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The map label for this gene is papS

Identifier: 15674894

GI number: 15674894

Start: 714037

End: 715230

Strand: Direct

Name: papS

Synonym: SPy_0866

Alternate gene names: 15674894

Gene position: 714037-715230 (Clockwise)

Preceding gene: 15674893

Following gene: 15674895

Centisome position: 38.55

GC content: 39.61

Gene sequence:

>1194_bases
ATGCCTTCTGAGTTTCAGAAGGCTTTACCCATATTGACTAAAATAAAAGAAGCTGGTTATGAAGCCTATTTTGTGGGAGG
AAGCGTCAGAGATGTTTTACTCGAGCGTCCCATTCATGATGTAGATATTGCAACTAGTTCCTATCCTGAAGAGACTAAAG
CGATTTTTAACAGAACTGTCGATGTTGGCATTGAACATGGTACAGTGCTCGTGCTTGAAAATGGCGGTGAGTATGAAATT
ACAACCTTTAGGACAGAAGATATCTATGTTGATTATCGCCGTCCTAGTCAGGTTTCTTTTGTGCGCTCTTTAGAGGAAGA
CTTAAAACGAAGAGACTTTACCGTCAATGCCTTGGCCTTGGATGAAAATGGTCAAGTTATTGATAAATTTAGAGGACTCA
TTGATTTAAAGCAAAAACGGTTACGAGCAGTGGGTAAAGCAGAGGAACGTTTTGAAGAAGATGCACTACGCATTATGAGA
GGCTTTCGATTTGCGGCGAGTCTGGATTTTGACATTGAAGCTATAACTTTTGAAGCTATGAGGAGCCATTCACCTCTATT
GGAAAAGATTTCTGTGGAACGTTCTTTTACTGAATTTGATAAATTATTGATGGCACCGCACTGGCGAAAAGGGATTTCTG
CCATGATTGCTTGTCAGGCTTACGACTATTTACCAGGCTTGAAGCAGCAGGAAGCAGGCTTGAATCATCTGATAGTTTCT
TTGAAAGATAACTTTACCTTTAGTGATTATCACCAAGCTTGGGCTTATGTGATGATTAGTTTAGCCATTGAGGATCCTAA
AAGCTTCCTAAAAGCATGGAAGACTTCAAATGATTTTCAGCGTTATGTCACAAAGTTGATTGCTCTTTATAGAATTCGTC
AGGAAAGATCTTTTGAAAAATTGGATATTTACCAGTACGGCAAGAAAATGGCGAGTCTAGTGGAAGACCTCCGAAAAGCT
CAAAGCTTATCCGTTGACATGGATCGTATTAATACTTTAGATCAGGCACTGGTTATTCATGACAAACACGATATCGTCCT
TAATGGTTCTCACCTGATTAAGGATTTTGGGATGAAATCAGGTCCTCAGCTAGGGCTTATGCTTGAGAAAGTTGAATTAG
CGATTGTAGAGGGGCGCCTAGATAATGATTTCACTACAATTGAAGCATTTGTTAGAGAGGAATTAGCAACATGA

Upstream 100 bases:

>100_bases
AAACAATAACAAAATGTGATAGAACCCAATTAGCCTAATCACACTTTAGATTGGGCTAGTTGGATGATAGGTTAGAAAAC
AACGTATGAAATTAATGACA

Downstream 100 bases:

>100_bases
GCCATTTTTTGGTGGAGAAATTAACAAAAACGGTGGGAGATAAGACCGTCTTTCAAGATATTTCCTTTATTATTCATGAC
TTTGACCGTATTGGGATTAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEI
TTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMR
GFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLNHLIVS
LKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKA
QSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT

Sequences:

>Translated_397_residues
MPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEI
TTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMR
GFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLNHLIVS
LKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKA
QSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
>Mature_396_residues
PSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEIT
TFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMRG
FRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLNHLIVSL
KDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKAQ
SLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=217, Percent_Identity=36.8663594470046, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI87081691, Length=211, Percent_Identity=35.5450236966825, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789436, Length=195, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=234, Percent_Identity=35.042735042735, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360112, Length=386, Percent_Identity=30.8290155440415, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21357337, Length=386, Percent_Identity=30.8290155440415, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_STRP1 (Q9A0A5)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269068.1
- RefSeq:   YP_282036.1
- ProteinModelPortal:   Q9A0A5
- SMR:   Q9A0A5
- EnsemblBacteria:   EBSTRT00000001513
- EnsemblBacteria:   EBSTRT00000028733
- GeneID:   3572224
- GeneID:   901027
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0866
- KEGG:   spz:M5005_Spy_0673
- GeneTree:   EBGT00050000028148
- HOGENOM:   HBG635063
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- BioCyc:   SPYO160490:SPY0866-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0673-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45630; Mature: 45499

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 35-35 BINDING 116-116 BINDING 159-159 BINDING 162-162 BINDING 165-165 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTV
CCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DVGIEHGTVLVLENGGEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALAL
EEEECCCEEEEEECCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAITFEAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
RSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLNHLIVS
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEE
LKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEK
EECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
LDIYQYGKKMASLVEDLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCEEECCHHHHHHCCCCC
GPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTV
CCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DVGIEHGTVLVLENGGEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALAL
EEEECCCEEEEEECCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAITFEAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
RSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLNHLIVS
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEE
LKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEK
EECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
LDIYQYGKKMASLVEDLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCEEECCHHHHHHCCCCC
GPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296