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The map label for this gene is vipB [H]

Identifier: 157155425

GI number: 157155425

Start: 2287268

End: 2288299

Strand: Reverse

Name: vipB [H]

Synonym: EcE24377A_2324

Alternate gene names: 157155425

Gene position: 2288299-2287268 (Counterclockwise)

Preceding gene: 157155490

Following gene: 157156116

Centisome position: 45.95

GC content: 34.79

Gene sequence:

>1032_bases
ATGACTAGATATGAAAAAACTATTGAGCAATTAAAAGTTAAACCTAAGACTTGGTTGATAACAGGAGTTGCTGGCTTCAT
TGGCTCAAATTTACTTGAAACACTTTTAAGATTAAATCAGACAGTAGTTGGTTTGGATAATTTTGCTACAGGACATCAGC
GAAATCTTGATGAAGTCCAGCAAAACGTTACACCTGAAGCATGGCAAAAATTTACAATGATCGAAGGTGATATACGTGAT
TATGAGACATGTATGAAGGCCGTTGATAATGTTAACTACGTATTGCACCAGGCTGCACTTGGTTCAGTGCCTCGTTCAAT
TAATGATCCTATAACAACTAATGAAGTTAATGTCTCTGGCTTCTTGAATATGTTACAAGCTGCCAAGTGTAGAGATGTAG
AGAGTTTTGTTTTTGCCGCTTCAAGTTCAACATATGGTGATCATACCGCTCTGCCCAAAATAGAACATACAATTGGTAAA
CCATTATCTCCCTATGCTATCACAAAATATGTAAATGAATTATATGCGGAAGTTTTCGCAAAAATGTATGGTTTTAAAAG
CATTGGTTTGAGATATTTCAATGTCTTTGGTAAAAGGCAAGATCCAAATGGTGCATATGCAGCAGTAATACCCAAATGGA
TATCGGCAATGCTCATGGATAAAGATATTTATATCAATGGTGATGGGGAAACTAGTAGAGATTTTTCATTTATTGAAAAT
ACTGTTCAAATGAATATTCTTGCTGCTACAGCAGATGATAGTGCAAAAGGAGAAGTTTACAACGTTGCTTATGGTGAGAG
AACATCATTAAACGAATTAGTTGAATTTATAAAAAATGACCTTGGCAGTAATGGAATAAAATATGCAGGGAATATTATAT
ATCGGGATTTCAGAAAAGGTGATGTGAGACATTCTCTAGCTGATATTAGTAAAGCTAGAGAAATGCTCGGCTATCAACCT
CAGTTTGATATTAAAACTGGTTTAAGACAGGCAATGCCCTGGTATATAAACTTATTTAAAAAAGCAGAATGA

Upstream 100 bases:

>100_bases
AAATCAAGGAGCTGCAGTCCACAAAATGTGTGACATATGATTTGAAATATATTCTTAATAAATCAGATGCAACAATTAGA
CTTTAACTAGGTGAATATCT

Downstream 100 bases:

>100_bases
CATACGTTTCTAACTAGTTATTTTATATTTTGGCAGATAATCTGCCAAAATATAATGCATTTATTATCTATTACCATCAT
TATGTTACGTATATAAATGA

Product: VI polysaccharide biosynthesis protein vipB/tviC

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVGLDNFATGHQRNLDEVQQNVTPEAWQKFTMIEGDIRD
YETCMKAVDNVNYVLHQAALGSVPRSINDPITTNEVNVSGFLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGK
PLSPYAITKYVNELYAEVFAKMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAMLMDKDIYINGDGETSRDFSFIEN
TVQMNILAATADDSAKGEVYNVAYGERTSLNELVEFIKNDLGSNGIKYAGNIIYRDFRKGDVRHSLADISKAREMLGYQP
QFDIKTGLRQAMPWYINLFKKAE

Sequences:

>Translated_343_residues
MTRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVGLDNFATGHQRNLDEVQQNVTPEAWQKFTMIEGDIRD
YETCMKAVDNVNYVLHQAALGSVPRSINDPITTNEVNVSGFLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGK
PLSPYAITKYVNELYAEVFAKMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAMLMDKDIYINGDGETSRDFSFIEN
TVQMNILAATADDSAKGEVYNVAYGERTSLNELVEFIKNDLGSNGIKYAGNIIYRDFRKGDVRHSLADISKAREMLGYQP
QFDIKTGLRQAMPWYINLFKKAE
>Mature_342_residues
TRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVGLDNFATGHQRNLDEVQQNVTPEAWQKFTMIEGDIRDY
ETCMKAVDNVNYVLHQAALGSVPRSINDPITTNEVNVSGFLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGKP
LSPYAITKYVNELYAEVFAKMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAMLMDKDIYINGDGETSRDFSFIENT
VQMNILAATADDSAKGEVYNVAYGERTSLNELVEFIKNDLGSNGIKYAGNIIYRDFRKGDVRHSLADISKAREMLGYQPQ
FDIKTGLRQAMPWYINLFKKAE

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=30.4878048780488, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=26.0736196319018, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI56237023, Length=321, Percent_Identity=26.1682242990654, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI56118217, Length=321, Percent_Identity=26.1682242990654, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI189083684, Length=321, Percent_Identity=26.1682242990654, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786974, Length=320, Percent_Identity=27.1875, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=27.6967930029155, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=25.5747126436782, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=25.5747126436782, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788589, Length=346, Percent_Identity=23.9884393063584, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=27.6595744680851, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=342, Percent_Identity=26.9005847953216, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=25.7396449704142, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=342, Percent_Identity=26.9005847953216, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=23.9393939393939, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=340, Percent_Identity=25.8823529411765, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=24.3626062322946, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=27.0833333333333, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38617; Mature: 38486

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVGLDNFATGHQRNLDEVQ
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHH
QNVTPEAWQKFTMIEGDIRDYETCMKAVDNVNYVLHQAALGSVPRSINDPITTNEVNVSG
HCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGKPLSPYAITKYVNELYAEVFA
HHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAMLMDKDIYINGDGETSRDFSFIEN
HHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHH
TVQMNILAATADDSAKGEVYNVAYGERTSLNELVEFIKNDLGSNGIKYAGNIIYRDFRKG
HHEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHHHHHHCC
DVRHSLADISKAREMLGYQPQFDIKTGLRQAMPWYINLFKKAE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure 
TRYEKTIEQLKVKPKTWLITGVAGFIGSNLLETLLRLNQTVVGLDNFATGHQRNLDEVQ
CHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHH
QNVTPEAWQKFTMIEGDIRDYETCMKAVDNVNYVLHQAALGSVPRSINDPITTNEVNVSG
HCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FLNMLQAAKCRDVESFVFAASSSTYGDHTALPKIEHTIGKPLSPYAITKYVNELYAEVFA
HHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KMYGFKSIGLRYFNVFGKRQDPNGAYAAVIPKWISAMLMDKDIYINGDGETSRDFSFIEN
HHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHH
TVQMNILAATADDSAKGEVYNVAYGERTSLNELVEFIKNDLGSNGIKYAGNIIYRDFRKG
HHEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHHHHHHCC
DVRHSLADISKAREMLGYQPQFDIKTGLRQAMPWYINLFKKAE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]