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The map label for this gene is gidA

Identifier: 15673829

GI number: 15673829

Start: 1914418

End: 1916295

Strand: Reverse

Name: gidA

Synonym: L97777

Alternate gene names: 15673829

Gene position: 1916295-1914418 (Counterclockwise)

Preceding gene: 15673830

Following gene: 15673828

Centisome position: 81.01

GC content: 39.3

Gene sequence:

>1878_bases
ATGAATTTTCAAGAAAATTATGATGTTGTTGTCATTGGAGGAGGACATGCAGGGGTTGAAGCTTCACTAGCAGCTGCACG
GATGGGCTCTAAAACATTACTCATGACGATTAATCTTAATATGGTTGCCTTTATGCCATGTAATCCATCAATTGGTGGCT
CAGCTAAAGGAATTGTTGTCAGAGAAATTGATGCGCTCGGTGGTGAAATGGGTCGCAACATTGATAAGACTTATATTCAA
ATGAAAATGCTTAATACCGGAAAAGGTCCAGCAGTTCGAGCTCTTCGTGCTCAAGCCGATAAAGATGAATATGCCGATTC
AATGAAGAATACAGTTTCTGACCAAGAAAATTTGACACTCCGTCAAGGAATGGTTGAAGAACTTATTCTTGATGAAGAAA
AGAAAAAAGTCATCGGAATCAAGACTTCGACAGGAACTAAATATGGTGCTAAGGCAGTAATTATCACTACAGGAACAGCT
TTGCGTGGTGAAATTATTATTGGAGAACTTAAATATTCTTCAGGACCCAATAACTCTCTTTCATCAATTGGTCTAGCAGA
TAATTTACGTGAAATTGGATTTGAAATTGGTCGTTTTAAAACCGGAACTCCTCCACGCGTTTTGGCAAGTTCTATTGATT
ACGACAAAACAGAAATTCAACCAGGTGATGAAGCGCCTAATCATTTTAGTTTTATGAGCTCTGATGAAAATTATCTAAAA
GATCAAATTCCATGCTGGCTCACTTATACTACTGAGAATAGTCATACTATTTTGCGTGGTAATTTACACCGAGCACCACT
CTTTTCAGGAATTGTTAAAGGAGTAGGGCCACGTTACTGTCCTTCAATAGAAGATAAAATCACTCGCTTTGCTGACAAAC
CTCGTCATCAACTCTTCCTTGAACCAGAAGGACGAAATACAGAAGAAGTCTATATCGGTGGACTTTCAACTTCAATGCCT
GAAGATGTCCAATTTGATTTAGTTAAATCAATTCCAGGACTTGAAAATGCTCAAATGATGCGTCCGGGTTATGCTATAGA
ATACGATGTGGTGATGCCTCATCAACTCCGTCCAACACTTGAAACAAAATTGGTTTCTGGACTATTTACAGCGGGACAAA
CCAACGGGACCTCAGGCTATGAAGAAGCAGCAGGGCAAGGCTTAGTAGCTGGAATAAATGCAGCACTTAAAGTTCAAGGA
AAGTCTGAATTTATCTTAAAACGTAGTGAAGCCTATATTGGGGTAATGATTGATGATTTGGTAACTAAGGGAACGCTGGA
ACCATATCGTCTTTTGACATCACGTGCAGAATACCGTTTAATTCTTCGCCATGATAATGCTGACCGCCGTTTGACTGAAA
TTGGCCGTCAAGTGGGCCTTGTTTCAGATGAGCAATGGGAACATTACCAAGCAAAAATGGCTCAATTTGACCGTGAAATG
AAACGATTGAATTCAGAAAAACTCAAACCTTTGCCTGACACACAAGAAAAATTAGGAAAATTGGGCTTTGGACCAATTAA
AGATGCGCTGACAGGTGCAGAATTTTTGAAACGACCTGAAGTTCACTACAATGAAGTCATCGATTTTATCGGACAAGCAC
CAGAAAAGATTGACCGTACTGTCATTGAATTGATTGAAACTGAAATTACTTATGAAGGCTACATCAAAAAAGCAATGGAC
CAAGTGGACAAAATGCATCGCTTGGAAGCGAAACGAATTCCTAAAAATATGGATTGGGATAAGCTTGACTCAATTGCTAC
TGAAGCCCGTCAAAAATTCAAAAAAATAAATCCAGAAACGCTGGGACAAGCGAGTCGGATTTCAGGGGTAAATCCTGCTG
ACATCAGTATTCTCATGGTTTACCTTGAAGGAAAATAA

Upstream 100 bases:

>100_bases
AAGCTGTCAGTAAAAGAATGATTAATAACTCTGTCAGTATTTTGCAAGAACTTTTATCAAAATATAAAGAAAGGCGAATT
TAAAAAATTCGTAGTAAAAA

Downstream 100 bases:

