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The map label for this gene is holB [H]

Identifier: 15674540

GI number: 15674540

Start: 337062

End: 337937

Strand: Direct

Name: holB [H]

Synonym: SPy_0400

Alternate gene names: 15674540

Gene position: 337062-337937 (Clockwise)

Preceding gene: 15674539

Following gene: 15674541

Centisome position: 18.2

GC content: 39.16

Gene sequence:

>876_bases
ATGGATTTAGCGCAAAAAGCTCCTAACGTTTATCAAGCTTTTCAGACAATTTTAAAGAAAGACCGTCTGAATCATGCTTA
TCTTTTTTCGGGTGATTTTGCTAATGAAGAAATGGCTCTTTTTTTAGCTAAGGTCATCTTTTGTGAACAGAAAAAGGATC
AGACGCCCTGCGGGCATTGTCGCTCTTGTCAATTGATTGAACAAGGAGATTTTGCCGATGTGACGGTATTGGAACCAACA
GGGCAAGTGATTAAAACGGATGTGGTCAAAGAAATGATGGCTAACTTTTCTCAGACAGGATATGAAAACAAACGACAAGT
TTTTATTATCAAAGATTGTGACAAAATGCATATCAATGCCGCTAATAGCTTGCTAAAATACATTGAGGAGCCTCAGGGAG
AAGCTTACATATTTTTATTGACCAATGATGATAACAAAGTGCTTCCGACCATTAAAAGTCGGACACAGGTTTTTCAGTTT
CCTAAAAACGAAGCCTATCTTTACCAATTGGCACAAGAAAAGGGATTATTAAACCATCAGGCTAAGCTAGTAGCCAAACT
TGCCACAAACACCAGTCATCTAGAACGTCTGTTGCAAACGAGCAAGCTTTTAGAACTGATAACTCAAGCAGAGCGTTTTG
TATCTATTTGGCTGAAAGATCAGTTGCAGGCATATTTAGCGTTGAACCGTCTGGTACAGTTAGCAACTGAAAAAGAAGAA
CAAGATTTAGTTTTGACCCTTTTGACCTTGCTCTTGGCAAGAGAGCGTGCGCAAACGCCTTTGACACAATTGGAGGCTGT
CTATCAGGCTAGGCTCATGTGGCAGAGCAATGTTAATTTTCAAAACACATTAGAATATATGGTGATGTCAGAATAA

Upstream 100 bases:

>100_bases
TTGTCACAATTGATGCGACTAAACCATTGAAAGAAGTTGTCTCTGTCGCTTTGGAACATGTCTTAGCCTTGCTTTTAGCC
TAGGATAAGGGAGGGTAGGA

Downstream 100 bases:

>100_bases
GTGCAATTGGAATTCGTTATGGGGAAACGAAGCTTGTCAAAAGACTTGCCCGTAGCGACCAAAGTAGCGGCGCATAAGGG
CAAAAAAGCTTTTGAAGAAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNTLEYMVMSE

Sequences:

>Translated_291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNTLEYMVMSE
>Mature_291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNTLEYMVMSE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=154, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787341, Length=153, Percent_Identity=28.7581699346405, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33506; Mature: 33506

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHC
CCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHINA
CCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
ANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQFPKNEAYLYQLAQEKGLLNHQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
AKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNTLEYMVMSE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECC
>Mature Secondary Structure
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHC
CCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHINA
CCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
ANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQFPKNEAYLYQLAQEKGLLNHQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
AKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNTLEYMVMSE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]