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The map label for this gene is trmD

Identifier: 15674882

GI number: 15674882

Start: 700525

End: 701256

Strand: Direct

Name: trmD

Synonym: SPy_0849

Alternate gene names: 15674882

Gene position: 700525-701256 (Clockwise)

Preceding gene: 15674881

Following gene: 15674883

Centisome position: 37.82

GC content: 40.44

Gene sequence:

>732_bases
ATGAAGATTGATATTTTAACCCTTTTTCCTGAAATGTTTGCCCCTCTAGAGCATTCCATTGTAGGCAAGGCAAAAGAAAA
AGGTCTGTTAGACATTCACTACCATAACTTTCGAGATTATGCTGAAAAAGCTCGTCATGTGGATGACGAACCCTATGGTG
GGGGCCAAGGCATGTTATTGAGAGCGCAGCCCATTTTTGACACCATTGAACAGATTGAAGCCAAAAAACCAAGGATTATT
CTTTTAGATCCAGCAGGTAAACCCTTTACTCAGGCTTATGCTGAAGAATTAGCTCTTGAGGAAGAGTTAATTTTTATTTG
TGGTCATTATGAAGGTTATGATGAGCGGATTAAGACCTTGGTGACGGATGAAATTTCATTAGGCGACTTTGTATTAACAG
GAGGTGAGTTGGCGGCCATGACTATGGTGGATGCGACCGTCAGGTTAATTCCACAGGTACTTGGGAAAGAAAGTAGCCAT
CAGGACGATTCTTTTTCATCAGGACTTTTAGAATACCCTCAATACACAAGACCTTATGATTATCGTGGTATGACAGTTCC
AGATGTTCTAATGAGTGGTCATCACGAACGTATTCGCTTGTGGCGTCTAGAAGAAAGTCTCAAAAAAACTTATTTAAGGC
GGCCAGATTTACTTGAACATTACAATTTTTCCGAGGAAGAGCGCAAGCTATTGGATAAGATTAAAGAAGCATTGGATCAA
GGAGAAGATTAG

Upstream 100 bases:

>100_bases
GGAAAACGAGACTTGCTCTTACCATATATTCCCCCAGTAGTTCTTAACGTAGATGTTCCTAACAAGCGCGTGGATGTTGA
ATTGATGGAAGGCTTAGACG

Downstream 100 bases:

>100_bases
TGAAAGATAAAGCATATGATATTACGATTATTGGTGGTGGTCCAATAGGTTTATTTGCAGCCTTTTATGCAGGTCTTAGG
GGCGTAACAGTAAAGATTAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLLEHYNFSEEERKLLDKIKEALDQ
GED

Sequences:

>Translated_243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLLEHYNFSEEERKLLDKIKEALDQ
GED
>Mature_243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLLEHYNFSEEERKLLDKIKEALDQ
GED

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=48.9626556016597, Blast_Score=222, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_STRP1 (Q9A0B6)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269056.1
- RefSeq:   YP_282019.1
- ProteinModelPortal:   Q9A0B6
- SMR:   Q9A0B6
- EnsemblBacteria:   EBSTRT00000001314
- EnsemblBacteria:   EBSTRT00000027695
- GeneID:   3572246
- GeneID:   901013
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0849
- KEGG:   spz:M5005_Spy_0656
- GeneTree:   EBGT00050000027798
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   SPYO160490:SPY0849-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0656-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27989; Mature: 27989

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLL
CEEEHEEHHHHHHCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTL
ECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLLEHYNFSEEERKLLDKIKEALDQ
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHC
GED
CCC
>Mature Secondary Structure
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLL
CEEEHEEHHHHHHCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTL
ECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLLEHYNFSEEERKLLDKIKEALDQ
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHC
GED
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296