Click here to switch to the map view.

The map label for this gene is truB

Identifier: 15675211

GI number: 15675211

Start: 1032007

End: 1032891

Strand: Reverse

Name: truB

Synonym: SPy_1251

Alternate gene names: 15675211

Gene position: 1032891-1032007 (Counterclockwise)

Preceding gene: 15675212

Following gene: 15675210

Centisome position: 55.76

GC content: 41.58

Gene sequence:

>885_bases
ATGATAAATGGAATCATTAATTTAAAAAAAGAAGCCGGCATGACCTCTCATGACGCAGTTTTTAAATTACGGAAGTTATT
ACAAGAAAAAAAGATTGGACATGGGGGGACCTTAGATCCTGATGTGGTCGGTGTTCTACCAATTGCAGTTGGTAAGGCTA
CTCGTGTGATCGAATACATGACAGAAGCTGGTAAAGTTTATGAAGGACAAGTGACTCTCGGTTACTCAACAACGACTGAG
GATGCTAGTGGAGAGGTAGTTGCGAGGTCCTCTTTGCCAGCTGTGCTAACAGAAGAACTCGTTGATCAAACGATGACGAC
TTTCCTTGGGAAAATCACACAGACCCCACCTATGTATTCAGCCGTAAAGGTTAATGGCCGTAAATTATACGAGTATGCGC
GTGCTGGTGAAAGTGTGGAACGTCCTAGAAGAGAAGTGACTATTTCTCTGTTTGAACGCACCAGCCCTTTGAATTTTACT
GAAGACGGTCTTTGCCGATTCTCTTTTAAAGTTGCTTGTAGCAAAGGGACTTATGTGCGGACCTTAGCAGTTGATTTAGG
TCGAGCTCTAGGGGTAGAGAGTCATATGTCCTTCTTGCAGCGATCAGCTTCAGCAGGATTAACCCTCGAGACTGCCTATA
CTTTGGGAGAAATTGCTGATATGGTTTCTAAACAAGAGATGTCTTTTTTGTTACCGATCGAGTATGGCGTTGCTGATCTT
CCTAAAATGGTTATTGATGATACTGAATTGACTGAAATTTCATTTGGTCGCCGTCTTAGTCTCCCTAGTCAAGAGCCACT
GTTAGCTGCTTTTCATGGCGAAAAAGTAATTGCTATTTTAGAAAAAAGAGATCAAGAATACAAACCCAAAAAAGTTCTTA
TTTAA

Upstream 100 bases:

>100_bases
TTTCCCTGCTAAAATTGGTTAAGCGTTTATTTTGGCAAAACTGATGATAAAGCGGTAGAATAGAATCAGTAACAATTACT
AATTAGAGAGAAAAATAGAC

Downstream 100 bases:

>100_bases
ATAGTTTGAGTAAGTAGAATGGATAATTATGGAAATTGAATATATCAAAGATTATCGTGATATTAACCAAGAAGATGATA
CAGTCTTGATATTGGGATAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTE
DASGEVVARSSLPAVLTEELVDQTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEIADMVSKQEMSFLLPIEYGVADL
PKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKVIAILEKRDQEYKPKKVLI

Sequences:

>Translated_294_residues
MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTE
DASGEVVARSSLPAVLTEELVDQTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEIADMVSKQEMSFLLPIEYGVADL
PKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKVIAILEKRDQEYKPKKVLI
>Mature_294_residues
MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTE
DASGEVVARSSLPAVLTEELVDQTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEIADMVSKQEMSFLLPIEYGVADL
PKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKVIAILEKRDQEYKPKKVLI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=237, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI4503337, Length=272, Percent_Identity=29.7794117647059, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI215599015, Length=272, Percent_Identity=29.7794117647059, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI2367200, Length=250, Percent_Identity=36, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323204, Length=271, Percent_Identity=28.4132841328413, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=41.5384615384615, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364189, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364187, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364185, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364183, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI62471759, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17975520, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_STRP1 (P65858)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269385.1
- RefSeq:   YP_282324.1
- ProteinModelPortal:   P65858
- SMR:   P65858
- EnsemblBacteria:   EBSTRT00000001633
- EnsemblBacteria:   EBSTRT00000027966
- GeneID:   3571936
- GeneID:   901351
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1251
- KEGG:   spz:M5005_Spy_0961
- GeneTree:   EBGT00050000027943
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK02484
- BioCyc:   SPYO160490:SPY1251-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0961-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32346; Mature: 32346

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: ACT_SITE 39-39

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYM
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVDQTMTTFLGKITQTPPMYS
HHCCCEEECEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
AVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFTEDGLCRFSFKVACSKGTYVR
EEEECCHHHHHHHHCCCHHHCCHHHEEHEEECCCCCCCCCCCCCEEEEEEEEECCCCEEE
TLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEIADMVSKQEMSFLLPIEYGVADL
HHHHHHHHHHCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHC
PKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKVIAILEKRDQEYKPKKVLI
CHHHCCCCCHHHCCCCCEECCCCCCCEEEEECCCEEEEEEHHCCCCCCCCCCCC
>Mature Secondary Structure
MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYM
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVDQTMTTFLGKITQTPPMYS
HHCCCEEECEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
AVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFTEDGLCRFSFKVACSKGTYVR
EEEECCHHHHHHHHCCCHHHCCHHHEEHEEECCCCCCCCCCCCCEEEEEEEEECCCCEEE
TLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEIADMVSKQEMSFLLPIEYGVADL
HHHHHHHHHHCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHC
PKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKVIAILEKRDQEYKPKKVLI
CHHHCCCCCHHHCCCCCEECCCCCCCEEEEECCCEEEEEEHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296