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The map label for this gene is dexC

Identifier: 15673664

GI number: 15673664

Start: 1736928

End: 1738682

Strand: Reverse

Name: dexC

Synonym: L128694

Alternate gene names: 15673664

Gene position: 1738682-1736928 (Counterclockwise)

Preceding gene: 15673671

Following gene: 15673663

Centisome position: 73.5

GC content: 36.18

Gene sequence:

>1755_bases
ATGAACAAAGCTGCAATTTATCATCGTCCTGAGTCGGAATTTGCTTATCTTTATGCAAAAAATCTGATGCATGTTCGTCT
GCAAACACAAAAATCTGATGTGGAAAAAGTCGAATTATTTTATGGCGATCCTTATTCGTGGGAAGGGCTTGATAACCCTG
AAACACAGTATTGGGCTAGTCAAAAAGTAGAGATGAAAAAGTATCTTTCTACTAATATTAGCGATTATTTTGAGGCCGAA
ATTACTGTTGCAACGAAACGAGTAGATTACTTATTTGTCATTACAGGTTTGGATGGAGAAAAAGTAGTTTACACAGACCA
AGGAATGTTTGATTATGATGAGAGCTTGGTGACTAAAAAATATGGGGCTTTCCGTTTGCCTTATTTCCATGAAAGTGATC
GTTTTAAAGCACCTGAATGGGTCAAAGAAACGGTTTGGTATCAAATTTTCCCTGAACGTTTTGCCAATGGGGATACGAGC
AATGATCCTGAGGGAACAAAAGCTTGGAATCCACAAGATGTTCCTGACCGTCAAGATTTTTATGGGGGAGATTTGCAAGG
GGTGATTGATCATTTGGAACATTTGACTGAACTCGGTGTGAATGGCATTTACTTTACTCCTATTTTCCAAGCTTTTTCTA
ATCATAAATATGATACAGAAGATTATATGGAAATTGATAAGCAATTTGGGGATAAGGAATTGTTTAAAACTTTGGTGAAA
GAAGCACATGCGCGTGGTATTAAAGTCATGTTAGATGCGGTCTTTAATCACATCGGAGATACGTCTGCTCAATGGCAAGA
TGTATTGAAAAATCAAGAAAAATCAAAATTTGCGGATTGGTTCCATGTTAATAGTTGGCCAGCAACTTATACTCCGACTG
ATGATTTTGAAAATGCTGAGAATGCAACTTATGATACTTTTGCCTTTACTCCACACATGCCAAAACTCAATACGGCTAAC
CCAGAGGTTGAAGCTTATCTTTTGAAAGTAGCTGATTATTGGATTTCTGAGTTTGATATTGATGCTTGGCGTTTGGATGT
GGCGGATGAAGTGGATCATACTTTCTGGAAAAAATTCCGTACAACTTGTGACGCGGCAAAAGATGATTTTTATATTTTAG
GTGAGGTTTGGCATTCTGCACAACCTTGGTTAGTTGGTGACGAATTCTCTGCCGTTATGAACTATGCTTATACTGATGCG
ATTAAAGATGGTTTGATTACGAAGAAGATTTCTTTGGAACAAATGGTTTCTAATATTAATACGCAACTGACATTATACCG
TAAGCAAGTGAATCAAATGATGTTTAATGTTTTAGATTCTCATGATACACCCAGAATTTTGACTGTTGCAAAAAATAATA
AAAATTTGATGAAATTGGTTGAAACGTTCACCTTCATGCAACCAGGTGTGCCTTGTATTTATTATGGGGATGAGTATGCG
GTGACAGGTGGAATGGACCCTGAGTGTCGTAAGGTGATGCCTTGGGAAGAAAAAGATCAAGATTTAGAGATGTTTGATTT
CTTTAAAAATCTGATTGCTCTACGCAAACAATATCAAGGAATTTTAAGTGATTCAGAAGTAGAATGGAAAATCGTAGATT
CAGAAAATGGCATTGTGAAATTTGAACGTGGATCTTTGAAAGCTATTTTCAATATTGGCGAAAAACCGGCTTATTTTTCA
GCTGATGAAGTGGTTTTCTCTAATTTGGTTGACCAAAATAATATTTTACAATATGGTTTTGTAATCTATAAATAG

Upstream 100 bases:

>100_bases
CTATAGAAAAGCGACGGAAAAGGTGCTTTTCGCTGTCCTTGAGATTAATTGAAAATAGTTTAAGACAGTACAGCCATCAC
AAAAATTTGGAGGTTCTATT

Downstream 100 bases:

>100_bases
AAAACGGATTCTGTCAGTTCACTGCGACCGAGGGAATCATTGAATCGGTAAATAAAGAAACTATCGGCTTTGCTGATAGT
TTTGTCAGTAAAAATTGCTG

Product: neopullulanase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGSLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK

Sequences:

>Translated_584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGSLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK
>Mature_584_residues
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVEMKKYLSTNISDYFEAE
ITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTS
NDPEGTKAWNPQDVPDRQDFYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYDTFAFTPHMPKLNTAN
PEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDA
IKDGLITKKISLEQMVSNINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVKFERGSLKAIFNIGEKPAYFS
ADEVVFSNLVDQNNILQYGFVIYK

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=228, Percent_Identity=24.5614035087719, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786604, Length=443, Percent_Identity=31.3769751693002, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1790687, Length=417, Percent_Identity=25.6594724220624, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1789995, Length=466, Percent_Identity=24.2489270386266, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322245, Length=228, Percent_Identity=25.4385964912281, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321726, Length=332, Percent_Identity=23.4939759036145, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=334, Percent_Identity=22.7544910179641, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=289, Percent_Identity=24.2214532871972, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=289, Percent_Identity=24.2214532871972, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221330053, Length=408, Percent_Identity=24.0196078431373, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586587, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586599, Length=418, Percent_Identity=23.9234449760766, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583747, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583749, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583745, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586593, Length=436, Percent_Identity=22.2477064220184, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24586591, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24586589, Length=283, Percent_Identity=25.0883392226148, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549022, Length=225, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 68036; Mature: 68036

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWAS
CCCCEEEECCCCCEEHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
QKVEMKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKK
HHHHHHHHHHCCCHHHHEEEEEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHC
YGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDF
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
YGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHH
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAE
HHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCCCCC
NATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFR
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
TTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNIN
HHHCCCCCCEEEEEEHHCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
TQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEE
VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVK
EECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
FERGSLKAIFNIGEKPAYFSADEVVFSNLVDQNNILQYGFVIYK
EECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECEEEEC
>Mature Secondary Structure
MNKAAIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWAS
CCCCEEEECCCCCEEHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
QKVEMKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKK
HHHHHHHHHHCCCHHHHEEEEEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHC
YGAFRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDF
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
YGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVK
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHH
EAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAE
HHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCCCCC
NATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFR
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
TTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNIN
HHHCCCCCCEEEEEEHHCCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
TQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCEEE
VTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSENGIVK
EECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
FERGSLKAIFNIGEKPAYFSADEVVFSNLVDQNNILQYGFVIYK
EECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]