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The map label for this gene is tuf [H]

Identifier: 15674691

GI number: 15674691

Start: 492202

End: 493398

Strand: Direct

Name: tuf [H]

Synonym: SPy_0611

Alternate gene names: 15674691

Gene position: 492202-493398 (Clockwise)

Preceding gene: 15674690

Following gene: 15674692

Centisome position: 26.57

GC content: 43.61

Gene sequence:

>1197_bases
ATGGCAAAAGAAAAATACGATCGTAGTAAACCCCACGTTAACATTGGTACAATCGGACACGTTGACCATGGTAAAACTAC
TTTAACAGCTGCAATCACAACTGTATTGGCACGTCGCTTGCCTTCATCAGTTAACCAACCAAAAGATTACGCTTCTATCG
ATGCTGCTCCAGAAGAACGCGAACGCGGAATCACTATCAACACTGCACACGTTGAGTACGAAACTGCAACTCGTCACTAT
GCGCACATCGACGCTCCAGGACACGCGGACTACGTTAAAAACATGATCACTGGTGCCGCTCAAATGGACGGAGCTATCCT
TGTAGTTGCTTCAACTGATGGACCAATGCCACAAACTCGTGAGCACATCCTTCTTTCACGTCAGGTTGGTGTTAAACACC
TTATCGTGTTCATGAACAAAGTTGACCTTGTTGATGACGAAGAGTTGCTTGAATTAGTTGAGATGGAAATTCGTGACCTT
CTTTCAGAATACGATTTCCCAGGTGATGACCTTCCAGTTATCCAAGGTTCAGCTCTTAAAGCTCTTGAAGGCGACACTAA
ATTTGAAGACATCATCATGGAATTGATGGATACTGTTGATTCATACATTCCAGAACCAGAACGCGACACTGACAAACCAT
TGCTTCTTCCAGTCGAAGACGTATTCTCAATTACAGGTCGTGGTACAGTTGCTTCAGGACGTATCGACCGTGGTACTGTT
CGTGTCAACGACGAAATCGAAATCGTTGGTATCAAAGAAGAAACTAAAAAAGCTGTTGTTACTGGTGTTGAAATGTTCCG
TAAACAACTTGACGAAGGTCTTGCAGGAGACAACGTAGGTATCCTTCTTCGTGGTGTTCAACGTGACGAAATCGAACGTG
GTCAAGTTATTGCTAAACCAAGTTCAATCAACCCACACACTAAATTCAAAGGTGAAGTATATATCCTTTCTAAAGACGAA
GGTGGACGTCACACTCCATTCTTCAACAACTACCGTCCACAATTCTACTTCCGTACAACTGACGTAACAGGTTCAATCGA
ACTTCCAGCAGGTACAGAAATGGTTATGCCTGGTGATAACGTGACAATCAACGTTGAGTTGATCCACCCAATCGCCGTAG
AACAAGGTACTACTTTCTCAATCCGTGAAGGTGGACGTACTGTTGGTTCAGGTATCGTTTCAGAAATCGAAGCTTAA

Upstream 100 bases:

>100_bases
TTGCATTTTCATTGTAATTTGCTACAATAGTAAGGTAAAGTTAGACTGTATGGCCTACCGTCTATCTATAAAATATATTT
TATTGGAGGCTTTTCCTAAA

Downstream 100 bases:

>100_bases
TTTTAATTAAGTTCCCAGAATAGCAATTTAAGTCAGACAACTTAAGTTTGAAGAAGCCCCCAATTCGGGGGCTTTTTGCT
ATCTTAAACTCCATAAAATG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETATRHY
AHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGTV
RVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAGDNVGILLRGVQRDEIERGQVIAKPSSINPHTKFKGEVYILSKDE
GGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGIVSEIEA

Sequences:

>Translated_398_residues
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETATRHY
AHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGTV
RVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAGDNVGILLRGVQRDEIERGQVIAKPSSINPHTKFKGEVYILSKDE
GGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGIVSEIEA
>Mature_397_residues
AKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVEYETATRHYA
HIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLL
SEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGTVR
VNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAGDNVGILLRGVQRDEIERGQVIAKPSSINPHTKFKGEVYILSKDEG
GRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGIVSEIEA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.4430379746835, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.7946428571429, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.5714285714286, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.2517006802721, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=29.2517006802721, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=321, Percent_Identity=29.9065420560748, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=321, Percent_Identity=29.595015576324, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018520, Length=321, Percent_Identity=29.595015576324, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=29.5731707317073, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=27.1028037383178, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=28.1481481481481, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157426893, Length=294, Percent_Identity=28.2312925170068, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=31.1594202898551, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=72.979797979798, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=72.979797979798, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=336, Percent_Identity=28.5714285714286, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789108, Length=254, Percent_Identity=32.6771653543307, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=30.3249097472924, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788922, Length=336, Percent_Identity=26.1904761904762, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=49.0049751243781, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25141371, Length=392, Percent_Identity=43.1122448979592, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.3571428571429, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.3571428571429, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.4382284382284, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=431, Percent_Identity=27.8422273781903, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=27.8422273781903, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=340, Percent_Identity=29.4117647058824, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=303, Percent_Identity=29.3729372937294, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=41.5584415584416, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.9722921914358, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=29.3064876957494, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=29.3064876957494, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=347, Percent_Identity=25.3602305475504, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=349, Percent_Identity=28.080229226361, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=143, Percent_Identity=32.1678321678322, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=55.5837563451777, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=55.5837563451777, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=47.4358974358974, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.6875, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.6875, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI45550900, Length=443, Percent_Identity=28.6681715575621, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=30.2931596091205, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=28.8629737609329, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647048, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647046, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43856; Mature: 43725

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEER
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHH
ERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTR
HCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCH
EHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLLSEYDFPGDDLPVIQGSALK
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHH
ALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGTV
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCCCCCEE
RVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAGDNVGILLRGVQRDEIERGQVIAKP
EECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCCCCCEEEECC
SSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDN
CCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCC
VTINVELIHPIAVEQGTTFSIREGGRTVGSGIVSEIEA
EEEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEER
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHH
ERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTR
HCCCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCH
EHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLLSEYDFPGDDLPVIQGSALK
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHH
ALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPVEDVFSITGRGTVASGRIDRGTV
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCCCCCEE
RVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAGDNVGILLRGVQRDEIERGQVIAKP
EECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCCCCCEEEECC
SSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDN
CCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCC
VTINVELIHPIAVEQGTTFSIREGGRTVGSGIVSEIEA
EEEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA