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The map label for this gene is hasA

Identifier: 15675933

GI number: 15675933

Start: 1832625

End: 1833884

Strand: Direct

Name: hasA

Synonym: SPy_2200

Alternate gene names: 15675933

Gene position: 1832625-1833884 (Clockwise)

Preceding gene: 15675924

Following gene: 15675934

Centisome position: 98.93

GC content: 32.54

Gene sequence:

>1260_bases
GTGCCTATTTTTAAAAAAACTTTAATTGTTTTATCCTTTATTTTTTTGATATCTATCTTGATTTATCTAAATATGTATCT
ATTTGGAACATCAACTGTAGGAATTTATGGAGTAATATTAATAACCTATCTAGTTATTAAACTTGGATTATCTTTCCTTT
ATGAGCCATTTAAAGGAAAGCCACATGACTATAAAGTTGCTGCTGTAATTCCTTCTTATAATGAAGATGCCGAGTCATTA
TTAGAAACTCTTAAAAGTGTGTTAGCACAGACCTATCCGTTATCAGAAATTTATATTGTTGATGATGGGAGTTCAAACAC
AGATGCAATACAATTAATTGAAGAGTATGTAAATAGAGAAGTGGATATTTGTCGAAACGTTATCGTTCACCGTTCCCTTG
TCAATAAAGGAAAACGCCATGCTCAAGCGTGGGCATTTGAAAGATCTGACGCTGACGTTTTTTTAACCGTAGATTCAGAT
ACTTATATCTATCCAAATGCCTTAGAAGAACTCCTAAAAAGCTTCAATGATGAGACAGTTTATGCTGCAACAGGACATTT
GAATGCTAGAAACAGACAAACTAATCTATTAACGCGACTTACAGATATCCGTTACGATAATGCCTTTGGGGTGGAGCGTG
CTGCTCAATCATTAACAGGTAATATTTTAGTTTGCTCAGGACCATTGAGTATTTATCGACGTGAAGTGATTATTCCTAAC
TTAGAGCGCTATAAAAATCAAACATTCCTAGGTTTACCTGTTAGCATTGGGGATGATCGATGTTTAACAAATTATGCTAT
TGATTTAGGACGCACTGTCTACCAATCAACAGCTAGATGTGATACTGATGTACCTTTCCAATTAAAAAGTTATTTAAAGC
AACAAAATCGATGGAATAAATCTTTTTTTAAAGAATCTATTATTTCTGTTAAAAAAATTCTTTCTAATCCCATCGTTGCC
TTATGGACTATTTTCGAAGTCGTTATGTTTATGATGTTGATTGTCGCAATTGGGAATCTTTTGTTTAATCAAGCTATTCA
ATTAGACCTTATTAAACTTTTTGCCTTTTTATCCATCATCTTTATCGTTGCTTTATGTCGTAATGTTCATTATATGATCA
AACATCCTGCTAGTTTTTTGTTATCTCCTCTGTATGGAATATTACACTTGTTTGTCTTACAGCCCCTAAAACTTTATTCT
TTATGCACCATTAAAAATACGGAATGGGGAACACGTAAAAAGGTCACTATTTTTAAATAA

Upstream 100 bases:

>100_bases
TTAAAAATATTTCTATGACTAGTTGACATTACCCCTTATTTATATTAGAATATCGAGGTCCCTGTCTTTCAAGGAAATTA
AAAAAGAAAGAGGTGTAATT

Downstream 100 bases:

>100_bases
TATGTGCATCGAGTAGTTAGAGAAGGAGTAATTTTATGAAAATAGCAGTTGCTGGATCAGGATATGTTGGATTATCACTA
GGAGTTCTTTTATCACTTCA

Product: hyaluronate synthase

Products: NA

Alternate protein names: Hyaluronate synthase; Hyaluronic acid synthase; HA synthase

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLVIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESL
LETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSD
TYIYPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPN
LERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFKESIISVKKILSNPIVA
LWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYS
LCTIKNTEWGTRKKVTIFK

Sequences:

>Translated_419_residues
MPIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLVIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESL
LETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSD
TYIYPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPN
LERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFKESIISVKKILSNPIVA
LWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYS
LCTIKNTEWGTRKKVTIFK
>Mature_418_residues
PIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLVIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESLL
ETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSDT
YIYPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPNL
ERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFKESIISVKKILSNPIVAL
WTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYSL
CTIKNTEWGTRKKVTIFK

Specific function: Glycosaminoglycan synthesis. The hyaluronic acid capsule is involved in the pathogenicity of group A Streptococci; it may be the major virulence determinant

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nodC/HAS family

Homologues:

Organism=Homo sapiens, GI20302153, Length=438, Percent_Identity=27.8538812785388, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4885391, Length=434, Percent_Identity=27.8801843317972, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI167466171, Length=452, Percent_Identity=27.212389380531, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787259, Length=261, Percent_Identity=22.9885057471264, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HASA_STRP1 (P0C0H1)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270107.1
- RefSeq:   YP_283214.1
- ProteinModelPortal:   P0C0H1
- EnsemblBacteria:   EBSTRT00000000608
- EnsemblBacteria:   EBSTRT00000027847
- GeneID:   3571023
- GeneID:   901875
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2200
- KEGG:   spz:M5005_Spy_1851
- GeneTree:   EBGT00050000026831
- HOGENOM:   HBG339192
- OMA:   WYHQTFL
- ProtClustDB:   CLSK687618
- BioCyc:   SPYO160490:SPY2200-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1851-MONOMER
- GO:   GO:0009405
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: =2.4.1.212

Molecular weight: Translated: 47887; Mature: 47755

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe6d323c)-; HASH(0xf00cbc4)-; HASH(0xe0e92d4)-; HASH(0xefdc5d4)-; HASH(0xcf365b0)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLVIKLGLSFLYEPFKGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PHDYKVAAVIPSYNEDAESLLETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNRE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCH
VDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSDTYIYPNALEELLKSFNDETV
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCEECHHHHHHHHHHCCCCEE
YAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPN
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCC
LERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNK
HHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCH
SFFKESIISVKKILSNPIVALWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSII
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIVALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEH
>Mature Secondary Structure 
PIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLVIKLGLSFLYEPFKGK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PHDYKVAAVIPSYNEDAESLLETLKSVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNRE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCH
VDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSDTYIYPNALEELLKSFNDETV
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCEECHHHHHHHHHHCCCCEE
YAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPN
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCC
LERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNK
HHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCH
SFFKESIISVKKILSNPIVALWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSII
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIVALCRNVHYMIKHPASFLLSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11296296