Click here to switch to the map view.

The map label for this gene is araD [H]

Identifier: 15674384

GI number: 15674384

Start: 163947

End: 164651

Strand: Direct

Name: araD [H]

Synonym: SPy_0179

Alternate gene names: 15674384

Gene position: 163947-164651 (Clockwise)

Preceding gene: 15674383

Following gene: 15674385

Centisome position: 8.85

GC content: 46.95

Gene sequence:

>705_bases
ATGGCGAAAAACTTGCAAGAAATGCGTGAACGTGTCTGTGCGGCTAACAAGTCTCTACCACAACACGGTCTTGTCAAATT
TACGTGGGGCAATGTCTCTGAAGTTTGTCGTGAATTAGGACGTATTGTCATCAAGCCTTCTGGGGTGGACTACGACCTCT
TGACACCAGAAAATATGGTTGTTACAGACCTTGATGGGAATGTGGTCGAAGGTGATTTGAACCCTTCTTCAGATCTGCCA
ACCCATGTGGAACTTTATAAAGCTTGGCCAGAAGTGGGCGGTATTGTCCACACTCACTCCACAGAAGCTGTTGGTTGGGC
ACAGGCAGGTCGTGATATTCCGTTTTATGGGACTACGCATGCTGACTACTTCTATGGACCAGTTCCGTGTGCTCGTTCTC
TCACAAAAGCAGAAGTTGATGGGGCTTACGAGCAAGAAACTGGCAACGTGATTTTGGAAGAATTTAGCAAGCGAGGCTTA
GATCCTATGGCGGTTCCAGGAATCGTGGTTCGCAATCATGGCCCATTTACGTGGGGGAAAACCCCAGAGCAAGCCGTTTA
CCACTCGGTTGTCTTAGAAGAAGTGGCTAGGATGAATCGCTTGACAGAACAAATCAATCCACGCGTAGAGCCAGCGCCAA
GATACATTATGGATAAGCATTATTTGCGTAAACATGGCCCAAATGCTTATTACGGTCAAAAATGA

Upstream 100 bases:

>100_bases
TGGTCAGAAAATTGTGACACGGTGGAGGAAACAAAAGCTGCTATCAAAGAAGCTCAGGACTTCCTTTACCCACTTATTGA
GAAAGCGGGGTTGACATAAT

Downstream 100 bases:

>100_bases
GGTCGCGATATGGCTTATTAGCACAGGTTCTCACTAGAAGCCAGAAAGTTCAAGGTTTTGTTAATCATTGTTTGGGCAAG
TCCTTTTGACTTGTCCTTTT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: Phosphoribulose isomerase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVIKPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLP
THVELYKAWPEVGGIVHTHSTEAVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMDKHYLRKHGPNAYYGQK

Sequences:

>Translated_234_residues
MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVIKPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLP
THVELYKAWPEVGGIVHTHSTEAVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMDKHYLRKHGPNAYYGQK
>Mature_233_residues
AKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVIKPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPT
HVELYKAWPEVGGIVHTHSTEAVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGLD
PMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMDKHYLRKHGPNAYYGQK

Specific function: L-arabinose catabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=56.5217391304348, Blast_Score=278, Evalue=1e-76,
Organism=Escherichia coli, GI1790642, Length=230, Percent_Identity=54.7826086956522, Blast_Score=273, Evalue=8e-75,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=53.4782608695652, Blast_Score=267, Evalue=5e-73,

Paralogues:

None

Copy number: 136 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR004661 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 26158; Mature: 26027

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVIKPSGVDYDLLTPENMV
CCCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCCEEECCCCCCEEEECCCCEE
VTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTEAVGWAQAGRDIPFYGTTH
EEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCCC
ADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGLDPMAVPGIVVRNHGPFTWGK
CCCEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
TPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMDKHYLRKHGPNAYYGQK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVIKPSGVDYDLLTPENMV
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCCEEECCCCCCEEEECCCCEE
VTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTEAVGWAQAGRDIPFYGTTH
EEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCCC
ADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGLDPMAVPGIVVRNHGPFTWGK
CCCEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
TPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMDKHYLRKHGPNAYYGQK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA