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The map label for this gene is purA [H]

Identifier: 156937140

GI number: 156937140

Start: 304343

End: 305362

Strand: Reverse

Name: purA [H]

Synonym: Igni_0346

Alternate gene names: 156937140

Gene position: 305362-304343 (Counterclockwise)

Preceding gene: 156937141

Following gene: 156937139

Centisome position: 23.53

GC content: 59.31

Gene sequence:

>1020_bases
GTGCCAGTTAGGCTCGTGGTAGGCGCGCTCTTCGGGGACGAGGGTAAGGGCAAAGTAGCGGCTTACCTATCCTTCGTCGA
GAGGCCCGAGTTCCTAGTGAGGACCGGGGCGATAAACGCTGGCCACACGGTAGTACACATGGGTAAGGAGTACAAGCTTA
GGGCCTTGCCCTCAGGGACGGTGACGTGTGACGTCTGCAAGGCCGCCGTCGCCCCGGGAGCTTTGATAAGCGTAGGGGTT
CTGGCGAACGAAGTGAAGGAGCTCGGAATTTCGAAAGAGAGGGTATTCGTTGACGAAAATACCGGAATTATTGAGGAAGA
GCACGTCAGGAGGGAGAGGGAGGACGCCCACTTGAGGTCGAGGATAGGCTCGACCCTCACGGGAGTCGGGGCAGCGATGA
GCGACAGAGTAATGAGGAGGCTCAAGCTAGCCAGAGACTTCAAAGAAGAAATTGAAAAGTACGCAACGGTTACGGACGTG
CCCTCATTGCTCTACGACGCGGTGGATTCTTATAAAGTGGTCCACGTGGAGGGCACACAAGGCTTCGGGTTGAGCTTGTT
CCACGGCACGTACCCGTACGTGACCTCTAGGGACGTCACCGCCGGGGCCCTCCTAAGCGAGACCGGGCTGGCCCCTTCGG
ACGTGGAGGACGTCATATTGGTCACTAAGTCTTTCGTAACGAGGGTCGGGGAGGGACCCCTCAAGGGAGAGCTGAGCTTG
GAGGAGCTCCAGAGGAGGGGCATGGTAGAGAGGGGCACTGTAACCGGCAGGCCGAGGAGGGCGGCCCCTCTGGAGGAGAA
CTTGGACTTACTCAAGAGGGCGGCGAGGGCCAACGGCGCGACCAAAATTGCAATAACGAAGATAGACGTACTGTTCCCCA
AGGCGAAGGGGGTCACCTCGTGGAGCGACTTGCCGCCCGAGGCGAGGCTCTGGATCAGCGATATAGAGAAAGAGGTGGGC
GTCCCGGTCTGTTACGTGGGAACCGGCCCGGAAGCTCTGGAGACCGTGGGGGTGTGTTGA

Upstream 100 bases:

>100_bases
GGGGCCGGGCAAGAAATACCTCGAGGAAATGAAGGAGATACAGATAACTAGGTGAAGTTTTATATCCTGGACGCGCGAGC
GCGCCCACAAGGGTACTTGG

Downstream 100 bases:

>100_bases
GGTGAAGGAGTACGACGTCCTAATAGTGGGGGCTGGCCCCGCGGGGATGTTCGCGGCCCACGAGCTGGCCACCTTAGGGG
AGGGCAAGCTGAGGGTAGGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPVRLVVGALFGDEGKGKVAAYLSFVERPEFLVRTGAINAGHTVVHMGKEYKLRALPSGTVTCDVCKAAVAPGALISVGV
LANEVKELGISKERVFVDENTGIIEEEHVRREREDAHLRSRIGSTLTGVGAAMSDRVMRRLKLARDFKEEIEKYATVTDV
PSLLYDAVDSYKVVHVEGTQGFGLSLFHGTYPYVTSRDVTAGALLSETGLAPSDVEDVILVTKSFVTRVGEGPLKGELSL
EELQRRGMVERGTVTGRPRRAAPLEENLDLLKRAARANGATKIAITKIDVLFPKAKGVTSWSDLPPEARLWISDIEKEVG
VPVCYVGTGPEALETVGVC

Sequences:

>Translated_339_residues
MPVRLVVGALFGDEGKGKVAAYLSFVERPEFLVRTGAINAGHTVVHMGKEYKLRALPSGTVTCDVCKAAVAPGALISVGV
LANEVKELGISKERVFVDENTGIIEEEHVRREREDAHLRSRIGSTLTGVGAAMSDRVMRRLKLARDFKEEIEKYATVTDV
PSLLYDAVDSYKVVHVEGTQGFGLSLFHGTYPYVTSRDVTAGALLSETGLAPSDVEDVILVTKSFVTRVGEGPLKGELSL
EELQRRGMVERGTVTGRPRRAAPLEENLDLLKRAARANGATKIAITKIDVLFPKAKGVTSWSDLPPEARLWISDIEKEVG
VPVCYVGTGPEALETVGVC
>Mature_338_residues
PVRLVVGALFGDEGKGKVAAYLSFVERPEFLVRTGAINAGHTVVHMGKEYKLRALPSGTVTCDVCKAAVAPGALISVGVL
ANEVKELGISKERVFVDENTGIIEEEHVRREREDAHLRSRIGSTLTGVGAAMSDRVMRRLKLARDFKEEIEKYATVTDVP
SLLYDAVDSYKVVHVEGTQGFGLSLFHGTYPYVTSRDVTAGALLSETGLAPSDVEDVILVTKSFVTRVGEGPLKGELSLE
ELQRRGMVERGTVTGRPRRAAPLEENLDLLKRAARANGATKIAITKIDVLFPKAKGVTSWSDLPPEARLWISDIEKEVGV
PVCYVGTGPEALETVGVC

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=29.2161520190024, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI34577063, Length=423, Percent_Identity=28.1323877068558, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI40316944, Length=387, Percent_Identity=28.1653746770026, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=31.7647058823529, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146555, Length=422, Percent_Identity=29.8578199052133, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146553, Length=422, Percent_Identity=29.8578199052133, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=29.7423887587822, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=29.9287410926366, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 36657; Mature: 36525

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRLVVGALFGDEGKGKVAAYLSFVERPEFLVRTGAINAGHTVVHMGKEYKLRALPSGT
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCEEEEECCCCC
VTCDVCKAAVAPGALISVGVLANEVKELGISKERVFVDENTGIIEEEHVRREREDAHLRS
EEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RIGSTLTGVGAAMSDRVMRRLKLARDFKEEIEKYATVTDVPSLLYDAVDSYKVVHVEGTQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
GFGLSLFHGTYPYVTSRDVTAGALLSETGLAPSDVEDVILVTKSFVTRVGEGPLKGELSL
CCCEEEECCCCCCEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
EELQRRGMVERGTVTGRPRRAAPLEENLDLLKRAARANGATKIAITKIDVLFPKAKGVTS
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCC
WSDLPPEARLWISDIEKEVGVPVCYVGTGPEALETVGVC
CCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
PVRLVVGALFGDEGKGKVAAYLSFVERPEFLVRTGAINAGHTVVHMGKEYKLRALPSGT
CCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCEEEEECCCCC
VTCDVCKAAVAPGALISVGVLANEVKELGISKERVFVDENTGIIEEEHVRREREDAHLRS
EEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RIGSTLTGVGAAMSDRVMRRLKLARDFKEEIEKYATVTDVPSLLYDAVDSYKVVHVEGTQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
GFGLSLFHGTYPYVTSRDVTAGALLSETGLAPSDVEDVILVTKSFVTRVGEGPLKGELSL
CCCEEEECCCCCCEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
EELQRRGMVERGTVTGRPRRAAPLEENLDLLKRAARANGATKIAITKIDVLFPKAKGVTS
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCC
WSDLPPEARLWISDIEKEVGVPVCYVGTGPEALETVGVC
CCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA