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The map label for this gene is prfB [H]

Identifier: 15674713

GI number: 15674713

Start: 516178

End: 517191

Strand: Direct

Name: prfB [H]

Synonym: SPy_0643

Alternate gene names: 15674713

Gene position: 516178-517191 (Clockwise)

Preceding gene: 15674712

Following gene: 15674714

Centisome position: 27.86

GC content: 39.55

Gene sequence:

>1014_bases
TTGGAAGAGGAGATTGCTCTTCTTGAAAATCACATGACTGAGCCAGACTTTTGGAATGACAATATTGCAGCGCAAAAGAC
ATCTCAAGAACTAAATGAGTTAAAAGGTAAGTATGATACTTTCCATAATATGCAAGAATTATCTGACGAAACAGAACTCT
TACTTGAGATGCTTGACGAAGATGATTCTTTGAAAGAAGAATTAGAAGAAAATCTAATGCAGCTAGATAAGATAATGGGT
GCTTATGAAATGACGCTTCTCTTATCTGAACCCTATGACCACAATAATGCTATCTTAGAAATCCATCCTGGTTCAGGAGG
AACTGAAGCGCAAGATTGGGGAGATTTACTCTTACGAATGTATACCCGTTTTGGCAATGCTAATGGTTTCAAAATAGAAG
TATTGGATTATCAAGCAGGAGATGAAGCTGGTATTAAATCGGTTACCCTCTCTTTTGAAGGCCCCAATGCTTATGGTTTG
TTGAAATCAGAAATGGGAGTTCATCGCCTCGTTCGTATATCACCTTTTGATTCGGCTAAACGTCGTCATACCTCATTTGC
TTCAGTTGAAGTGATGCCAGAGTTAGATGATACGATTGAAGTAGAAGTACGTGATGATGACATTAAAATGGATACTTTCC
GTTCCGGAGGAGCTGGTGGTCAGAATGTCAATAAAGTATCAACAGGTGTTCGTTTGACGCATATTCCAACTGGAATTGTG
GTATCGTCTACCGTTGACCGTACTCAATATGGAAACCGCGATCGCGCTATGAAGATGTTGCAGGCTAAGCTATATCAGTT
AGAACAAGAAAAGAAAGCACAAGAAGTAGATGCTCTCAAGGGTGATAAAAAAGAAATCACTTGGGGAAGTCAGATTCGTT
CTTATGTTTTCACCCCTTATACGATGGTGAAGGATCATCGTACTAACTTTGAGCTTGCTCAGGTAGATAAGGTTATGGAT
GGAGAGATCAATGGCTTTATTGATGCCTACCTTAAGTGGCGCATTGAAGACTAA

Upstream 100 bases:

>100_bases
GATTGGAGAAATCTCATGGAAGTGGCTGAAATTCGCCAAAAAATAGTAGAAAATAAAGAGAAGTTGACTAGCTTCAGGAG
GTCTCTTTGACTTAGATCGT

Downstream 100 bases:

>100_bases
AAATAATAAAGGAGAAACATGGCACTAATAGAAATGTCTGGTGTTACTAAAAAATACCGCCGCTCAACGACCGCCCTCAG
AGACGTCAATGTTTCTGTTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQLDKIMG
AYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKIEVLDYQAGDEAGIKSVTLSFEGPNAYGL
LKSEMGVHRLVRISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMD
GEINGFIDAYLKWRIED

Sequences:

>Translated_337_residues
MEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQLDKIMG
AYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKIEVLDYQAGDEAGIKSVTLSFEGPNAYGL
LKSEMGVHRLVRISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMD
GEINGFIDAYLKWRIED
>Mature_337_residues
MEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQLDKIMG
AYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKIEVLDYQAGDEAGIKSVTLSFEGPNAYGL
LKSEMGVHRLVRISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMD
GEINGFIDAYLKWRIED

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=305, Percent_Identity=33.4426229508197, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI166795303, Length=282, Percent_Identity=34.7517730496454, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=34.7368421052632, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=45.3703703703704, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1787462, Length=315, Percent_Identity=33.968253968254, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=282, Percent_Identity=32.9787234042553, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=33.6477987421384, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI19921226, Length=278, Percent_Identity=32.0143884892086, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38381; Mature: 38381

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDE
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCC
DDSLKEELEENLMQLDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRM
CHHHHHHHHHHHHHHHHHHCHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
YTRFGNANGFKIEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAK
HHHHCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCCEEEEEECCCCHHH
RRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
HHHCCCEEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEE
VSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPY
EECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCH
TMVKDHRTNFELAQVDKVMDGEINGFIDAYLKWRIED
HEECCCCCCCHHHHHHHHHCCCHHHHEEEEEEEEECC
>Mature Secondary Structure
MEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDE
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCC
DDSLKEELEENLMQLDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRM
CHHHHHHHHHHHHHHHHHHCHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
YTRFGNANGFKIEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAK
HHHHCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCCEEEEEECCCCHHH
RRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
HHHCCCEEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEE
VSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPY
EECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCH
TMVKDHRTNFELAQVDKVMDGEINGFIDAYLKWRIED
HEECCCCCCCHHHHHHHHHCCCHHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA