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The map label for this gene is murD

Identifier: 15675424

GI number: 15675424

Start: 1253543

End: 1254901

Strand: Reverse

Name: murD

Synonym: SPy_1525

Alternate gene names: 15675424

Gene position: 1254901-1253543 (Counterclockwise)

Preceding gene: 15675425

Following gene: 161378149

Centisome position: 67.74

GC content: 40.47

Gene sequence:

>1359_bases
ATGAAAGTGATAAGTAATTTTCAAAACAAAAAAATATTAATATTGGGGTTAGCCAAATCGGGCGAAGCAGCAGCAAAATT
ATTGACCAAACTTGGTGCTTTAGTGACTGTTAATGATAGTAAACCATTTGACCAAAATCCAGCGGCACAAGCCTTGTTGG
AAGAGGGGATTAAGGTCATTTGTGGTAGCCACCCAGTAGAATTATTAGATGAGAACTTTGAGTACATGGTTAAAAACCCT
GGGATTCCTTATGATAATCCTATGGTTAAACGCGCCCTTGCAAAGGAAATTCCCATCTTGACTGAAGTAGAATTGGCTTA
TTTCGTATCTGAAGCGCCTATTATCGGGATTACAGGATCAAACGGGAAGACAACCACAACGACAATGATTGCCGATGTTT
TGAATGCTGGCGGGCAATCTGCACTCTTATCTGGAAACATTGGTTATCCTGCTTCAAAAGTTGTTCAAAAAGCAATTGCT
GGTGATACTTTGGTGATGGAATTGTCCTCTTTTCAATTAGTGGGAGTGAATGCTTTTCGCCCTCATATTGCTGTCATCAC
TAATTTAATGCCGACTCACCTGGACTATCATGGCAGTTTTGAGGATTATGTTGCTGCTAAATGGATGATTCAAGCTCAGA
TGACAGAATCAGACTACCTTATTTTAAATGCTAATCAAGAGATTTCAGCAACTCTAGCTAAGACCACCAAAGCAACAGTG
ATTCCTTTTTCAACTCAAAAAGTGGTTGATGGAGCTTATCTGAAGGATGGAATACTCTATTTTAAAGAACAGGCGATTAT
AGCTGCAACTGACTTAGGTGTCCCAGGTAGCCACAACATTGAAAATGCCCTAGCAACTATTGCAGTTGCCAAGTTATCTG
GTATTGCTGATGATATTATTGCCCAGTGCCTTTCACATTTTGGAGGCGTTAAACATCGTTTGCAACGGGTTGGTCAAATC
AAAGATATTACCTTCTACAATGACAGTAAGTCAACCAATATTTTAGCCACTCAAAAAGCTTTATCAGGTTTTGATAACAG
TCGCTTGATTTTGATTGCTGGCGGTCTAGATCGTGGCAATGAATTTGACGATTTGGTGCCAGACCTTTTAGGACTTAAGC
AGATGATTATTTTGGGAGAATCCGCAGAGCGTATGAAGCGAGCTGCTAACAAAGCAGAGGTCTCTTATCTTGAAGCTAGA
AATGTGGCAGAAGCAACAGAGCTTGCTTTTAAGCTGGCCCAAACAGGCGATACTATCTTGCTTAGCCCAGCCAATGCTAG
CTGGGATATGTATCCTAATTTTGAGGTTCGTGGGGATGAATTTTTGGCAACCTTTGATTGTTTAAGAGGAGATGCCTAA

Upstream 100 bases:

>100_bases
ATTCTAATTCTAAACTCAAGAACGGTATTTTTAAAATGTTGAAAAAGCATCTAAATAAAGGTAAAATAGAAATCAAATAG
AATGGATAGTGTGAGCTTAG

Downstream 100 bases:

>100_bases
TGCCTAAGAAGATTTTATTTACAGGTGGTGGAACTGTAGGTCATGTCACCTTGAACCTCATTCTCATACCAAAATTTATC
AAGGACGGTTGGGAAGTACA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA

Sequences:

>Translated_452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA
>Mature_452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=446, Percent_Identity=29.5964125560538, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_STRP1 (P0C0D8)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269598.1
- RefSeq:   YP_282616.1
- ProteinModelPortal:   P0C0D8
- SMR:   P0C0D8
- EnsemblBacteria:   EBSTRT00000001592
- EnsemblBacteria:   EBSTRT00000028820
- GeneID:   3571643
- GeneID:   901569
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1525
- KEGG:   spz:M5005_Spy_1253
- GeneTree:   EBGT00050000027197
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   SPYO160490:SPY1525-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1253-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48728; Mature: 48728

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHEE
CGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGS
CCCCCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECC
NGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIAGDTLVMELSSFQLVGVNAFR
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCEEEEECCCCC
PHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHCCCEEE
IPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDII
EECCHHHHHCCHHHHCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGN
HHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEARNVAEATELAFKLAQTGDTIL
CHHHHHHHHHHHHHHEEECCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYPNFEVRGDEFLATFDCLRGDA
EECCCCCCCCCCCEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHEE
CGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGS
CCCCCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECC
NGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIAGDTLVMELSSFQLVGVNAFR
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCEEEEECCCCC
PHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHCCCEEE
IPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDII
EECCHHHHHCCHHHHCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGN
HHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEARNVAEATELAFKLAQTGDTIL
CHHHHHHHHHHHHHHEEECCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYPNFEVRGDEFLATFDCLRGDA
EECCCCCCCCCCCEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296