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The map label for this gene is rtcA

Identifier: 156937325

GI number: 156937325

Start: 474957

End: 476018

Strand: Reverse

Name: rtcA

Synonym: Igni_0532

Alternate gene names: 156937325

Gene position: 476018-474957 (Counterclockwise)

Preceding gene: 156937326

Following gene: 156937323

Centisome position: 36.69

GC content: 61.21

Gene sequence:

>1062_bases
ATGATCGAGATAGACGGCTCCTTCGGCGAGGGAGGTGGCCAAATACTTAGGACCTCCGTGGCACTCTCTGCCGTGACTTT
GAAACCGGTTAGAATATTCAACATCAGGGCTAAGAGGAAGAACCCGGGCTTGAGGAGGCAACACATGGTGGCCGTCAAGG
CCTTGGCCGAGATGACCGACGCGGAGGTCCGCGGCTTGGAGCTGGGCTCCACCGAAATAGTGTTCATACCGAAGACTTTG
AAGGCCGGGACGTTTAGGTTCGACATAGGCACCGCGGGCAGCGTCAGCTTGGTCTTGCAAGCAGTAACTCCCGCGGCTCT
CTTCGCCCCCGGAGAGGTCAGGGTTCAGCTGAGAGGGGGTACGGACGTGCCGATGAGCCCTCCGGTGGACTACTTGAGGT
TCGTCTTCTATCCCCTCCTGGAGAGGTTCGGCGCCAAGACGGAGTTGGTCCTCAAGCGGCGAGGCCACTACCCCAAGGGA
GGGGGAGAGGTCGAGTTCGCCTCCCGCCCTGTGGACTCACTGACCCAGTGGGGGGAGGTGGAGCGGGGCGAGGTGCTGAA
GGTCCGCGGGCTCTCTCACTGCGTCAAGCTCCCTAAGCACGTCGCGGAGAGGCAAGCGAAGGCCGCCGAAGAGGTCCTCA
AGAAGAGCGGCCTAAAGAACGTAGACATCGACTTGGAGTGGTACCCTCCGGAGAGGGACCCCCACTTGGGGCCCGGGAGC
GGGATAGTGCTTTGGGCCATAACGGAGAGGAGCTTGTTGGGCGCGGACTCGTTGGGAGCTAGGGGGAAGAGGGCCGAGAG
GGTGGGCGAGGAGGCGGCGCGGAAGCTCTTGGAGGACTTGAGCACGGGCAAGGCTTTGGACAGGCACATGAGCGACATGA
TAGTTCCTTACGTCTCCTTAGCTTGCGGAAGGACCGAGGTGGGAGGCGCGGCCCTTACCATGCACGCTTGGACCCACGTG
CACGTGGTTAAGAAGTTCCTCCCGGAGCTCGAGGTGGAAATATCGGGTGAGCTGAACAAGCCATTCGTTATGAGGGTAAA
GGGCGTGTGTTGGCAGCGCTAG

Upstream 100 bases:

>100_bases
AGAGGCCGGGCGTGGAATACTTGCCCGTCCCGGCGGTCCCCCAAAAGAGGGGGCTCGGCGATAAAGCCTAACCCTCACAC
GACTTTTTAGGAGCGACGAC

Downstream 100 bases:

>100_bases
GGCACCCTATCCGCGTAGAGCTCCCAGACCCTCTGGCAGTGGGGGCCCCACTCGACCCTCACCGGGTTCAAGAGGCCGGC
CCTGATCGCGTGATCCACCA

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MIEIDGSFGEGGGQILRTSVALSAVTLKPVRIFNIRAKRKNPGLRRQHMVAVKALAEMTDAEVRGLELGSTEIVFIPKTL
KAGTFRFDIGTAGSVSLVLQAVTPAALFAPGEVRVQLRGGTDVPMSPPVDYLRFVFYPLLERFGAKTELVLKRRGHYPKG
GGEVEFASRPVDSLTQWGEVERGEVLKVRGLSHCVKLPKHVAERQAKAAEEVLKKSGLKNVDIDLEWYPPERDPHLGPGS
GIVLWAITERSLLGADSLGARGKRAERVGEEAARKLLEDLSTGKALDRHMSDMIVPYVSLACGRTEVGGAALTMHAWTHV
HVVKKFLPELEVEISGELNKPFVMRVKGVCWQR

Sequences:

>Translated_353_residues
MIEIDGSFGEGGGQILRTSVALSAVTLKPVRIFNIRAKRKNPGLRRQHMVAVKALAEMTDAEVRGLELGSTEIVFIPKTL
KAGTFRFDIGTAGSVSLVLQAVTPAALFAPGEVRVQLRGGTDVPMSPPVDYLRFVFYPLLERFGAKTELVLKRRGHYPKG
GGEVEFASRPVDSLTQWGEVERGEVLKVRGLSHCVKLPKHVAERQAKAAEEVLKKSGLKNVDIDLEWYPPERDPHLGPGS
GIVLWAITERSLLGADSLGARGKRAERVGEEAARKLLEDLSTGKALDRHMSDMIVPYVSLACGRTEVGGAALTMHAWTHV
HVVKKFLPELEVEISGELNKPFVMRVKGVCWQR
>Mature_353_residues
MIEIDGSFGEGGGQILRTSVALSAVTLKPVRIFNIRAKRKNPGLRRQHMVAVKALAEMTDAEVRGLELGSTEIVFIPKTL
KAGTFRFDIGTAGSVSLVLQAVTPAALFAPGEVRVQLRGGTDVPMSPPVDYLRFVFYPLLERFGAKTELVLKRRGHYPKG
GGEVEFASRPVDSLTQWGEVERGEVLKVRGLSHCVKLPKHVAERQAKAAEEVLKKSGLKNVDIDLEWYPPERDPHLGPGS
GIVLWAITERSLLGADSLGARGKRAERVGEEAARKLLEDLSTGKALDRHMSDMIVPYVSLACGRTEVGGAALTMHAWTHV
HVVKKFLPELEVEISGELNKPFVMRVKGVCWQR

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=327, Percent_Identity=38.5321100917431, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI195927044, Length=340, Percent_Identity=37.0588235294118, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI157426877, Length=349, Percent_Identity=26.3610315186246, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI48994937, Length=334, Percent_Identity=39.5209580838323, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32564710, Length=303, Percent_Identity=25.4125412541254, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324564, Length=304, Percent_Identity=24.0131578947368, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18543269, Length=330, Percent_Identity=33.9393939393939, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI18860065, Length=301, Percent_Identity=27.2425249169435, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_IGNH4 (A8A9W2)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435121.1
- ProteinModelPortal:   A8A9W2
- SMR:   A8A9W2
- STRING:   A8A9W2
- GeneID:   5562318
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0532
- eggNOG:   arNOG04553
- HOGENOM:   HBG683539
- OMA:   VGSAGSC
- ProtClustDB:   PRK04204
- BioCyc:   IHOS453591:IGNI_0532-MONOMER
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 38696; Mature: 38696

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 315-315 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIDGSFGEGGGQILRTSVALSAVTLKPVRIFNIRAKRKNPGLRRQHMVAVKALAEMTD
CEEECCCCCCCCCHHHHHHHHHEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AEVRGLELGSTEIVFIPKTLKAGTFRFDIGTAGSVSLVLQAVTPAALFAPGEVRVQLRGG
HHHCCEECCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCHHEECCCEEEEEEECC
TDVPMSPPVDYLRFVFYPLLERFGAKTELVLKRRGHYPKGGGEVEFASRPVDSLTQWGEV
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCEEEECCCCHHHHHHCCCC
ERGEVLKVRGLSHCVKLPKHVAERQAKAAEEVLKKSGLKNVDIDLEWYPPERDPHLGPGS
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
GIVLWAITERSLLGADSLGARGKRAERVGEEAARKLLEDLSTGKALDRHMSDMIVPYVSL
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ACGRTEVGGAALTMHAWTHVHVVKKFLPELEVEISGELNKPFVMRVKGVCWQR
HCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHCCC
>Mature Secondary Structure
MIEIDGSFGEGGGQILRTSVALSAVTLKPVRIFNIRAKRKNPGLRRQHMVAVKALAEMTD
CEEECCCCCCCCCHHHHHHHHHEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AEVRGLELGSTEIVFIPKTLKAGTFRFDIGTAGSVSLVLQAVTPAALFAPGEVRVQLRGG
HHHCCEECCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCHHEECCCEEEEEEECC
TDVPMSPPVDYLRFVFYPLLERFGAKTELVLKRRGHYPKGGGEVEFASRPVDSLTQWGEV
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCEEEECCCCHHHHHHCCCC
ERGEVLKVRGLSHCVKLPKHVAERQAKAAEEVLKKSGLKNVDIDLEWYPPERDPHLGPGS
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
GIVLWAITERSLLGADSLGARGKRAERVGEEAARKLLEDLSTGKALDRHMSDMIVPYVSL
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ACGRTEVGGAALTMHAWTHVHVVKKFLPELEVEISGELNKPFVMRVKGVCWQR
HCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA