strand: Reverse

Name: otsB

Synonym: EcE24377A_2129

Alternate gene names: 157158032

Gene position: 2109208-2108408 (Counterclockwise)

Preceding gene: 157156388

Following gene: 157157530

Centisome position: 42.36

GC content: 50.56

Gene sequence:

>801_bases
GTGACAGAACCGTTAACCGAAACCCCTGAACTATCCGCGAAATATGCCTGGTTTTTTGATCTTGATGGAACGCTGGCGGA
AATCAAACCGCATCCCGATCAGGTCGTCGTGCCTGACAATATTCTGCAAGGACTACAGCTACTGGCAACCGCAAGTGATG
GTGCATTGGCATTGATATCAGGGCGCTCAATGGTGGAGCTTGACGCACTGGCAAAACCTTATCGCTTCCCGTTAGCGGGC
GTGCATGGGGCGGAGCGCCGTGACATCAATGGTAAAACACATATCGTTCATCTGCCGGATGCGATTGCGCGTGATATTAG
CGTGCAACTGCATACAGTCATCGCTCAGTATCCCGGCGCGGAGCTGGAGGCGAAAGGGATGGCTTTTGCGCTGCATTATC
GTCAGGCTCCGCAGCATGAAGACGCATTAATGACATTAGCGCAACGTATTACTCAGATCTGGCCACAAATGGCGTTACAG
CAGGGAAAGTGTGTTGTCGAGATCAAACCGAGAGGTACCAGTAAAGGTGAGGCAATTGCAGCTTTTATGCAGGAAGCTCC
CTTTATCGGGCGAACGCCCGTATTTCTGGGCGATGATTTAACCGATGAATCTGGCTTCGCAGTCGTTAACCGACTGGGCG
GAATGTCAGTAAAAATTGGCACAGGTGCAACTCAGGCATCATGGCGACTGGCGGGTGTGCCGGATGTCTGGAGCTGGCTT
GAAATGATAACCACCGCATTACAACAAAAAAGAGAAAATAACAGGAGTGATGACTATGAGTCGTTTAGTCGTAGTATCTA
A

Upstream 100 bases:

>100_bases
CTTCATTGCAAATGGCGACCCCCGTCACACTGTCTATACTTACATGTCTGTAAAGCGCGTTCTGCGCAAAACAATAAGAA
AAGAGAAGGAGGAGAGCCGG

Downstream 100 bases:

>100_bases
CCGGATTGCACCACCAGACGAGCACGCCGCCAGTGCCGGTGGCCTTGCCGTTGGCATACTGGGGGCACTGAAAGCCGCAG
GCGGACTGTGGTTTGGCTGG

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAG
VHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
EMITTALQQKRENNRSDDYESFSRSI

Sequences:

>Translated_266_residues
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAG
VHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
EMITTALQQKRENNRSDDYESFSRSI
>Mature_265_residues
TEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAGV
HGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQ
GKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWLE
MITTALQQKRENNRSDDYESFSRSI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family

Homologues:

Organism=Escherichia coli, GI1788207, Length=266, Percent_Identity=100, Blast_Score=548, Evalue=1e-157,
Organism=Drosophila melanogaster, GI19920676, Length=259, Percent_Identity=26.6409266409266, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221473368, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129307, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582490, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSB_ECOLI (P31678)

Other databases:

- EMBL:   X69160
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   I83401
- RefSeq:   AP_002516.1
- RefSeq:   NP_416411.1
- ProteinModelPortal:   P31678
- SMR:   P31678
- STRING:   P31678
- SWISS-2DPAGE:   P31678
- EnsemblBacteria:   EBESCT00000001034
- EnsemblBacteria:   EBESCT00000001035
- EnsemblBacteria:   EBESCT00000017436
- GeneID:   946406
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1886
- KEGG:   eco:b1897
- EchoBASE:   EB1702
- EcoGene:   EG11752
- eggNOG:   COG1877
- GeneTree:   EBGT00050000011447
- HOGENOM:   HBG686849
- OMA:   VGGHGAE
- ProtClustDB:   PRK10187
- BioCyc:   EcoCyc:TREHALOSEPHOSPHASYN-MONOMER
- BioCyc:   MetaCyc:TREHALOSEPHOSPHASYN-MONOMER
- Genevestigator:   P31678
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484
- TIGRFAMs:   TIGR00685

Pfam domain/function: PF02358 Trehalose_PPase; SSF56784 SSF56784

EC number: =3.1.3.12

Molecular weight: Translated: 29176; Mature: 29044

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALIS
CCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEC
GRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGA
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIA
CEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
HHHHHCCCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHH
EMITTALQQKRENNRSDDYESFSRSI
HHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure 
TEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALIS
CCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEC
GRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGA
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIA
CEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
HHHHHCCCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHH
EMITTALQQKRENNRSDDYESFSRSI
HHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503