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The map label for this gene is yrfD

Identifier: 15673681

GI number: 15673681

Start: 1755132

End: 1756532

Strand: Reverse

Name: yrfD

Synonym: L138484

Alternate gene names: 15673681

Gene position: 1756532-1755132 (Counterclockwise)

Preceding gene: 15673682

Following gene: 15673679

Centisome position: 74.25

GC content: 35.83

Gene sequence:

>1401_bases
ATGGAAAATGGAAAGGAAAAATTTAGTCTGTTTAGTGCTATTCTTTCTGTCATTTGTGTTGTTTTTGTTGCCGAAGCAGC
AGCACCGGTTGCTGCTATTGGTAACTCGCAGTTCTTTTGGTGGATTGTTCTCATCGTTGCTTTTCTCTTGCCTTATGGTC
TAATTACATCTGAACTTGGAACAACTTATACTGGTGAGGGTGGACTGTATGATTGGATTACAAAAGCCTTTGATCATCGT
TGGGGAGCGCGTGCTTCATGGTTTTATTGGGTAAACTTCCCATTATGGATGGCTTCACTTGCTGTACTTTGTCCTGATTT
ATTGCATACAATGTTTGGCTTTCAATTAAATACAGGTATTTCACTATTAATTGAATTAATTTTCATCTGGGTTATTGTTT
TGATTAGTTTATATCCAGTAAGTGATAGCGTTTGGATTTTAAATGGAGGCGCAGTAATTAAGGTATTCTTAGCATTAGCA
TTAGGAGGCTTAGGAGTTTATTCAGCTTTGACTAAGGGAGTAGCAAATCATTATACTTTAGCATCATTACTTCCAAGTTT
TGATTTGCATAGTCTATCATTTATCTCAGTAATCATTTTTAACTTACTGGGGTTTGAAGTCATTTGTACTTTTGCTGACA
GTATGGAAAATCCTAAGAAACAAATCCCACAATCAATCATCATTGGTGGGATTGTAATTGCAGCGATTTATATGTTTTCG
GCTTTTGGGATCGGTGTTGCTATTCCAACCAATAAATTATCGACAAGTTCGGGATTAGTAGATAGTTTCCAAGTGATGCT
AGGAACACCAACAGGTTGGTTCATTAGTTTAATTGCTTTCTTGTTTATGCTGACTTTAGTGGGTAATATGGTTTCTTGGT
CACAAGGGGTCAATAATATTGCTAGTTATGCTGCTGATAATGGTGATATGCCTAAATTCTTCTCAAAAAGACGTGCTTCT
AATGGAATTTCTTGGGGGGCAGCTTTGATGAATGGGATTGTAGCGACATTTATTGTTGTTATTGCTCCATTATTACCCAA
CCAAGATTTGTTCTGGTCATTCTTCTCATTAAATTTAGTACTATTTTTATTGAGTTATATTCCGGTCTTCCCTGCTTTTT
ATAAATTGCGCAAGATTGACCCTGAGACTCCACGTCCATTTAAAGTGAGTGGTAGTGACGGAATCTTGAAAGTATATATG
GCCTTACCAATGATTATTATTATCATATCATTAATTTTTACAGCCATACCCTTGCAATATGATAAGGCATCATTAACAGA
ACAATTACCGATTACAATTGGCGCAATTATTTTTATTGTGATTGGTGAATTAATTATTAAACGCAAATTGCAAGTCCAAG
TTAGCCAGCCAAGCTCATCTCATATGAACTCTTATAGTTGA

Upstream 100 bases:

>100_bases
GTCTGATTATGCTGAAAAAAATCCTTATGATTTGAAAGCACAAGCCAAAGCTAAGGCTGAGTTAGAGGCTTACTTAGCCA
AATAAGAAAGAGTATTATTT

Downstream 100 bases:

>100_bases
ACATTTTTCTTGGATAATGGTTCATCCAATACTCGATAGCGGCGACTTCTTCAGGGGTCGTCTTTGTTGTGCCTTTTGGC
AGATGCCTACGAATCATTCG

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MENGKEKFSLFSAILSVICVVFVAEAAAPVAAIGNSQFFWWIVLIVAFLLPYGLITSELGTTYTGEGGLYDWITKAFDHR
WGARASWFYWVNFPLWMASLAVLCPDLLHTMFGFQLNTGISLLIELIFIWVIVLISLYPVSDSVWILNGGAVIKVFLALA
LGGLGVYSALTKGVANHYTLASLLPSFDLHSLSFISVIIFNLLGFEVICTFADSMENPKKQIPQSIIIGGIVIAAIYMFS
AFGIGVAIPTNKLSTSSGLVDSFQVMLGTPTGWFISLIAFLFMLTLVGNMVSWSQGVNNIASYAADNGDMPKFFSKRRAS
NGISWGAALMNGIVATFIVVIAPLLPNQDLFWSFFSLNLVLFLLSYIPVFPAFYKLRKIDPETPRPFKVSGSDGILKVYM
ALPMIIIIISLIFTAIPLQYDKASLTEQLPITIGAIIFIVIGELIIKRKLQVQVSQPSSSHMNSYS

Sequences:

>Translated_466_residues
MENGKEKFSLFSAILSVICVVFVAEAAAPVAAIGNSQFFWWIVLIVAFLLPYGLITSELGTTYTGEGGLYDWITKAFDHR
WGARASWFYWVNFPLWMASLAVLCPDLLHTMFGFQLNTGISLLIELIFIWVIVLISLYPVSDSVWILNGGAVIKVFLALA
LGGLGVYSALTKGVANHYTLASLLPSFDLHSLSFISVIIFNLLGFEVICTFADSMENPKKQIPQSIIIGGIVIAAIYMFS
AFGIGVAIPTNKLSTSSGLVDSFQVMLGTPTGWFISLIAFLFMLTLVGNMVSWSQGVNNIASYAADNGDMPKFFSKRRAS
NGISWGAALMNGIVATFIVVIAPLLPNQDLFWSFFSLNLVLFLLSYIPVFPAFYKLRKIDPETPRPFKVSGSDGILKVYM
ALPMIIIIISLIFTAIPLQYDKASLTEQLPITIGAIIFIVIGELIIKRKLQVQVSQPSSSHMNSYS
>Mature_466_residues
MENGKEKFSLFSAILSVICVVFVAEAAAPVAAIGNSQFFWWIVLIVAFLLPYGLITSELGTTYTGEGGLYDWITKAFDHR
WGARASWFYWVNFPLWMASLAVLCPDLLHTMFGFQLNTGISLLIELIFIWVIVLISLYPVSDSVWILNGGAVIKVFLALA
LGGLGVYSALTKGVANHYTLASLLPSFDLHSLSFISVIIFNLLGFEVICTFADSMENPKKQIPQSIIIGGIVIAAIYMFS
AFGIGVAIPTNKLSTSSGLVDSFQVMLGTPTGWFISLIAFLFMLTLVGNMVSWSQGVNNIASYAADNGDMPKFFSKRRAS
NGISWGAALMNGIVATFIVVIAPLLPNQDLFWSFFSLNLVLFLLSYIPVFPAFYKLRKIDPETPRPFKVSGSDGILKVYM
ALPMIIIIISLIFTAIPLQYDKASLTEQLPITIGAIIFIVIGELIIKRKLQVQVSQPSSSHMNSYS

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=434, Percent_Identity=21.6589861751152, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51150; Mature: 51150

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENGKEKFSLFSAILSVICVVFVAEAAAPVAAIGNSQFFWWIVLIVAFLLPYGLITSELG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TTYTGEGGLYDWITKAFDHRWGARASWFYWVNFPLWMASLAVLCPDLLHTMFGFQLNTGI
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLLIELIFIWVIVLISLYPVSDSVWILNGGAVIKVFLALALGGLGVYSALTKGVANHYTL
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLPSFDLHSLSFISVIIFNLLGFEVICTFADSMENPKKQIPQSIIIGGIVIAAIYMFS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHH
AFGIGVAIPTNKLSTSSGLVDSFQVMLGTPTGWFISLIAFLFMLTLVGNMVSWSQGVNNI
HHCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYAADNGDMPKFFSKRRASNGISWGAALMNGIVATFIVVIAPLLPNQDLFWSFFSLNLV
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LFLLSYIPVFPAFYKLRKIDPETPRPFKVSGSDGILKVYMALPMIIIIISLIFTAIPLQY
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DKASLTEQLPITIGAIIFIVIGELIIKRKLQVQVSQPSSSHMNSYS
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure
MENGKEKFSLFSAILSVICVVFVAEAAAPVAAIGNSQFFWWIVLIVAFLLPYGLITSELG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TTYTGEGGLYDWITKAFDHRWGARASWFYWVNFPLWMASLAVLCPDLLHTMFGFQLNTGI
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLLIELIFIWVIVLISLYPVSDSVWILNGGAVIKVFLALALGGLGVYSALTKGVANHYTL
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLPSFDLHSLSFISVIIFNLLGFEVICTFADSMENPKKQIPQSIIIGGIVIAAIYMFS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHH
AFGIGVAIPTNKLSTSSGLVDSFQVMLGTPTGWFISLIAFLFMLTLVGNMVSWSQGVNNI
HHCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYAADNGDMPKFFSKRRASNGISWGAALMNGIVATFIVVIAPLLPNQDLFWSFFSLNLV
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LFLLSYIPVFPAFYKLRKIDPETPRPFKVSGSDGILKVYMALPMIIIIISLIFTAIPLQY
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DKASLTEQLPITIGAIIFIVIGELIIKRKLQVQVSQPSSSHMNSYS
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]