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The map label for this gene is rmlA2 [C]

Identifier: 156937947

GI number: 156937947

Start: 1035246

End: 1035944

Strand: Direct

Name: rmlA2 [C]

Synonym: Igni_1159

Alternate gene names: 156937947

Gene position: 1035246-1035944 (Clockwise)

Preceding gene: 156937944

Following gene: 156937948

Centisome position: 79.79

GC content: 53.51

Gene sequence:

>699_bases
GTGAAGGCGTTGATACTCGCCGGGGGGTACGGCAAGAGGCTGAAGCCCCTCACCGAGGAGTTGCCGAAGCCCTTGCTCCA
AGTGGCGGGGAAGCCCATAGTGGCGTGGCAATTCGACCTATACAAGAGACACGGAATAAATGAAATAGTTATGGCAGTGG
GCTACTTGAAGGAAAAAGTAATAGAATTGGTCGGCTCGGGCAGCAAGTACGGCGTGAAGGTAGTTTACGTCGTCGAGGAG
GAGCCCTTGGGGACCGGAGGGGCCATAAAGAACGCTGAGAGCGCGTTGAGAAACGAAGAGATGTTCGCAGTTTCCAACGG
AGATATAATAACGAACTTGGACGTCACCAAGCTCTGCGACGCGTTGAGGGAGAGCGAGGCGGTAGCGGCCATGTCGCTGG
TCCCCCTACCCTCCCCCTACGGAATAGTGATAACTGATGAGAGTGGGAAAGTTAAGGAATTCAAGGAGAAACCGAAGATA
CACGACTACTGGATAAACGCCGGGGTATATTGTATGAGGAACGAGATATTCGATTACTTACCCGAGAGCGGGGACATAGA
GAAGACCGCTTTCCCCCAGCTCGCCAAGGAGGGGAAGCTGGTGGCCGTTAAGTACCCGGACGCCTTCTGGAAGAGCGTGG
ACACGTTTAAGGACTTAGAGGAGGCCAACAAACTGTTGTCGAAGTTGAGCTTGTTCTGA

Upstream 100 bases:

>100_bases
CCCAATATGACTAGCCCTCTCCTCAACCCCTTAATACCTCCTTTACCACAACGCGAGAACCAAAATTAATGCTCGCATAG
GCTCCAGTACGGTGGCCTCC

Downstream 100 bases:

>100_bases
GGTGAGAAGCTTGGACGAGAGGCTGGTGGCTTCCCTCTCCGGGGCCCTCTGCGGGGCCTTCGCCGCGCTGGGGGCACTGT
CCTTGAGCTCCGGCTGGGAG

Product: nucleotidyl transferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKALILAGGYGKRLKPLTEELPKPLLQVAGKPIVAWQFDLYKRHGINEIVMAVGYLKEKVIELVGSGSKYGVKVVYVVEE
EPLGTGGAIKNAESALRNEEMFAVSNGDIITNLDVTKLCDALRESEAVAAMSLVPLPSPYGIVITDESGKVKEFKEKPKI
HDYWINAGVYCMRNEIFDYLPESGDIEKTAFPQLAKEGKLVAVKYPDAFWKSVDTFKDLEEANKLLSKLSLF

Sequences:

>Translated_232_residues
MKALILAGGYGKRLKPLTEELPKPLLQVAGKPIVAWQFDLYKRHGINEIVMAVGYLKEKVIELVGSGSKYGVKVVYVVEE
EPLGTGGAIKNAESALRNEEMFAVSNGDIITNLDVTKLCDALRESEAVAAMSLVPLPSPYGIVITDESGKVKEFKEKPKI
HDYWINAGVYCMRNEIFDYLPESGDIEKTAFPQLAKEGKLVAVKYPDAFWKSVDTFKDLEEANKLLSKLSLF
>Mature_232_residues
MKALILAGGYGKRLKPLTEELPKPLLQVAGKPIVAWQFDLYKRHGINEIVMAVGYLKEKVIELVGSGSKYGVKVVYVVEE
EPLGTGGAIKNAESALRNEEMFAVSNGDIITNLDVTKLCDALRESEAVAAMSLVPLPSPYGIVITDESGKVKEFKEKPKI
HDYWINAGVYCMRNEIFDYLPESGDIEKTAFPQLAKEGKLVAVKYPDAFWKSVDTFKDLEEANKLLSKLSLF

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=239, Percent_Identity=33.4728033472803, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI11761619, Length=239, Percent_Identity=33.4728033472803, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI31881779, Length=266, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI45447090, Length=266, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI262205273, Length=221, Percent_Identity=24.4343891402715, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI9966779, Length=221, Percent_Identity=24.4343891402715, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790224, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788351, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=238, Percent_Identity=37.8151260504202, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17509979, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509981, Length=233, Percent_Identity=28.3261802575107, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=229, Percent_Identity=36.6812227074236, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21355443, Length=226, Percent_Identity=36.283185840708, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24644084, Length=226, Percent_Identity=36.283185840708, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24653912, Length=199, Percent_Identity=29.6482412060301, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 25702; Mature: 25702

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALILAGGYGKRLKPLTEELPKPLLQVAGKPIVAWQFDLYKRHGINEIVMAVGYLKEKV
CCEEEEECCCCCCCCHHHHHCCHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHH
IELVGSGSKYGVKVVYVVEEEPLGTGGAIKNAESALRNEEMFAVSNGDIITNLDVTKLCD
HHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCEEECCCHHHHHH
ALRESEAVAAMSLVPLPSPYGIVITDESGKVKEFKEKPKIHDYWINAGVYCMRNEIFDYL
HHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHC
PESGDIEKTAFPQLAKEGKLVAVKYPDAFWKSVDTFKDLEEANKLLSKLSLF
CCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKALILAGGYGKRLKPLTEELPKPLLQVAGKPIVAWQFDLYKRHGINEIVMAVGYLKEKV
CCEEEEECCCCCCCCHHHHHCCHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHH
IELVGSGSKYGVKVVYVVEEEPLGTGGAIKNAESALRNEEMFAVSNGDIITNLDVTKLCD
HHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCEEECCCHHHHHH
ALRESEAVAAMSLVPLPSPYGIVITDESGKVKEFKEKPKIHDYWINAGVYCMRNEIFDYL
HHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHC
PESGDIEKTAFPQLAKEGKLVAVKYPDAFWKSVDTFKDLEEANKLLSKLSLF
CCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA