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The map label for this gene is nfi

Identifier: 156937131

GI number: 156937131

Start: 295689

End: 296324

Strand: Reverse

Name: nfi

Synonym: Igni_0337

Alternate gene names: 156937131

Gene position: 296324-295689 (Counterclockwise)

Preceding gene: 156937132

Following gene: 156937127

Centisome position: 22.84

GC content: 62.74

Gene sequence:

>636_bases
ATGACTTTCGACCGGAGGAGGGCCTCCGAGCTCCAGAGGAAGCTCTCCGAGCTGGTGAAGGAGGAGGACTGCTTCGATCC
GGAGGCGGTAGAGGCGGTGGGCGGGCTGGACGTCTCCTACAAGGGCGACGTCGGCGTCTCCGCGCTCTCGCTGATCGACT
ATAAAACCTTAAGGCCTCTCAAGCACTACTACGTCGTCGCGAGGGTCCCCATCCCCTACGTGCCGGGGTTCTTGGCCTTC
CGGGAGGCACCCTTGCACTTGACGTTAATTAAGAAGGTCAAGGGCTACGACTTGCTCTTGGTGGACGGCCACGGGAGGAC
CCACCCCAGGGGGCTGGGGATAGCCTCCCACGTGGGGGTGACGAGCGGGGTCCCCACGGTCGGGGTGGCAAAGAGGAGGC
TGGTGGGGGAGGAGGAGAGGTGCGGGGAGAGGGAGTGCTTGGTGCACGAGGGTAAGGTGGTGGCTTACGTCATCAGGAGA
GGAAAGCAAAAGCTGTACGTCAGCGTCGGACACTGCGTGAGCTTGGAGACCGCCTACCAAATAGTGAAGAGGTTAACTGT
CCGGAGGCTGCCGGAGCCGATAGCGTGGGCGGACCGAATCTCCCGTTCGCTGGCCCGCTCACTCCAGCTCCCTTAA

Upstream 100 bases:

>100_bases
TGAAGGAGATAGTTGAGGAGTGCGAGAGGATAGGCTCCGAGTTCGGCCCCTTGCTGGCGAGCTTCCCCCGGAGGGGGGTC
GAGCTAATGGGAGTGAAACT

Downstream 100 bases:

>100_bases
GGCCTTAACGCACATCTCGTAGTGCTCTTCGTCCATCAAGCCCTCCTCAAAGGCGAGCTTGCAGAAGCAGCTCCCGTGAG
GCCTCATCTCCCCCTCCGAT

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTFDRRRASELQRKLSELVKEEDCFDPEAVEAVGGLDVSYKGDVGVSALSLIDYKTLRPLKHYYVVARVPIPYVPGFLAF
REAPLHLTLIKKVKGYDLLLVDGHGRTHPRGLGIASHVGVTSGVPTVGVAKRRLVGEEERCGERECLVHEGKVVAYVIRR
GKQKLYVSVGHCVSLETAYQIVKRLTVRRLPEPIAWADRISRSLARSLQLP

Sequences:

>Translated_211_residues
MTFDRRRASELQRKLSELVKEEDCFDPEAVEAVGGLDVSYKGDVGVSALSLIDYKTLRPLKHYYVVARVPIPYVPGFLAF
REAPLHLTLIKKVKGYDLLLVDGHGRTHPRGLGIASHVGVTSGVPTVGVAKRRLVGEEERCGERECLVHEGKVVAYVIRR
GKQKLYVSVGHCVSLETAYQIVKRLTVRRLPEPIAWADRISRSLARSLQLP
>Mature_210_residues
TFDRRRASELQRKLSELVKEEDCFDPEAVEAVGGLDVSYKGDVGVSALSLIDYKTLRPLKHYYVVARVPIPYVPGFLAFR
EAPLHLTLIKKVKGYDLLLVDGHGRTHPRGLGIASHVGVTSGVPTVGVAKRRLVGEEERCGERECLVHEGKVVAYVIRRG
KQKLYVSVGHCVSLETAYQIVKRLTVRRLPEPIAWADRISRSLARSLQLP

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=216, Percent_Identity=35.6481481481481, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI257467548, Length=134, Percent_Identity=38.0597014925373, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI257467550, Length=134, Percent_Identity=38.0597014925373, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI87082357, Length=202, Percent_Identity=39.1089108910891, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71981509, Length=219, Percent_Identity=29.6803652968037, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_IGNH4 (A8A9B8)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434927.1
- ProteinModelPortal:   A8A9B8
- STRING:   A8A9B8
- GeneID:   5562626
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0337
- eggNOG:   arNOG11595
- HOGENOM:   HBG716360
- OMA:   SVGHRIG
- BioCyc:   IHOS453591:IGNI_0337-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 23552; Mature: 23421

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDRRRASELQRKLSELVKEEDCFDPEAVEAVGGLDVSYKGDVGVSALSLIDYKTLRPL
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
KHYYVVARVPIPYVPGFLAFREAPLHLTLIKKVKGYDLLLVDGHGRTHPRGLGIASHVGV
HHEEEEEECCCCCCCCHHHHHCCCEEEEEEHHCCCCEEEEECCCCCCCCCCCCHHHHCCC
TSGVPTVGVAKRRLVGEEERCGERECLVHEGKVVAYVIRRGKQKLYVSVGHCVSLETAYQ
CCCCCCHHHHHHHHCCCHHHCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCEECHHHHHH
IVKRLTVRRLPEPIAWADRISRSLARSLQLP
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFDRRRASELQRKLSELVKEEDCFDPEAVEAVGGLDVSYKGDVGVSALSLIDYKTLRPL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
KHYYVVARVPIPYVPGFLAFREAPLHLTLIKKVKGYDLLLVDGHGRTHPRGLGIASHVGV
HHEEEEEECCCCCCCCHHHHHCCCEEEEEEHHCCCCEEEEECCCCCCCCCCCCHHHHCCC
TSGVPTVGVAKRRLVGEEERCGERECLVHEGKVVAYVIRRGKQKLYVSVGHCVSLETAYQ
CCCCCCHHHHHHHHCCCHHHCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCEECHHHHHH
IVKRLTVRRLPEPIAWADRISRSLARSLQLP
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA