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The map label for this gene is trmE

Identifier: 15675063

GI number: 15675063

Start: 880855

End: 882231

Strand: Reverse

Name: trmE

Synonym: SPy_1071

Alternate gene names: 15675063

Gene position: 882231-880855 (Counterclockwise)

Preceding gene: 15675078

Following gene: 15675032

Centisome position: 47.63

GC content: 42.48

Gene sequence:

>1377_bases
ATGAGTATAACTAAAGAATTTGATACAATTACCGCCATTTCAACCCCTCTTGGTGAAGGAGCTATTGGCATTGTTCGTTT
ATCAGGGACAGATGCGCTTGCCATTGCACAATCTGTTTTTAAGGGAAAGAACTTAGAGCAAGTCGCCTCACACACCATTA
ACTATGGCCATATCATTAATCCTAAGACAGGAACTATTATCGATGAAGTTATGGTGTCAGTCATGCTAGCCCCCAAAACC
TTTACCCGTGAAAATGTGGTTGAAATCAATACGCACGGTGGTATTGCTGTGACAAATGAAATCTTGCAACTATTGATTCG
CCAAGGCGCTAGAATGGCAGAGCCTGGCGAATTCACCAAACGTGCCTTCTTAAATGGGCGAGTTGATTTGACTCAAGCTG
AGGCTGTCATGGACATTATTCGTGCCAAAACAGACAAGGCTATGACGATTGCAGTCAAGCAACTGGATGGCTCCCTTTCC
CAGCTTATCAATGACACCCGTCAGGAAATTCTCAACACCTTAGCTCAAGTCGAAGTCAATATTGACTACCCTGAATACGA
CGATGTGGAAGAAATGACCACTGCTCTGCTACGTGAGAAAACACAAGAATTCCAAAGCCTTTTGGAAAGTCTCCTTCGAA
CAGCTAAACGCGGTAAAATTCTGCGTGAGGGCTTATCAACAGCTATTATTGGTCGTCCAAACGTCGGTAAGTCAAGCTTA
CTTAATAATTTGTTGCGAGAAGACAAGGCTATTGTGACTGATATTGCAGGAACCACCCGAGATGTCATTGAAGAATACGT
TAATATCAAGGGAGTTCCTCTAAAACTTGTCGATACGGCCGGTATTCGTGAGACAGATGATCTGGTCGAACAAATCGGAG
TTGAACGGTCTAAAAAAGCTCTTCAAGAAGCTGACCTTGTTCTTCTTGTGCTGAATGCTTCTGAAAAACTGACTGACCAA
GACCGGGCTTTGTTAAATCTCAGTCAGGACAGTAACCGTATTATATTGTTGAATAAAACGGATTTGGAACAGAAGATTGA
ATTAGAGCAATTACCAGATGACTACATTCCCATTTCCGTTTTAACCAACCAGAACATTAATCTAATCGAAGATCGTATCA
ACCAGCTTTTCTTTGACAACGCTGGTTTGGTAGAGCAAGATGCCACTTACCTGTCAAATGCCCGCCACATTTCTTTGATT
GAAAAGGCAGTCCAGAGTTTAGAAGCTGTCAATGACGGTTTGGCGCTTGGCATGCCAGTTGATTTACTACAGGTTGATTT
AACCAGAACTTGGGAAATTCTCGGAGAAATCACAGGGGATGCTGCTCCTGATGAACTCATTACCCAACTCTTTAGTCAAT
TCTGTCTTGGTAAATAA

Upstream 100 bases:

>100_bases
TTATTTTTTTAGTATTAGCTAAACTCAAAATGGTGCTAGTCCTATTTTCTATACAAATAACACCTTGCTTTCTACTAGGA
CTTGATTTAAAATAGAGGGC

Downstream 100 bases:

>100_bases
CAACAGTCTCTAATCATCACAAAAGAGCTGGAACAGTGTTCCAGCTCTTTTTGCTGTCATAAGCATCTTATTTAATTAAT
TCGTAGATTGCTTCAGCATA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIINPKTGTIIDEVMVSVMLAPKT
FTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQ
DRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLI
EKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Sequences:

>Translated_458_residues
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIINPKTGTIIDEVMVSVMLAPKT
FTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQ
DRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLI
EKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
>Mature_457_residues
SITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIINPKTGTIIDEVMVSVMLAPKTF
TRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQ
LINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRGKILREGLSTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQD
RALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIE
KAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=32.762312633833, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=31.2625250501002, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=31.9474835886214, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=33.5497835497836, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082120, Length=126, Percent_Identity=42.0634920634921, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=30.3030303030303, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.4259634888438, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=29.4491525423729, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_STRP1 (Q99ZU0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269237.1
- RefSeq:   YP_282157.1
- ProteinModelPortal:   Q99ZU0
- SMR:   Q99ZU0
- EnsemblBacteria:   EBSTRT00000001531
- EnsemblBacteria:   EBSTRT00000028902
- GeneID:   3572120
- GeneID:   901199
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1071
- KEGG:   spz:M5005_Spy_0794
- GeneTree:   EBGT00050000027398
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   SPYO160490:SPY1071-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0794-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR003661
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- SMART:   SM00388
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50510; Mature: 50378

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 88-88 BINDING 127-127 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIIN
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
PKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTK
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLINDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTDQDRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISV
HHHCCEEEEEECCCCCCCCHHHHHEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
LTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIEKAVQSLEAVNDGLALGMPV
EECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIIN
CCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
PKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTK
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLINDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTDQDRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISV
HHHCCEEEEEECCCCCCCCHHHHHEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
LTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIEKAVQSLEAVNDGLALGMPV
EECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296