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The map label for this gene is rliA

Identifier: 15673658

GI number: 15673658

Start: 1727815

End: 1728789

Strand: Reverse

Name: rliA

Synonym: L0144

Alternate gene names: 15673658

Gene position: 1728789-1727815 (Counterclockwise)

Preceding gene: 15673659

Following gene: 15673657

Centisome position: 73.08

GC content: 35.69

Gene sequence:

>975_bases
ATGGCAGTTACAATTAAAGATGTTGCAAAAAAAGCTGGTGTTAATGCCTCAACGGTCAGTCGGGTCATTAAAGATAGTTC
AGAGATTTCAGATAAAACAAAAGTAAAAGTTCGCAAAGCCATGCATGAATTAGGTTATCGAAGGAATGCAGCGGCTCAGA
TTCTAGCTTCGGGAAAAACAAATACAATTGGTGTTGTTTTCCCGCCAGTGGCTGACAAAGCGAGCCAACCTTTCTTTATG
AAAATATTGACCTCAATCAATGAAACAGCTCGTGATTACGATGTTTCAATCGCTATTGCGACGGGGCATTCAGCCAAAGA
GTTGAAAAAACAGATTGAACTTCAATATTCAGAAAAGCGAGTTGACGGATTTATCGTGCTTTACGCTGGAAAAAATGATG
AAGTTCGCGATTATTTATTAGAAAATAAGGTTCCTTTTGTTTTAGTTGGAACTCCGGCTGAAGGAAAAAATGAAATTACC
CATGTGGATAATGATAATATGCTGCTTGGACGCGAGGCTGTTCGTCATTTAGCACAATTAAAACATCAAAATATTTCATT
TGTGACCGATACGAAAGAAACAGAAGTTTTTGAAGAACGTTATCAAGGTTTTAAAGATGAATCCGAGAAACTTGGGCTCA
ATCATGACCTCTTGTTTATGGATGCTAATTTTTCCTTGCGAAATGAAACAGCTCTTGTAGTTATGGATGATGTCTTGTCT
TTAAAAGTTGTGGAACGTCTAAGAAGTCAAGGTCTAAATGTTCCAGAAGATGTGAGTTTAATTACTTACAACAACTCTAT
CTTTGGAGCAATGATTCATCCTTATTTAACAACTTTTGACATTCATATTGAGCAATTAGGGGCAAGTGCAATTAAGAAAA
TCTTGGACTTACGAGATAAGAAAGAAAATTTGCCAGAAAAATCAATTATCCCTTTTGAATTAATTATTAGAGAATCAACA
AAAGCTAGAATTTAA

Upstream 100 bases:

>100_bases
TTAATCGAAAATTTGGCAAAATAAAATTTCAGAGTGTCAAATAAAAGAAAGAGAAAGGAGCGGTGAGTTGAGCTGGGTTT
AGCTAGTCTCAAGTGAAAAT

Downstream 100 bases:

>100_bases
GTTCTAGCTTTTTTAGTTTTGTTTAAGATAAGGTTAAGCCTAGTACAAGACAAAAATATGATAATAGTGTAAAATAATAG
AAAGGGTTGGGCGCAGTTTT

Product: transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAVTIKDVAKKAGVNASTVSRVIKDSSEISDKTKVKVRKAMHELGYRRNAAAQILASGKTNTIGVVFPPVADKASQPFFM
KILTSINETARDYDVSIAIATGHSAKELKKQIELQYSEKRVDGFIVLYAGKNDEVRDYLLENKVPFVLVGTPAEGKNEIT
HVDNDNMLLGREAVRHLAQLKHQNISFVTDTKETEVFEERYQGFKDESEKLGLNHDLLFMDANFSLRNETALVVMDDVLS
LKVVERLRSQGLNVPEDVSLITYNNSIFGAMIHPYLTTFDIHIEQLGASAIKKILDLRDKKENLPEKSIIPFELIIREST
KARI

