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The map label for this gene is pduF [H]

Identifier: 157155813

GI number: 157155813

Start: 2245761

End: 2246570

Strand: Reverse

Name: pduF [H]

Synonym: EcE24377A_2278

Alternate gene names: 157155813

Gene position: 2246570-2245761 (Counterclockwise)

Preceding gene: 157156935

Following gene: 157155157

Centisome position: 45.12

GC content: 47.53

Gene sequence:

>810_bases
ATGAATGATTCACTCAAGGCGCAATGCGTTGCCGAGTTTTTAGGCACCGGACTGTTCCTCTTTTTTGGCATTGGTTGTTT
GTGCGCCCTGAAACTTGCTGGGGCAAGTCTGGGACTGTGGGAAATTTGTATCATTTGGGGGTTAGGGATTTCGCTTGCTG
TTTACCTTACGGCAGGTATTTCCGGCGCTCATCTCAACCCGGCTGTTACCATTGCTCTGTGGCTGTTTGCCTCTTTCCCA
GCCCGTAAAGTGCTGCCATTTTGTGTGGCGCAATTAGCGGGTGCCTTTGGTGGTGCGGTTCTGGCATATTCTCTATACAG
TAGTCTGTTTACCGATTTTGAATCTGCTCACAATATGGTGCGTGGTAGCGCAGAAAGTTTACAACTCGTCAGTATCTTCA
GTACTTATCCGGCAGCGGCGATTAATGTATGGCAAGCTGCGTTGGTCGAAGTGGTCATAACGTCCATGTTAATGGGGGTG
ATTATGGCGTTGACCGATGACGGTAATGGCGTACCCAAAGGACCGCTTGCACCTTTACTTATTGGTATTCTGGTTGCTGT
TATTGGTGCTTCTACCGGACCATTAACCGGTTTTGCCATGAATCCAGCGCGTGATTTTGGACCTAAGATTTTCACCTGGC
TTGCGGGGTGGGGAGAAATTGCGATGACTGGTGGACGCAATATTCCTTATTTCATTGTACCCATTATTGCACCGATCATT
GGGGCATGTGTTGGTGCGGCGATTTATCGCTATCTCATTGCAAAGAATTTACCTGTCAATAGTGTTACACCTAAAGGAAT
TAGCGAATAA

Upstream 100 bases:

>100_bases
ATATTTTCTGCAGAGCAATTTTTTATAGTTGCGCTCGGTATACACAACAATCAGAGCATAGTTGGCTGATAAAGAAAAAC
TCCGTAAAGAAGGTGTCACT

Downstream 100 bases:

>100_bases
CGAAATACTCTGGCAGTTATAAACATTTATTTTTTTACTGTTTGTGATCTGCTTTCCGAACTTATTAAAAAGTCTCCCGC
TTCACGGAGGCTTTTGTTAT

Product: propanediol diffusion facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFP
ARKVLPFCVAQLAGAFGGAVLAYSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGV
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWGEIAMTGGRNIPYFIVPIIAPII
GACVGAAIYRYLIAKNLPVNSVTPKGISE

Sequences:

>Translated_269_residues
MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFP
ARKVLPFCVAQLAGAFGGAVLAYSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGV
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWGEIAMTGGRNIPYFIVPIIAPII
GACVGAAIYRYLIAKNLPVNSVTPKGISE
>Mature_269_residues
MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFP
ARKVLPFCVAQLAGAFGGAVLAYSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGV
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWGEIAMTGGRNIPYFIVPIIAPII
GACVGAAIYRYLIAKNLPVNSVTPKGISE

Specific function: May facilitate the diffusion of propanediol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=261, Percent_Identity=36.0153256704981, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=35.15625, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI4502187, Length=261, Percent_Identity=34.4827586206897, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI4826645, Length=256, Percent_Identity=35.546875, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI310133356, Length=217, Percent_Identity=33.1797235023041, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310114181, Length=217, Percent_Identity=33.1797235023041, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI45446752, Length=203, Percent_Identity=33.9901477832512, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4502179, Length=262, Percent_Identity=32.0610687022901, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI6912506, Length=259, Percent_Identity=30.1158301158301, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4502183, Length=246, Percent_Identity=32.1138211382114, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI86792455, Length=248, Percent_Identity=31.4516129032258, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37694062, Length=250, Percent_Identity=28, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790362, Length=260, Percent_Identity=68.4615384615385, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787101, Length=220, Percent_Identity=30.9090909090909, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=254, Percent_Identity=34.6456692913386, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994009, Length=265, Percent_Identity=33.5849056603774, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564052, Length=258, Percent_Identity=32.5581395348837, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17533613, Length=258, Percent_Identity=32.5581395348837, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=37.3983739837398, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=262, Percent_Identity=29.7709923664122, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=262, Percent_Identity=29.7709923664122, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=254, Percent_Identity=31.496062992126, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558372, Length=266, Percent_Identity=28.1954887218045, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71993722, Length=241, Percent_Identity=28.6307053941909, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI212646268, Length=282, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=209, Percent_Identity=40.6698564593301, Blast_Score=139, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322985, Length=294, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550503, Length=261, Percent_Identity=26.8199233716475, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762344, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762346, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762348, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130305, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652747, Length=253, Percent_Identity=26.8774703557312, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551084, Length=253, Percent_Identity=26.8774703557312, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24762342, Length=243, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28031; Mature: 28031

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8071226; 9352910; 11677609 [H]