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The map label for this gene is purB

Identifier: 15673623

GI number: 15673623

Start: 1688439

End: 1689734

Strand: Reverse

Name: purB

Synonym: L88187

Alternate gene names: 15673623

Gene position: 1689734-1688439 (Counterclockwise)

Preceding gene: 15673624

Following gene: 15673622

Centisome position: 71.43

GC content: 37.96

Gene sequence:

>1296_bases
GTGGTTATTAGCCGTTATTCACGTCCAGAAATGGCAGAGATTTGGTCAGAAGAAAACAAGTATAAAGCATGGCTTGAAGT
CGAAATTCTTGCAGATGAAGCATGGGCAGAGCTTGGGGAAATCCCAGCAGCAGATGTGAAAAAAATTCGCGAAAATGCAA
CTTTTAATGTTGAACGTATTCTTGAAATTGAAAAAGAAACACGTCATGATGTGGTAGCTTTCACACGTTCTGTCAGTGAA
TCATTGGGTGAAGAACGCAAATGGGTTCATTATGGTCTGACATCAACAGATGTCGTTGATACAGCTTATGGTTATCTTTA
TAAACAAGCTAATGATATTTTGCGCAATGATCTAAAAAACTTTGCAGAAATTGTTAAAAATAAAGCTTTAGAGCATAAAT
ACACAGTAATGATGGGTCGAACTCACGGCGTTCACGCTGAACCAACAACTTTTGGCTTGAAATTAGCGACTTGGTACAGC
GAAATGAAACGAAATATTGAACGTTTTGAACGTGCTGCAAAAGCTGTTGAAGCTGGGAAAATTTCTGGTGCTGTTGGAAC
TTTTGCTAATATTCCTCCATTTGTAGAAGCCTATGTTTGTGAAAAATTAGGGACTCGTCCGCAAGAGATTTCAACACAAG
TCTTACCACGTGATTTACATGCAGAATACTTCCAAACGTTGGCGCTCATTGCGACATCAATTGAACGCATGGCCACAGAA
ATCCGTGGATTACAAAAATCTGAACAACGTGAAGTTGAAGAATTCTTTGCTAAAGGTCAAAAAGGCTCGTCAGCAATGCC
ACACAAACGTAACCCAATTGGTTCTGAAAATATGACAGGTTTAGCCCGCGTCATTCGTGGACATGCAATTACAGCTCTTG
AAGATGTTAGTCTTTGGCATGAACGTGACATTTCACACAGTTCTGCTGAACGTATCATTGCACCTGATACAACAGAATTA
TTGGATTATATGTTGAATCGTTTTGGGAATATCGTTAAAAATTTGACTGTTTTCCCAGAAAATATGAAGCGAAATATGGA
TGCGACTTTTGGCTTGATTTACAGCCAACATGTCATGTTAATGTTGATTGAAAAGGGAATGACGCGTGAAGCAGCTTACG
ATTTAGTTCAACCTTTGACTGCAAAATCTTGGGATGAACAAGTGATGTTTAGACCATTGCTTGAAGAAAATGAACAAATT
CGTCAGTATTTGAATGATGAAGATATTGATAAAGCTTTTGATTACAATTATCATTTAACAAGAGTCGATGAAATTTTTGA
GCGTGTCTTTGATTAA

Upstream 100 bases:

>100_bases
ATGTTATAATAGATAATGAAAAAGGACGCTTTGTTCATTAGAAATTAAAAATAGGATTAATTTACTGACAGCTATTCTGT
CAGTAAAAAAGGAGAAAAAA

Downstream 100 bases:

>100_bases
TAAGAAAGCCTACTAAAAAGTAGGTTTTTTTGTTATTAAAACGAAATATAAAAAGGATTGACAAATTTAATGAAAACGGA
TACAATTATAAATGTAAATA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPAADVKKIRENATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRNDLKNFAEIVKNKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFERAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RQYLNDEDIDKAFDYNYHLTRVDEIFERVFD

Sequences:

>Translated_431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPAADVKKIRENATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRNDLKNFAEIVKNKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFERAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RQYLNDEDIDKAFDYNYHLTRVDEIFERVFD
>Mature_431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPAADVKKIRENATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRNDLKNFAEIVKNKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFERAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RQYLNDEDIDKAFDYNYHLTRVDEIFERVFD

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=397, Percent_Identity=27.9596977329975, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=27.7078085642317, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=30.3405572755418, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=397, Percent_Identity=28.463476070529, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=336, Percent_Identity=29.4642857142857, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=397, Percent_Identity=27.9596977329975, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24647570, Length=420, Percent_Identity=24.047619047619, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49688; Mature: 49688

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPAADVKKIRENATFNVERI
CCCCCCCCCHHHHHHCCCCCEEEEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
LEIEKETRHDVVAFTRSVSESLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRNDLKN
HHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAEIVKNKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFERAAKAVEAGK
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
CCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ERDISHSSAERIIAPDTTELLDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVML
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHH
MLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQIRQYLNDEDIDKAFDYNYHLT
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHH
RVDEIFERVFD
HHHHHHHHHCC
>Mature Secondary Structure
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPAADVKKIRENATFNVERI
CCCCCCCCCHHHHHHCCCCCEEEEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
LEIEKETRHDVVAFTRSVSESLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRNDLKN
HHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAEIVKNKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFERAAKAVEAGK
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
CCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ERDISHSSAERIIAPDTTELLDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVML
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHH
MLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQIRQYLNDEDIDKAFDYNYHLT
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHH
RVDEIFERVFD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]