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The map label for this gene is rsuA [H]

Identifier: 15675276

GI number: 15675276

Start: 1108178

End: 1108912

Strand: Reverse

Name: rsuA [H]

Synonym: SPy_1337

Alternate gene names: 15675276

Gene position: 1108912-1108178 (Counterclockwise)

Preceding gene: 15675278

Following gene: 161378150

Centisome position: 59.86

GC content: 42.99

Gene sequence:

>735_bases
ATGATGCGCTTAGATAAATTTTTAGTGGCAACTGGTGTTGGAACTAGGAGCCAAGTCAAACTTCTCTTAAAAAAGAAGGC
TATTTTCGTCAATCAAAAGGTTGAAACCTCTGCCAAAGCACACATTGATGAATACAAGGATTTGGTGACGTATCAAGGTA
CGCCCTTGGTCTATGAATCCTTTGTTTATTACCTGTTAAACAAGCCGTCAGGGTATGTGTCAGCAACCCAGGACAGACAG
CAGGCTACTGTTATGGAGTTGTTAGATGACACGGCTAGGCAAAAAGCAGTTTTTCCTGTGGGTAGGTTAGATAAGGATAC
CCGTGGACTTTTGCTCTTGACCAATAATGGACAACTAGCCCATGACCTTTTATCCCCTAAAAAACATGTTACGAAAGAGT
ACTTGGCTAAAGTAGCAGGCATCATGACTGAAGCTGATAAAGATTATTTTGCTAGAGGTATTTCCTTAAAGGATCACCAG
TGCCTACCTGCTCATTTAGAAGTGCTTGCTAGTGATTTACAGCAGCAAACAAGTTTGGTTAAAATTACCATACAAGAAGG
AAAATTCCATCAGGTGAAACGCATGGTAGCAGCCTGTGGAAAGGAAGTGCTTGACTTACAGCGTCTCAGCATGGGACCAT
TAAAACTAGACCCTAGCCTTGCAGAAGGGGAGTTTAGGCGCCTGACGCCTGAGGAATTGCAGAGCCTGGCACCTTATTGT
CAGAGCTCACTATAA

Upstream 100 bases:

>100_bases
CTCGTTTCTATAAGCCTATTATAATATATTAAGCGCTTACAGTCATGAAACTACTGTTTTTTTATGTTATACTTAATAAT
GAATTTTCTTTGAAAGGGTT

Downstream 100 bases:

>100_bases
AAAGAAACCTCCCCGGTCAGTACTGACCCCAAAAAGTTGGACAAATAATTATTGAAAGGATTTAGTTCTGTATTGGACAG
GACTAAGTCCTTTTAGTTTT

Product: putative 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQ
QATVMELLDDTARQKAVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQ
CLPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYC
QSSL

Sequences:

>Translated_244_residues
MMRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQ
QATVMELLDDTARQKAVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQ
CLPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYC
QSSL
>Mature_244_residues
MMRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQ
QATVMELLDDTARQKAVFPVGRLDKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQ
CLPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYC
QSSL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=37.3949579831933, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.8300395256917, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081838, Length=148, Percent_Identity=38.5135135135135, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27352; Mature: 27352

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYES
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRLDKDTRGLLLLTNNGQLA
HHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHH
HDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQCLPAHLEVLASDLQQQTSLV
HHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEE
KITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYC
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
QSSL
HCCC
>Mature Secondary Structure
MMRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEYKDLVTYQGTPLVYES
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRLDKDTRGLLLLTNNGQLA
HHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHH
HDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQCLPAHLEVLASDLQQQTSLV
HHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEE
KITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPSLAEGEFRRLTPEELQSLAPYC
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
QSSL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]