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The map label for this gene is thyA

Identifier: 15674907

GI number: 15674907

Start: 730241

End: 731080

Strand: Direct

Name: thyA

Synonym: SPy_0882

Alternate gene names: 15674907

Gene position: 730241-731080 (Clockwise)

Preceding gene: 15674904

Following gene: 15674908

Centisome position: 39.42

GC content: 34.88

Gene sequence:

>840_bases
ATGACAAAAGCTGACCAGATTTTTAAAGCTAATATTCAAAAAATTATAAACGAAGGATCATTGAGTGAGCAAGCACGTCC
TAAATATAAAGATGGTCGAACAGCTCATTCTAAGTATATTACTGGGGCGTTTGCTGAATATGATTTAGCTAAAGGAGAGT
TTCCCATCACAACTTTACGACCCATTCCAATTAAATCTGCTATAAAGGAATTATTTTGGATATATCAAGATCAGTCTAAC
AGTTTAGATGTGCTAGAAGCTAAATATAATGTTCATTATTGGAATGAATGGGAAGTTGACCAGACACGTACTATTGGGCA
ACGTTATGGTGCTGTAGTCAAAAAACATGATATCATCTCAAAAATACTAAAGCAATTAGCAGAAAATCCTTGGAATCGTC
GTAATGTCATTTCTCTCTGGGACTATGAAGCATTTGAGGAGACAAAAGGGTTGTTGCCATGTGCCTTTCAAATCATGTTT
GATGTTAGACGTGTAGGGGAAGATCTTTATTTAGATGCTAGTTTGACGCAACGTTCTAATGACATATTAGTTGCTCATCA
CATCAATGCCATGCAATACGTTGCCTTGCAGATGATGATTGCTAAACATTTTGGATGGAAAATAGGTAAGTTCTTTTATT
TTGTTAACAATTTACATATTTATGACAACCAATTTGATCAGGCTCAAGAATTGCTAAAACGACAGCCAGTTGCTAGTCAG
CCTAAGCTTGTTTTAAATGTTCCAGATAGGACTAATTTCTTTGATATTAAACCAGATGATTTTGAATTACAAAATTATGA
TCCAGTGAAGCCTCAGTTGCACTTTGACCTTGCTATTTAA

Upstream 100 bases:

>100_bases
CATGACCACATGAAGTTTTTCGAATGTTTATTTATTCTGGTATTTTTTGGTAAAATTAGTTAGAATAGTATGAAGTATGC
TTACAGAAAGGTTATTAGAG

Downstream 100 bases:

>100_bases
ACTTTATTTAGATTTTTTGTGAGCATTTTGTTCATAAATTTGGTAAAATAAATAGAGTTTAAAATGAAGGAAATAATTAA
TGACAAAGGAAATTATTGCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSN
SLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELLKRQPVASQ
PKLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFDLAI

Sequences:

>Translated_279_residues
MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSN
SLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELLKRQPVASQ
PKLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFDLAI
>Mature_278_residues
TKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNS
LDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMFD
VRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELLKRQPVASQP
KLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFDLAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=23.1316725978648, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789191, Length=266, Percent_Identity=30.0751879699248, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993377, Length=297, Percent_Identity=25.5892255892256, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324648, Length=168, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137556, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_STRP1 (P67051)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269081.1
- RefSeq:   YP_282051.1
- ProteinModelPortal:   P67051
- SMR:   P67051
- EnsemblBacteria:   EBSTRT00000000251
- EnsemblBacteria:   EBSTRT00000028742
- GeneID:   3572239
- GeneID:   901040
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0882
- KEGG:   spz:M5005_Spy_0688
- GeneTree:   EBGT00050000027007
- HOGENOM:   HBG588098
- OMA:   KSWDANA
- ProtClustDB:   PRK01827
- BioCyc:   SPYO160490:SPY0882-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0688-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32664; Mature: 32532

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLR
CCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECC
PIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIIS
CCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMFDVRRVGEDLYLDASLTQRSN
HHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECHHCCCCC
DILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELLKRQPVASQ
CEEEEECCCHHHHHHHHHHHHHHHCHHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCCC
PKLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFDLAI
CEEEEECCCCCCEEECCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
TKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLR
CCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECC
PIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIIS
CCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMFDVRRVGEDLYLDASLTQRSN
HHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECHHCCCCC
DILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELLKRQPVASQ
CEEEEECCCHHHHHHHHHHHHHHHCHHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCCC
PKLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFDLAI
CEEEEECCCCCCEEECCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296