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The map label for this gene is amiC [H]

Identifier: 15674924

GI number: 15674924

Start: 746218

End: 747672

Strand: Direct

Name: amiC [H]

Synonym: SPy_0902

Alternate gene names: 15674924

Gene position: 746218-747672 (Clockwise)

Preceding gene: 15674923

Following gene: 15674925

Centisome position: 40.28

GC content: 42.2

Gene sequence:

>1455_bases
ATGACTTATCAAGATGCGACGGCTATGGCTATTGCTGTTCAGACTGGTCAGACCACACCACTAGAATTAGTAACCCAGGC
TATTTATAAGGCTAAAAAGTTGAACCCTACCTTAAATGCTATTACCTCAGAACGCTTTGAAGCAGCCCTAGAAGAAGCCA
AACAAAGGGATTTTTCAGGTCTGCCTTTTGCTGGTGTGCCTCTTTTTTTAAAAGATTTGGGACAAGAATTAAAAGGTCAC
TCATCAACCTCAGGTTCCAGATTGTTTAAAGAGTATCAAGCGACTAAAACGGATTTATTTGTGAAAAGATTAGAAGCTTT
AGGCTTTATTATTTTAGGGCGCTCCAACACGCCTGAATTTGGCTTTAAAAATATTAGCGATAGCAGTTTGCACGGACCAG
TGAATTTACCAAGGGATAATACACGAAATGCTGGTGGCTCATCTGGGGGAGCTGCTGCTCTAGTGTCATCAGGAATTAGT
GCGCTAGCAACTGCTAGTGATGGTGGTGGTTCTATTCGAATTCCAGCCTCGTTTAATGGCTTAATTGGGCTCAAACCTAG
CCGTGGGCGCATGCCTGTCGGACCTGGTTCTTATCGAAGCTGGCAAGGAGCCTCAGTTCATTTTGCCTTGACCAAATCCG
TTAGAGATACTAGAAATCTACTCTACTATTTACAGATGGAACAAATGGAAAGCCCCTTCCCCCTAGCCACCTTAACAAAA
GATAGTATTTATCAATCGCTACAACGTCCCTTGACAATTGCTTTTTATCAACGGTTAAGTGACGGTAGCCCAGTATCTTT
AGATACTGCAAAAGCTTTAAGACAAGCAGTAACTTGGCTAAGAGAACAAGGGCATCAGCTAGTTGAATTGGAAGAGTTTC
CTGTAAATATGACAGAAGTGATTCGTCATTATTATATTATGAATAGTGTGGAAACGGCTGCTATGTTTGCTGATATTGAA
GACACTTTTGGACGTCCGATGACCAAAGACGATATGGAAACCATGACCTGGGCTATTTACCAATCAGGTAAAGACATCCC
AGCTTGGCGTTACTCACAGGTCCTTCAAAAATGGGATACGTACAGTGCAACCATGGCTAGTTTTCATGAGACTTATGACC
TGTTGTTGACGTTCACAACCAACACACCAGCTCCTAAGCACGGAGAACTTGTCCCAGATTCGAAACTCATGGCTAACTTG
GCTCAAGCTGAAATTTTCAGCAGTGAAGAGCAATTCAATTTGGTCGAAACCATGTTTGGTAAGAGTCTGGCTATTAATCC
TTACACAGCTCTGCCTAATCTAACTGGCCAGCCAGCTATTAGTTTACCTACTTATGAAACTAAGGAAGGTCTCTCAATGG
GCATTCAACTAATTGCAGCCAAAGGTAGAGAGGACCTATTATTAGGAATTGCAGAACAGTTTGAGGCAGCAGGATTATTG
AAGATTCCTCAATAA

Upstream 100 bases:

>100_bases
CACATTCTAATTTCTAACCTTTTAATGGTGGTTTTCTTTTATTTGTGTTTTAATAAAAAAGAATGTTTTAGGATTCATAT
CTTAGAAAGGAAGAGTAGCT

Downstream 100 bases:

>100_bases
TAATCTTATAAAACAGTTTAACTATTATAGATTAACTTTTTAAAGAGATTGAGTGCTCACTGATAACACAACTTAATCAA
AATGTTATACTATTCTTATC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGH
SSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGIS
ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ

Sequences:

>Translated_484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGH
SSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGIS
ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ
>Mature_483_residues
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGHS
STSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGISA
LATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTKD
SIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIED
TFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLA
QAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQLIAAKGREDLLLGIAEQFEAAGLLK
IPQ

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=510, Percent_Identity=26.8627450980392, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI222831590, Length=526, Percent_Identity=23.574144486692, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=325, Percent_Identity=30.1538461538462, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=185, Percent_Identity=37.8378378378378, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=28.343949044586, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=297, Percent_Identity=29.96632996633, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=318, Percent_Identity=29.2452830188679, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=318, Percent_Identity=29.2452830188679, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=315, Percent_Identity=29.2063492063492, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=154, Percent_Identity=37.012987012987, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=247, Percent_Identity=28.3400809716599, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=469, Percent_Identity=24.3070362473348, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=202, Percent_Identity=28.7128712871287, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=26.171875, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=26.171875, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=26.171875, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=26.171875, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45550774, Length=196, Percent_Identity=37.7551020408163, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24644968, Length=526, Percent_Identity=25.6653992395437, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648435, Length=196, Percent_Identity=37.7551020408163, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648437, Length=196, Percent_Identity=37.7551020408163, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=29.3617021276596, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=26.4077669902913, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=127, Percent_Identity=41.7322834645669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648439, Length=127, Percent_Identity=41.7322834645669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648113, Length=186, Percent_Identity=34.4086021505376, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53115; Mature: 52984

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
LPFAGVPLFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEF
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGISALATASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCCCCCH
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC
>Mature Secondary Structure 
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
LPFAGVPLFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEF
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGISALATASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCCCCCH
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTFGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]