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The map label for this gene is pyrB

Identifier: 15673584

GI number: 15673584

Start: 1645253

End: 1646185

Strand: Reverse

Name: pyrB

Synonym: L45002

Alternate gene names: 15673584

Gene position: 1646185-1645253 (Counterclockwise)

Preceding gene: 15673585

Following gene: 15673583

Centisome position: 69.59

GC content: 37.94

Gene sequence:

>933_bases
ATGTCAGTAAAAAATGGATTAGTTCAACTCGAAAACCTCACAAGTATGGAAAAATTGAGTGTTGATGAAGTCATGGGACT
TATCAAAAGAGCCTCAGCTTTCAAAGCAGGAACAGCAGAATTTGATTTAGAAAAACAAACTTTTGCTTCAAATCTTTTCT
TTGAAAATTCTACAAGAACGCACCATAGTTTCCATATTGCTGAGCGTAAATTAGGCTTAGATGTTCTTGAATTTGATGCG
CAAGCAAGCTCAATTAGTAAAGGAGAAACTCTTTACGATACCGTCTTAACACTTGATGCACTTGGCGTTGATATCTGTGT
TATACGTTCAGGAGTAGAACATTACTATGAAGAATTAGTGAATTCAGATAATATTCATTGTGCTATTGTTAACGGTGGAG
ATGGTTCAGGGCAACATCCAAGTCAATGTTTACTTGACTTGATGACCATTTATGAAGAATTTGGCAAATTTGAAGGTTTA
AAAATCGCAATTTCCGGAGATTTAACCCATTCACGTGTCGCCAAATCTAATATGATGATGCTCCAAAAATTAGGAGCTAG
ACTTTACTTTACAGGCCCAGCAGCATGGTATAGTGAAGAATTTGATGATTACGGTCATTATGCCAACCTAGACCGAATTT
TGCCAGAACTTGACGTTCATATGCTCTTACGCGTTCAACACGAACGTCATGATAGTGGCGAAAGCTTTTCTAAAGAAGGC
TACCATAATCATTTTGGACTGACAGAAGAACGAGCAAAAATGTTGAAACCAACAGCCATTATCATGCACCCAGCTCCAGT
CAATCGGGGTGTTGAAATTGCTGACAGCCTTGTTGAAAGCCCACAAAGTCGGATTGTTCAACAAATGAGCAATGGTGTTT
ATACGCGAATGGCAATTCTTGAAGCAATTCTTGCTGGAAAAAAAGCTAAATAA

Upstream 100 bases:

>100_bases
AGTAGATAAAAATTTTGTCAGTTTACTGACAAAACAATCAAATATATTGTCAATAAAACTGTTGTGACAGAGAGCACACA
GTCAGGGTAAAGGAGACCTC

Downstream 100 bases:

>100_bases
TTCAAAGCGCTGACAACAAGAAAAAATAATTGCTAAAGCAAAATTCAAAGGAAATATTTATTATGAGCAAAAGACTTTTA
ATTTTAGAAGACGGAACCAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSVKNGLVQLENLTSMEKLSVDEVMGLIKRASAFKAGTAEFDLEKQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDA
QASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGL
KIAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
YHNHFGLTEERAKMLKPTAIIMHPAPVNRGVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK

Sequences:

>Translated_310_residues
MSVKNGLVQLENLTSMEKLSVDEVMGLIKRASAFKAGTAEFDLEKQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDA
QASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGL
KIAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
YHNHFGLTEERAKMLKPTAIIMHPAPVNRGVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK
>Mature_309_residues
SVKNGLVQLENLTSMEKLSVDEVMGLIKRASAFKAGTAEFDLEKQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDAQ
ASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLK
IAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEGY
HNHFGLTEERAKMLKPTAIIMHPAPVNRGVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=30.8441558441558, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=32.6923076923077, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI193204318, Length=282, Percent_Identity=35.4609929078014, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=292, Percent_Identity=31.8493150684932, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24642586, Length=267, Percent_Identity=32.2097378277154, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_LACLA (Q9CF79)

Other databases:

- EMBL:   AE005176
- PIR:   B86825
- RefSeq:   NP_267758.1
- ProteinModelPortal:   Q9CF79
- SMR:   Q9CF79
- GeneID:   1115262
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L45002
- NMPDR:   fig|272623.1.peg.1644
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   LLAC272623:L45002-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34559; Mature: 34428

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKNGLVQLENLTSMEKLSVDEVMGLIKRASAFKAGTAEFDLEKQTFASNLFFENSTRT
CCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCC
HHSFHIAERKLGLDVLEFDAQASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELV
CHHHHHHHHHHCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
NSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLKIAISGDLTHSRVAKSNMMM
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
HHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YHNHFGLTEERAKMLKPTAIIMHPAPVNRGVEIADSLVESPQSRIVQQMSNGVYTRMAIL
CCCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
EAILAGKKAK
HHHHCCCCCC
>Mature Secondary Structure 
SVKNGLVQLENLTSMEKLSVDEVMGLIKRASAFKAGTAEFDLEKQTFASNLFFENSTRT
CCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCC
HHSFHIAERKLGLDVLEFDAQASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELV
CHHHHHHHHHHCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
NSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLKIAISGDLTHSRVAKSNMMM
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
HHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YHNHFGLTEERAKMLKPTAIIMHPAPVNRGVEIADSLVESPQSRIVQQMSNGVYTRMAIL
CCCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
EAILAGKKAK
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471