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The map label for this gene is vipA [H]

Identifier: 157155490

GI number: 157155490

Start: 2288314

End: 2289588

Strand: Reverse

Name: vipA [H]

Synonym: EcE24377A_2325

Alternate gene names: 157155490

Gene position: 2289588-2288314 (Counterclockwise)

Preceding gene: 157154752

Following gene: 157155425

Centisome position: 45.98

GC content: 34.59

Gene sequence:

>1275_bases
ATGAATATTGATAATATAAAAATAGGCATCATTGGTCTTGGTTATGTTGGCCTGCCTTTGGCGGTGGAATTCGGGAAAAA
ATATAATACTGTTGGTTTTGATATTAAACAAAACAGAATTGACGAATTAAAAAAACAAATGGATAGTACACTGGAGTGTA
CTGCGGATGAGCTTGCTAGTGCGAAATTTCTTCACTATTCATGTGAAATCGAAGCACTCAAAGCGTGTAATATATTTATT
GTTACTGTACCTACGCCAATTGATAAATATAAACGACCGGATTTAAGTCCCTTGGTTAGTGCTTCAAAAATGTTAAGCAA
GGTTATTAAAAATGGCGATATCATTATATTTGAATCGACGGTCTACCCAGGCGCAACAGAAGAAATATGTGTTCCTCAGA
TTGAAGCTGGTTCCGGATTAGTTTTCAACAAAGATTTTTTTATAGGCTATAGCCCAGAGAGAATCAATCCAGGAGATAAA
AATCATAGAGTAACAAATATTATGAAAGTAACTTCTGGTTCAACACCAGAAGCAGCAACGTTTGTGGATAAAATTTACTC
CTCAATTATAACAGCCGGGACATACAAGGCATCATCTATTAAGGTGGCTGAGGCCGCTAAGGTAATTGAAAATACGCAAC
GAGATCTTAATATTGCACTAATAAACGAATTGGCTATCATCTTCAATAAATTAGGTATTGATACTGAGGATGTTTTAAAG
GCAGCGGGTACTAAATGGAATTTTCTCCCTTTTCGTCCCGGCCTGGTTGGTGGTCATTGTATCGGGGTTGACCCATATTA
TTTAACGCATAAAGCTCAAGAAATTGGTTATCATCCAGAGGTTATTTTGGCTGGTAGACGTATTAATGATGATATGGCAC
GTTATGTTGCATCTCAACTTATAAAGGAAATGATTAAGGCAGGTATTCTAATAGATAATGCTAATATTTTAATTATGGGG
TTATCTTTTAAAGAAAATTGCCCTGATATTCGCAATACGAAAATTATTGATATAATATCAGAATTAAAAGATTATAATTT
GAATGTCGATGTTTATGACCCTTGGGTAAGTGAAGATGAGGCATTGCAGGAATATGGCATAGAAATTCATTCTACTAAAC
CTTCTAAGAAATATGAAGGCATAATTTTTGCTGTAGCCCATGAGCAATTCAAAAATATGCAATCCATTGAAATCAAGGAG
CTGCAGTCCACAAAATGTGTGACATATGATTTGAAATATATTCTTAATAAATCAGATGCAACAATTAGACTTTAA

Upstream 100 bases:

>100_bases
TTATTATAGTATTTGATATAATATCACCACTATTGTTTTATGTGTTTTAACAATTGGTTGTTTGTCTCTTATAAAATATG
AGTAAATAGTGAGAGTTTCT

Downstream 100 bases:

>100_bases
CTAGGTGAATATCTATGACTAGATATGAAAAAACTATTGAGCAATTAAAAGTTAAACCTAAGACTTGGTTGATAACAGGA
GTTGCTGGCTTCATTGGCTC

Product: VI polysaccharide biosynthesis protein VipA/tviB

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRIDELKKQMDSTLECTADELASAKFLHYSCEIEALKACNIFI
VTVPTPIDKYKRPDLSPLVSASKMLSKVIKNGDIIIFESTVYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERINPGDK
NHRVTNIMKVTSGSTPEAATFVDKIYSSIITAGTYKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTEDVLK
AAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGYHPEVILAGRRINDDMARYVASQLIKEMIKAGILIDNANILIMG
LSFKENCPDIRNTKIIDIISELKDYNLNVDVYDPWVSEDEALQEYGIEIHSTKPSKKYEGIIFAVAHEQFKNMQSIEIKE
LQSTKCVTYDLKYILNKSDATIRL

