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The map label for this gene is yibK [C]

Identifier: 15674520

GI number: 15674520

Start: 318529

End: 319077

Strand: Direct

Name: yibK [C]

Synonym: SPy_0371

Alternate gene names: 15674520

Gene position: 318529-319077 (Clockwise)

Preceding gene: 15674519

Following gene: 15674521

Centisome position: 17.2

GC content: 37.34

Gene sequence:

>549_bases
ATGACGACAAAAGAACTTATCAACAAAAACGACAAAGTAAAAAAGGCTCGCAATCATATTGTCCTGTTTCAACCCCAAAT
TCCACAAAATACTGGTAATATTGCTAGAACATGTGCTGCAACTAATGCACCTTTACATATTATTAAACCCATGGGATTTC
CTATTGATGACCGCAAAATGAAGCGTGCAGGCTTGGATTACTGGGACAAGTTAGAACTGCATTTTTATGATCATTTAGAA
CAGTTTATCAATCAATGTCACGGACAGCTTCACCTGATTAGCAAGTTTGCAGTTAATAATTATTCTCAAGCTACTTATGC
TGATGGCGATTCCCATTATTTCTTGTTTGGACGAGAAGATACAGGCCTGCCAGAAGATTTTATGCGAGAACATGCTGAAA
AAGCACTTCGTATTCCGATGAATGATGAGCATGTACGGAGTTTAAATGTCTCAAATACGGTTTGTATGGTGATTTATGAA
GCCCTTAGACAACAGGGCTTTCAAGGGTTAGAGTTAAAGCATACTTACGAACACGATAAACTAAAATAA

Upstream 100 bases:

>100_bases
CTGTTATGATTTAAGTTAGCGAGTCACCATCAACCACATTGTTTGCTGAGCATGACTCCGAGCAGTGTGGTTTTTTAAAT
TAAAAAGAGTAAATGAGATT

Downstream 100 bases:

>100_bases
TGGACTTTTTAAAAGCGTCAGTTTTTGGCAACATGCGTTGTCAGTAGCTGACGCTTTTAAAAATTTAGTCAAAGCTATTG
CTATCTAGGTTTAAATCTGC

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLE
QFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
ALRQQGFQGLELKHTYEHDKLK

Sequences:

>Translated_182_residues
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLE
QFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
ALRQQGFQGLELKHTYEHDKLK
>Mature_181_residues
TTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQ
FINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEA
LRQQGFQGLELKHTYEHDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=42.6751592356688, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRML_STRPM (Q48V41)

Other databases:

- EMBL:   CP000056
- RefSeq:   YP_279770.1
- ProteinModelPortal:   Q48V41
- EnsemblBacteria:   EBSTRT00000031280
- GeneID:   3573253
- GenomeReviews:   CP000056_GR
- KEGG:   spb:M28_Spy0301
- GeneTree:   EBGT00050000027371
- HOGENOM:   HBG309728
- OMA:   EAWRQNG
- ProtClustDB:   CLSK877041
- BioCyc:   SPYO319701:M28_SPY0301-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01885
- InterPro:   IPR016914
- InterPro:   IPR001537
- PIRSF:   PIRSF029256

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 21182; Mature: 21051

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: BINDING 93-93 BINDING 117-117 BINDING 138-138 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKM
CCCHHHHCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
KRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGRED
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCC
TGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEALRQQGFQGLELKHTYEHDK
CCCCHHHHHHHHHHHEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
LK
CC
>Mature Secondary Structure 
TTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKM
CCHHHHCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
KRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGRED
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCC
TGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEALRQQGFQGLELKHTYEHDK
CCCCHHHHHHHHHHHEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA