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The map label for this gene is nadE

Identifier: 15675523

GI number: 15675523

Start: 1371346

End: 1372170

Strand: Reverse

Name: nadE

Synonym: SPy_1652

Alternate gene names: 15675523

Gene position: 1372170-1371346 (Counterclockwise)

Preceding gene: 15675524

Following gene: 15675522

Centisome position: 74.07

GC content: 42.42

Gene sequence:

>825_bases
ATGACACTACAAGAAGAGATAATTCGACAATTAGGCGTCAAGGCTAGCATTGACCCACAAGAAGAAATTCGGAAAGCCGT
TGATTTTTTAAAAGCTTATTTGCGTAAGCACTCATTTTTAAAAACTTATGTCTTAGGCATTTCGGGAGGTCAGGATTCTA
CCCTCGCAGGAAAGCTAGCCCAAATGGCCATTGCAGAGTTACGGGAGGAAGCAAGTGATCAAGCTTACCAATTTATAGCT
GTTCGCTTGCCTTATGGGGTTCAGGCTGATGAAGCAGATGCGCAAAAAGCCTTGGCTTTTATTGCACCAGATCAGACCCT
TACCATTAATATCAAGGCAGCTGTGGACGGTCAAGTAGAGGCTCTACAAGCAGCAGGCGTTGAGATTTCTGATTTCAATA
AAGGTAATATTAAAGCACGTCAGCGAATGATCAGTCAATATGCCATTGCTGGCCAAATGGCTGGTGCTGTTATTGGAACG
GATCATGCGGCAGAAAACATCACTGGTTTCTTTACTAAATTTGGTGATGGTGGAGCAGATATTCTTCCCCTCTTTAGGCT
TAACAAACGTCAAGGAAAGGCTCTTTTGAAAGTTTTAGGAGCAGATGCTGCTTTGTATGAAAAAGTTCCAACTGCTGATT
TAGAAGATCAAAAACCAGGCTTGGCTGATGAGGTTGCTCTAGGGGTGACCTATCAAGACATCGACGACTATTTGGAAGGG
AAGCTGATTTCCAAAGTAGCTCAAGCAACTATTGAAAAATGGTGGCATAAAGGACAACACAAGCGTCACCTGCCAATCAC
CATTTTTGATGATTTTTGGAAATAA

Upstream 100 bases:

>100_bases
CCTCAAGACTACCCAGTAGACTTAGCCCGTGATGTGTGGCAAAACAAGATGGCCTTAATTGATAACATTCGCAAAGATGC
TTATGGAAAGAGTGAATGAC

Downstream 100 bases:

>100_bases
GAGATGATGTTCAAAGAGTAAAAAAAGAAAACAAAAAAAGTTAGGTTATCCTAACTTTTTTCTTGAAGAAAATCAAATAA
GGTCTATAATAGAAGTAATC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIA
VRLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK

Sequences:

>Translated_274_residues
MTLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIA
VRLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
>Mature_273_residues
TLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIAV
RLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTD
HAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEGK
LISKVAQATIEKWWHKGQHKRHLPITIFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=64.963503649635, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_STRP1 (Q99YK9)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269697.1
- RefSeq:   YP_282720.1
- ProteinModelPortal:   Q99YK9
- SMR:   Q99YK9
- EnsemblBacteria:   EBSTRT00000001303
- EnsemblBacteria:   EBSTRT00000027650
- GeneID:   3571550
- GeneID:   901905
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1652
- KEGG:   spz:M5005_Spy_1357
- GeneTree:   EBGT00050000027750
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   SPYO160490:SPY1652-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1357-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30075; Mature: 29943

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLA
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
QMAIAELREEASDQAYQFIAVRLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVE
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHEEEECCCCEEEEEEEEECCCHHH
ALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGAD
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
CHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHH
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
QMAIAELREEASDQAYQFIAVRLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVE
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHEEEECCCCEEEEEEEEECCCHHH
ALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGAD
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
CHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHH
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296