>100_bases
TTAAAAATTAAAATTTTACTGACAAAAAGTTACTGACAGAAAATTTTTGGGAGAGTCCTTGTGTTTTGGCATTGAGGAAG
CAACTTAAAATTTTCGTCAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNFQENYDVVVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYADSMKNTVSDQENLTLRQGMVEELILDEEKKKVIGIKTSTGTKYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDENYLK
DQIPCWLTYTTENSHTILRGNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAQMMRPGYAIEYDVVMPHQLRPTLETKLVSGLFTAGQTNGTSGYEEAAGQGLVAGINAALKVQG
KSEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDEQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVHYNEVIDFIGQAPEKIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK

Sequences:

>Translated_625_residues
MNFQENYDVVVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYADSMKNTVSDQENLTLRQGMVEELILDEEKKKVIGIKTSTGTKYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDENYLK
DQIPCWLTYTTENSHTILRGNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAQMMRPGYAIEYDVVMPHQLRPTLETKLVSGLFTAGQTNGTSGYEEAAGQGLVAGINAALKVQG
KSEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDEQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVHYNEVIDFIGQAPEKIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK
>Mature_625_residues
MNFQENYDVVVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQ
MKMLNTGKGPAVRALRAQADKDEYADSMKNTVSDQENLTLRQGMVEELILDEEKKKVIGIKTSTGTKYGAKAVIITTGTA
LRGEIIIGELKYSSGPNNSLSSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDENYLK
DQIPCWLTYTTENSHTILRGNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFLEPEGRNTEEVYIGGLSTSMP
EDVQFDLVKSIPGLENAQMMRPGYAIEYDVVMPHQLRPTLETKLVSGLFTAGQTNGTSGYEEAAGQGLVAGINAALKVQG
KSEFILKRSEAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDEQWEHYQAKMAQFDREM
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVHYNEVIDFIGQAPEKIDRTVIELIETEITYEGYIKKAMD
QVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEGK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=40.9090909090909, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=39.3665158371041, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=38.4955752212389, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=48.1540930979133, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=625, Percent_Identity=39.68, Blast_Score=440, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=42.5806451612903, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=41.6666666666667, Blast_Score=444, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_LACLA (Q9CEJ4)

Other databases:

- EMBL:   AE005176
- PIR:   G86855
- RefSeq:   NP_268004.1
- ProteinModelPortal:   Q9CEJ4
- SMR:   Q9CEJ4
- GeneID:   1115512
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L97777
- NMPDR:   fig|272623.1.peg.1893
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   LLAC272623:L97777-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69378; Mature: 69378

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 182-182 BINDING 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQENYDVVVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVV
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCEEE
REIDALGGEMGRNIDKTYIQMKMLNTGKGPAVRALRAQADKDEYADSMKNTVSDQENLTL
EEHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
RQGMVEELILDEEKKKVIGIKTSTGTKYGAKAVIITTGTALRGEIIIGELKYSSGPNNSL
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCEECEEEEEEEEECCCCCCCH
SSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDENYLK
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
DQIPCWLTYTTENSHTILRGNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFL
HCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYIGGLSTSMPEDVQFDLVKSIPGLENAQMMRPGYAIEYDVVMPHQLRPTL
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHCCCCCEEEEEEECCCCCCCHH
ETKLVSGLFTAGQTNGTSGYEEAAGQGLVAGINAALKVQGKSEFILKRSEAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDEQWEHYQAKMAQFDREM
HHCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVHYNEVIDFIGQAPEKIDRT
HHHCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
VIELIETEITYEGYIKKAMDQVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LGQASRISGVNPADISILMVYLEGK
CCCHHHCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNFQENYDVVVIGGGHAGVEASLAAARMGSKTLLMTINLNMVAFMPCNPSIGGSAKGIVV
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCEEE
REIDALGGEMGRNIDKTYIQMKMLNTGKGPAVRALRAQADKDEYADSMKNTVSDQENLTL
EEHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
RQGMVEELILDEEKKKVIGIKTSTGTKYGAKAVIITTGTALRGEIIIGELKYSSGPNNSL
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCEECEEEEEEEEECCCCCCCH
SSIGLADNLREIGFEIGRFKTGTPPRVLASSIDYDKTEIQPGDEAPNHFSFMSSDENYLK
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
DQIPCWLTYTTENSHTILRGNLHRAPLFSGIVKGVGPRYCPSIEDKITRFADKPRHQLFL
HCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYIGGLSTSMPEDVQFDLVKSIPGLENAQMMRPGYAIEYDVVMPHQLRPTL
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHCCCCCEEEEEEECCCCCCCHH
ETKLVSGLFTAGQTNGTSGYEEAAGQGLVAGINAALKVQGKSEFILKRSEAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADRRLTEIGRQVGLVSDEQWEHYQAKMAQFDREM
HHCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KRLNSEKLKPLPDTQEKLGKLGFGPIKDALTGAEFLKRPEVHYNEVIDFIGQAPEKIDRT
HHHCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
VIELIETEITYEGYIKKAMDQVDKMHRLEAKRIPKNMDWDKLDSIATEARQKFKKINPET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LGQASRISGVNPADISILMVYLEGK
CCCHHHCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471