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The map label for this gene is pflA

Identifier: 15673795

GI number: 15673795

Start: 1880457

End: 1881251

Strand: Reverse

Name: pflA

Synonym: L63811

Alternate gene names: 15673795

Gene position: 1881251-1880457 (Counterclockwise)

Preceding gene: 15673796

Following gene: 15673794

Centisome position: 79.53

GC content: 35.47

Gene sequence:

>795_bases
ATGATGTCAGAGAATATAGATGAACTTAAAAAAGTTACTGGACTGATTCATTCAACTGAATCTTTTGGTTCTGTTGATGG
CCCTGGGGTCCGTTTTATTATTTTCATGCAAGGCTGTCGGATGCGTTGCAAATATTGTCACAACCCTGATACTTGGGCAT
TAAAGTCAGATAAAGCGACAGAGCGTACCGTAGAAGATGTCATGGATGAGGCACTTCGTTTTAGAGGTTTTTGGGGAGAG
AAAGGTGGAATTACCGTTTCTGGTGGTGAGGCGCTCCTTCAAATTGACTTTGTATTAGCTCTTTTCAAATATGCAAAATC
TCTCGGTATTCATACAACACTTGATACAGCGGCTCAACCATATTTGACTGATAAATATGTAACCGAAAAAATTGATGAGT
TACTAGATTATACCGACTTAGTATTATTAGACATTAAAGAAATAAATCCAGAACGACACAAAGAATTGACAGCTAATAAA
AACGATAATATTTTAGCTTTTGCACAGTATTTATCAGACCGTGGTAATGCAATGTGGGTTCGTCACGTTCTTGTTCCTGG
TGAAAGTGATTTTGATGAAGATTTAGTTCAATTAGGTGAATTTGTAAAAACTTTAAAAAATGTCTTGAAATTTGAAATTT
TACCCTACCATACAATGGGTGAATTTAAATGGCGTGAATTAGGTTGGAAATATCCGCTTGAAGGTGTGAAACCTCCAACA
AAAGATCGTGTTCATAATGCTAAAGAAATCATGAATACAGAATCTTATCAAGATTACTTAGAACGTATAAGATAA

Upstream 100 bases:

>100_bases
AGAGCCCTTGTCATTAACTTTTACTGACAGAATTTGATTTGATAAAATCAATGAATTGCGGTATGATAGGGGTGTGAAAT
AGTGTTTTGAATTGGAGAAG

Downstream 100 bases:

>100_bases
AAAATAAAAATGAATTTGAAGTTATTCAGATTCATTTTTTTGTTACAAAACGAACAATCAAATAAAATCAGTCCTAAATG
AAAATGAAAATGTGCTTAAT

Product: pyruvate-formate lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MMSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATERTVEDVMDEALRFRGFWGE
KGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTEKIDELLDYTDLVLLDIKEINPERHKELTANK
NDNILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPT
KDRVHNAKEIMNTESYQDYLERIR

Sequences:

>Translated_264_residues
MMSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATERTVEDVMDEALRFRGFWGE
KGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTEKIDELLDYTDLVLLDIKEINPERHKELTANK
NDNILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPT
KDRVHNAKEIMNTESYQDYLERIR
>Mature_264_residues
MMSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKATERTVEDVMDEALRFRGFWGE
KGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQPYLTDKYVTEKIDELLDYTDLVLLDIKEINPERHKELTANK
NDNILAFAQYLSDRGNAMWVRHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPT
KDRVHNAKEIMNTESYQDYLERIR

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=247, Percent_Identity=48.1781376518219, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1790839, Length=254, Percent_Identity=27.1653543307087, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=23.9852398523985, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30422; Mature: 30422

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKAT
CCCCCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEECCCCHH
ERTVEDVMDEALRFRGFWGEKGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
YLTDKYVTEKIDELLDYTDLVLLDIKEINPERHKELTANKNDNILAFAQYLSDRGNAMWV
CHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEE
RHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCC
KDRVHNAKEIMNTESYQDYLERIR
HHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MMSENIDELKKVTGLIHSTESFGSVDGPGVRFIIFMQGCRMRCKYCHNPDTWALKSDKAT
CCCCCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEECCCCHH
ERTVEDVMDEALRFRGFWGEKGGITVSGGEALLQIDFVLALFKYAKSLGIHTTLDTAAQP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
YLTDKYVTEKIDELLDYTDLVLLDIKEINPERHKELTANKNDNILAFAQYLSDRGNAMWV
CHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCCCCEEHHHHHHHCCCCEEEE
RHVLVPGESDFDEDLVQLGEFVKTLKNVLKFEILPYHTMGEFKWRELGWKYPLEGVKPPT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCC
KDRVHNAKEIMNTESYQDYLERIR
HHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]