Sequences:

>Translated_424_residues
MNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRIDELKKQMDSTLECTADELASAKFLHYSCEIEALKACNIFI
VTVPTPIDKYKRPDLSPLVSASKMLSKVIKNGDIIIFESTVYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERINPGDK
NHRVTNIMKVTSGSTPEAATFVDKIYSSIITAGTYKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTEDVLK
AAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGYHPEVILAGRRINDDMARYVASQLIKEMIKAGILIDNANILIMG
LSFKENCPDIRNTKIIDIISELKDYNLNVDVYDPWVSEDEALQEYGIEIHSTKPSKKYEGIIFAVAHEQFKNMQSIEIKE
LQSTKCVTYDLKYILNKSDATIRL
>Mature_424_residues
MNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRIDELKKQMDSTLECTADELASAKFLHYSCEIEALKACNIFI
VTVPTPIDKYKRPDLSPLVSASKMLSKVIKNGDIIIFESTVYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERINPGDK
NHRVTNIMKVTSGSTPEAATFVDKIYSSIITAGTYKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTEDVLK
AAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGYHPEVILAGRRINDDMARYVASQLIKEMIKAGILIDNANILIMG
LSFKENCPDIRNTKIIDIISELKDYNLNVDVYDPWVSEDEALQEYGIEIHSTKPSKKYEGIIFAVAHEQFKNMQSIEIKE
LQSTKCVTYDLKYILNKSDATIRL

Specific function: Catalyzes The Oxidation Of Udp-Mannac To Udp-N- Acetylmannosaminuronic Acid (Udp-Mannaca). [C]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=357, Percent_Identity=29.1316526610644, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560350, Length=382, Percent_Identity=24.869109947644, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47249; Mature: 47249

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRIDELKKQMDSTLECTADELAS
CCCCEEEEEEEEECCCCCHHHEECCCCCCCCCCEECHHHHHHHHHHHCCHHHCCHHHHCC
AKFLHYSCEIEALKACNIFIVTVPTPIDKYKRPDLSPLVSASKMLSKVIKNGDIIIFEST
EEEEEEEECEEEEEECCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
VYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERINPGDKNHRVTNIMKVTSGSTPEAAT
CCCCCCCCCCCCEECCCCCEEEECCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHH
FVDKIYSSIITAGTYKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTEDVLK
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHH
AAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGYHPEVILAGRRINDDMARYVASQL
HCCCCCCEECCCCCCCCCEEECCCCHHEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHH
IKEMIKAGILIDNANILIMGLSFKENCPDIRNTKIIDIISELKDYNLNVDVYDPWVSEDE
HHHHHHCCEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHH
ALQEYGIEIHSTKPSKKYEGIIFAVAHEQFKNMQSIEIKELQSTKCVTYDLKYILNKSDA
HHHHHCCEEECCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHCCCEEEEEEEHEEECCCCC
TIRL
EEEC
>Mature Secondary Structure
MNIDNIKIGIIGLGYVGLPLAVEFGKKYNTVGFDIKQNRIDELKKQMDSTLECTADELAS
CCCCEEEEEEEEECCCCCHHHEECCCCCCCCCCEECHHHHHHHHHHHCCHHHCCHHHHCC
AKFLHYSCEIEALKACNIFIVTVPTPIDKYKRPDLSPLVSASKMLSKVIKNGDIIIFEST
EEEEEEEECEEEEEECCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
VYPGATEEICVPQIEAGSGLVFNKDFFIGYSPERINPGDKNHRVTNIMKVTSGSTPEAAT
CCCCCCCCCCCCEECCCCCEEEECCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHH
FVDKIYSSIITAGTYKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTEDVLK
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHH
AAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEIGYHPEVILAGRRINDDMARYVASQL
HCCCCCCEECCCCCCCCCEEECCCCHHEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHH
IKEMIKAGILIDNANILIMGLSFKENCPDIRNTKIIDIISELKDYNLNVDVYDPWVSEDE
HHHHHHCCEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHH
ALQEYGIEIHSTKPSKKYEGIIFAVAHEQFKNMQSIEIKELQSTKCVTYDLKYILNKSDA
HHHHHCCEEECCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHCCCEEEEEEEHEEECCCCC
TIRL
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]