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The map label for this gene is glnA [H]

Identifier: 15675696

GI number: 15675696

Start: 1558037

End: 1559383

Strand: Reverse

Name: glnA [H]

Synonym: SPy_1877

Alternate gene names: 15675696

Gene position: 1559383-1558037 (Counterclockwise)

Preceding gene: 15675697

Following gene: 15675693

Centisome position: 84.18

GC content: 37.71

Gene sequence:

>1347_bases
ATGGCAATAACAGTAGCTGACATTCGTCGTGAAGTCAAAGAAAAAAATGTAACGTTTCTTCGCTTGATGTTCACTGATAT
CATGGGCGTTATGAAAAATGTGGAGATTCCTGCAACTAAAGAACAGTTAGACAAAGTATTGTCTAACAAGGTTATGTTTG
ATGGTTCATCTATCGAAGGTTTTGTACGGATCAATGAGTCAGATATGTACCTTTACCCCGATTTAGACACTTGGATTGTT
TTTCCCTGGGGAGATGAAAATGGAGCAGTTGCAGGTTTAATTTGTGATATTTATACAGCAGAAGGAAAGCCTTTTGCAGG
AGATCCTAGAGGAAATTTAAAAAGAGCCCTGAAACACATGAACGAGATCGGCTACAAATCATTTAATCTTGGACCAGAAC
CAGAATTTTTCCTTTTTAAGATGGATGATAAAGGTAATCCGACACTTGAAGTTAACGATAATGGTGGTTATTTTGATTTA
GCGCCAATTGACTTAGCAGACAACACGCGCCGTGAAATTGTGAATATTTTAACGAAAATGGGTTTTGAAGTGGAAGCTAG
TCATCATGAAGTGGCTGTTGGTCAACATGAGATTGATTTTAAATATGCAGATGTTTTGAAAGCTTGTGATAATATTCAAA
TTTTTAAGCTAGTTGTAAAAACGATTGCCCGTGAACATGGACTTTATGCTACTTTCATGGCTAAACCAAAATTTGGAATA
GCTGGATCAGGGATGCACTGTAACATGTCTTTGTTTGATAACCAAGGTAATAATGCTTTTTATGATGAAGCTGATAAGCG
AGGGATGCAGTTATCAGAAGATGCTTATTATTTCTTGGGAGGACTAATGAAGCATGCTTATAACTACACTGCTATCACTA
ACCCTACAGTGAATTCTTATAAACGATTAGTTCCAGGTTATGAGGCACCTGTTTATGTCGCTTGGGCTGGAAGTAATCGT
TCACCGCTTATCCGTGTTCCAGCATCACGTGGTATGGGAACGCGTTTGGAGTTACGTTCGGTTGATCCGACAGCTAATCC
TTATTTAGCCTTGGCTGTTCTCTTGGAAGCTGGATTAGATGGTATCATTAACAAAATTGAAGCTCCAGAACCCGTTGAAG
CTAACATTTATACCATGACAATGGAAGAACGAAATGAAGCAGGCATTATTGATTTGCCATCAACGCTTCATAATGCCTTA
AAAGCTCTTCAAAAAGATGATGTGGTACAAAAGGCACTAGGTTACCATATCTACACTAATTTCTTAGAAGCAAAACGAAT
TGAATGGTCTTCCTATGCAACTTTTGTTTCTCAATGGGAAATTGACCATTATATTCATAATTATTAG

Upstream 100 bases:

>100_bases
GAAATGCTGACACAAAGCGGTTTTTCTACTCCCTCACAGCATATTGGTAATTTTCGTATTTAAGATATGAATTGTATAAA
ATTTAAAGGAGACCTATTTA

Downstream 100 bases:

>100_bases
ACTTATCAGGACCTGCTCAAGTTGAGTGGGTCTTTTGGATGAGCTATTGTATTTGAGCTAGCAAGCTATTATTAGCTGAT
CGGACACCAATTGATTTTCC

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
AGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNR
SPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNAL
KALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY

Sequences:

>Translated_448_residues
MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
AGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNR
SPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNAL
KALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY
>Mature_447_residues
AITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVF
PWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDLA
PIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGIA
GSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAYYFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRS
PLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNALK
ALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=444, Percent_Identity=27.027027027027, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1790301, Length=435, Percent_Identity=42.5287356321839, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI87081870, Length=455, Percent_Identity=25.2747252747253, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50441; Mature: 50310

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00180 GLNA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEG
CEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKHM
EEEECCCCEEEECCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
NEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDLAPIDLADNTRREIVNILTKM
HHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAYYFLGGLMKHAYNYTAITNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHH
KRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLD
HHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCHH
GIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNALKALQKDDVVQKALGYHIYTN
HHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWSSYATFVSQWEIDHYIHNY
HHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLDKVLSNKVMFDGSSIEG
EEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEGKPFAGDPRGNLKRALKHM
EEEECCCCEEEECCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
NEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDLAPIDLADNTRREIVNILTKM
HHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLVVKTIAREHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAYYFLGGLMKHAYNYTAITNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHH
KRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGTRLELRSVDPTANPYLALAVLLEAGLD
HHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCHH
GIINKIEAPEPVEANIYTMTMEERNEAGIIDLPSTLHNALKALQKDDVVQKALGYHIYTN
HHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWSSYATFVSQWEIDHYIHNY
HHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]