Sequences:

>Translated_324_residues
MAVTIKDVAKKAGVNASTVSRVIKDSSEISDKTKVKVRKAMHELGYRRNAAAQILASGKTNTIGVVFPPVADKASQPFFM
KILTSINETARDYDVSIAIATGHSAKELKKQIELQYSEKRVDGFIVLYAGKNDEVRDYLLENKVPFVLVGTPAEGKNEIT
HVDNDNMLLGREAVRHLAQLKHQNISFVTDTKETEVFEERYQGFKDESEKLGLNHDLLFMDANFSLRNETALVVMDDVLS
LKVVERLRSQGLNVPEDVSLITYNNSIFGAMIHPYLTTFDIHIEQLGASAIKKILDLRDKKENLPEKSIIPFELIIREST
KARI
>Mature_323_residues
AVTIKDVAKKAGVNASTVSRVIKDSSEISDKTKVKVRKAMHELGYRRNAAAQILASGKTNTIGVVFPPVADKASQPFFMK
ILTSINETARDYDVSIAIATGHSAKELKKQIELQYSEKRVDGFIVLYAGKNDEVRDYLLENKVPFVLVGTPAEGKNEITH
VDNDNMLLGREAVRHLAQLKHQNISFVTDTKETEVFEERYQGFKDESEKLGLNHDLLFMDANFSLRNETALVVMDDVLSL
KVVERLRSQGLNVPEDVSLITYNNSIFGAMIHPYLTTFDIHIEQLGASAIKKILDLRDKKENLPEKSIIPFELIIRESTK
ARI

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=27.5964391691395, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789202, Length=319, Percent_Identity=27.8996865203762, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=26.3843648208469, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=24.9221183800623, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789068, Length=343, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=25.8258258258258, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=27.0833333333333, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788474, Length=342, Percent_Identity=26.6081871345029, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787906, Length=214, Percent_Identity=29.4392523364486, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790715, Length=301, Percent_Identity=21.2624584717608, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36361; Mature: 36230

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTIKDVAKKAGVNASTVSRVIKDSSEISDKTKVKVRKAMHELGYRRNAAAQILASGKT
CEEEHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
NTIGVVFPPVADKASQPFFMKILTSINETARDYDVSIAIATGHSAKELKKQIELQYSEKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCC
VDGFIVLYAGKNDEVRDYLLENKVPFVLVGTPAEGKNEITHVDNDNMLLGREAVRHLAQL
CCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEECHHHHHHHHHH
KHQNISFVTDTKETEVFEERYQGFKDESEKLGLNHDLLFMDANFSLRNETALVVMDDVLS
HCCCCEEEECCHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEEEHHHHH
LKVVERLRSQGLNVPEDVSLITYNNSIFGAMIHPYLTTFDIHIEQLGASAIKKILDLRDK
HHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KENLPEKSIIPFELIIRESTKARI
HHCCCCCCCCCHHEEEECCCCCCC
>Mature Secondary Structure 
AVTIKDVAKKAGVNASTVSRVIKDSSEISDKTKVKVRKAMHELGYRRNAAAQILASGKT
EEEHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
NTIGVVFPPVADKASQPFFMKILTSINETARDYDVSIAIATGHSAKELKKQIELQYSEKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCC
VDGFIVLYAGKNDEVRDYLLENKVPFVLVGTPAEGKNEITHVDNDNMLLGREAVRHLAQL
CCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEECHHHHHHHHHH
KHQNISFVTDTKETEVFEERYQGFKDESEKLGLNHDLLFMDANFSLRNETALVVMDDVLS
HCCCCEEEECCHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEEEHHHHH
LKVVERLRSQGLNVPEDVSLITYNNSIFGAMIHPYLTTFDIHIEQLGASAIKKILDLRDK
HHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KENLPEKSIIPFELIIRESTKARI
HHCCCCCCCCCHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]