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The map label for this gene is smpB

Identifier: 15674609

GI number: 15674609

Start: 406207

End: 406674

Strand: Direct

Name: smpB

Synonym: SPy_0504

Alternate gene names: 15674609

Gene position: 406207-406674 (Clockwise)

Preceding gene: 15674608

Following gene: 15674610

Centisome position: 21.93

GC content: 40.81

Gene sequence:

>468_bases
ATGGCAAAAGGTGAAGGTCATATACTGGCGCAAAATAAAAAGGCTAGACATGATTATCACATTGTTGAAACCGTTGAAGC
AGGGATTGTCTTAACGGGAACTGAGATTAAAAGTGTTCGTGCAGCTCGTATTCAACTGAAAGATGGCTTTGCCCAAATTA
AGAATGGGGAAGCGTGGTTGGTCAACGTGCATATTGCACCTTTTGAACAAGGTAATATTTGGAACGCTGATCCTGAACGA
ACCCGCAAACTGCTGCTCAAAAAACGTGAGATTACGCATCTAGCAAATGAACTTAAAGGATCTGGCATGACCTTAGTTCC
TCTCAAAGTTTACCTCAAGGATGGCTTTGCTAAAGTTCTGATTGGTCTAGCTAAAGGGAAACACGAGTATGACAAACGCG
AAACCATTAAACGCCGTGATCAGGAAAGAGATATTAAAAAACAAATGAAACACTACAATGCAAGGTAA

Upstream 100 bases:

>100_bases
AAGTAGCCAAGGCAAAAACCAAGGGTAAAACTAAAAAAGGGTCTAAAAAACCATTTTACAAAGAACAAGCAAAAAAGAAG
AGTCGTAAAAGGAGTTAACT

Downstream 100 bases:

>100_bases
GGTGAATGAGTATTATGATAACTAAAGGACAAGTTCGTTTGTTACGAACTTATTCTTATGATAGCAACTTATACACGCAG
GGATTAGAACAACTCAACAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPER
TRKLLLKKREITHLANELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR

Sequences:

>Translated_155_residues
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPER
TRKLLLKKREITHLANELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
>Mature_154_residues
AKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERT
RKLLLKKREITHLANELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=42.3611111111111, Blast_Score=116, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_STRP1 (Q9A126)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268783.1
- RefSeq:   YP_281778.1
- ProteinModelPortal:   Q9A126
- SMR:   Q9A126
- EnsemblBacteria:   EBSTRT00000000170
- EnsemblBacteria:   EBSTRT00000027553
- GeneID:   3572509
- GeneID:   900733
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0504
- KEGG:   spz:M5005_Spy_0415
- GeneTree:   EBGT00050000027179
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   SPYO160490:SPY0504-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0415-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17730; Mature: 17599

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWL
CCCCCCCEEECCCCCCCCHHHEEECCCCEEEECCHHHHHHHHHEEECCCHHEECCCCEEE
VNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSGMTLVPLKVYLKDGFAKVL
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEHHCCHHHHH
IGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWL
CCCCCCEEECCCCCCCCHHHEEECCCCEEEECCHHHHHHHHHEEECCCHHEECCCCEEE
VNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSGMTLVPLKVYLKDGFAKVL
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEHHCCHHHHH
IGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296