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The map label for this gene is tmk

Identifier: 15674539

GI number: 15674539

Start: 336409

End: 337044

Strand: Direct

Name: tmk

Synonym: SPy_0399

Alternate gene names: 15674539

Gene position: 336409-337044 (Clockwise)

Preceding gene: 15674538

Following gene: 15674540

Centisome position: 18.16

GC content: 41.98

Gene sequence:

>636_bases
ATGATCACAGGAAAACTCATAACCGTTGAAGGTCCAGATGGCGCAGGGAAAACGACTGTTTTAGAGCAACTCATTCCACT
GTTAAAACAAAAAGTGGCTCAGGATATCTTGACAACAAGAGAGCCTGGAGGTGTTGCCATTTCAGAACACATTAGAGAAC
TTATTTTAGACATCAACCATACTGCTATGGACCCTAAAACAGAACTCTTACTTTATATTGCGGCTCGAAGGCAGCATTTG
GTGGAAAAGGTACTACCAGCTCTTGAAGCAGGCCAGTTGGTTTTTATTGATCGTTTCATCGATAGTTCAGTAGCTTATCA
AGGTGCGGGACGCGGTTTGATCAAAGCAGACATTCAGTGGCTCAACGAGTTCGCAACAGATGGTTTAGAGCCTGACTTGA
CCCTTTATTTTGATGTTCCTTCTGAAATTGGTTTAGCACGGATTAACGCGAACCAACAAAGAGAAGTCAATCGTTTAGAT
TTGGAAACAATAGAGATACATCAACGAGTACGAAAAGGATACCTAGCCTTAGCTAAGGAGCATCCAAAACGTATTGTCAC
AATTGATGCGACTAAACCATTGAAAGAAGTTGTCTCTGTCGCTTTGGAACATGTCTTAGCCTTGCTTTTAGCCTAG

Upstream 100 bases:

>100_bases
GGATAGGATTGGTCCTAAAATAGAGCACTGCAAAGATATGCCAGTGCTTTTTAGCTCATTTTTTGATAAAATAGAACTAC
TAAAAAGTGAAGGAAATAAG

Downstream 100 bases:

>100_bases
GATAAGGGAGGGTAGGAATGGATTTAGCGCAAAAAGCTCCTAACGTTTATCAAGCTTTTCAGACAATTTTAAAGAAAGAC
CGTCTGAATCATGCTTATCT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGGVAISEHIRELILDINHTAMDPKTELLLYIAARRQHL
VEKVLPALEAGQLVFIDRFIDSSVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA

Sequences:

>Translated_211_residues
MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGGVAISEHIRELILDINHTAMDPKTELLLYIAARRQHL
VEKVLPALEAGQLVFIDRFIDSSVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA
>Mature_211_residues
MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGGVAISEHIRELILDINHTAMDPKTELLLYIAARRQHL
VEKVLPALEAGQLVFIDRFIDSSVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=198, Percent_Identity=42.4242424242424, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_STRP1 (Q9A190)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268713.1
- RefSeq:   YP_281694.1
- ProteinModelPortal:   Q9A190
- SMR:   Q9A190
- EnsemblBacteria:   EBSTRT00000000496
- EnsemblBacteria:   EBSTRT00000028281
- GeneID:   3572581
- GeneID:   900662
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0399
- KEGG:   spz:M5005_Spy_0330
- GeneTree:   EBGT00050000028362
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- BioCyc:   SPYO160490:SPY0399-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0330-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23403; Mature: 23403

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGGVAISEHIRELILDINH
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
TAMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDSSVAYQGAGRGLIKADIQW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHH
LNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLDLETIEIHQRVRKGYLALAKE
HHHHHHCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPKRIVTIDATKPLKEVVSVALEHVLALLLA
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGGVAISEHIRELILDINH
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
TAMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDSSVAYQGAGRGLIKADIQW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHH
LNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLDLETIEIHQRVRKGYLALAKE
HHHHHHCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPKRIVTIDATKPLKEVVSVALEHVLALLLA
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296