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The map label for this gene is carA

Identifier: 15673583

GI number: 15673583

Start: 1644117

End: 1645190

Strand: Reverse

Name: carA

Synonym: L43866

Alternate gene names: 15673583

Gene position: 1645190-1644117 (Counterclockwise)

Preceding gene: 15673584

Following gene: 15673582

Centisome position: 69.55

GC content: 39.66

Gene sequence:

>1074_bases
ATGAGCAAAAGACTTTTAATTTTAGAAGACGGAACCATTTTTGAGGGAGAATCCCTTGGTGCAAACTTGGATGTTACAGG
TGAACTTGTCTTTAATACAGGAATGACAGGCTATCAAGAATCAATCACTGACCAATCATACAACGGACAAATTTTGACTT
TCACTTATCCAATTGTTGGAAATTATGGCGTCAATCGAGATGATTATGAATCAATTCATCCAACCTGTAAAGCCGTAGTT
GTTCATGAAGCAGCGTGCCGTCCTTCGAACTGGCGGATGCAAATGTCTTTTGATGAATTTTTAAAAGCAAAAAATATTCC
TGGAATTACAGGTGTGGATACTCGAGCGATTACTAAAATTGTTCGAGAACACGGCACTATGAAGGCCTCACTTGTACAAG
CAAGAGATGAAGTAGAGCATCAAATGAGCCAACTTCAAGCGACAGTATTACCAACAAATCAAGTTGAAACAAGTAGTACA
ACGACCGCTTATCCATCACCAAATACAGGCAGAAAAGTTGTCGTTGTTGACTTTGGCTTGAAACATAGTATCCTACGGGA
ACTTTCAAAACGGGAATGTAATCTCACCGTTGTCCCATACAACACCAGTGCTCAAGAAATTTTGGAAATGGAACCTGATG
GTGTCATGTTGACTAATGGCCCTGGTGACCCAACAGATGTACCAGAAGCAATTGAAATGATTAAAGAAATTCAAGGTAAA
ATTCCAATTTTCGGTATTTGCTTAGGTCATCAATTATTTAGTCTTGCAAATGGAGCAAAAACCTACAAAATGAAATTTGG
ACACCGTGGATTTAACCATGCCGTTCGTGAAATTGCAACAGGACGAATTGACTTCACCTCACAAAATCATGGATATGCCG
TTTCATCAGAAAACTTACCTGAAGACCTAATGATTACCCATGTTGAAATCAATGACGACTCAGTTGAAGGAGTGCGTCAC
AAACACTTCCCAGCTTTTTCAGTCCAATTTCACCCAGACGCAGCTCCTGGGCCTCACGATGCCAGCTATCTTTTTGATGA
TTTCATGGATCTCATGGATAACTTCAAAAAATAG

Upstream 100 bases:

>100_bases
TTCTTGAAGCAATTCTTGCTGGAAAAAAAGCTAAATAATTCAAAGCGCTGACAACAAGAAAAAATAATTGCTAAAGCAAA
ATTCAAAGGAAATATTTATT

Downstream 100 bases:

>100_bases
GATATTGATATAAATGTCTTTGAGTGTGTGACAGTATATTGCTCGTTAGATAATAAAAAAACTCAGAACTACTGAGTTTT
TTTATTATCTATTTTTGTAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKRLLILEDGTIFEGESLGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVV
VHEAACRPSNWRMQMSFDEFLKAKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVEHQMSQLQATVLPTNQVETSST
TTAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAQEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEIQGK
IPIFGICLGHQLFSLANGAKTYKMKFGHRGFNHAVREIATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDDSVEGVRH
KHFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK

Sequences:

>Translated_357_residues
MSKRLLILEDGTIFEGESLGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVV
VHEAACRPSNWRMQMSFDEFLKAKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVEHQMSQLQATVLPTNQVETSST
TTAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAQEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEIQGK
IPIFGICLGHQLFSLANGAKTYKMKFGHRGFNHAVREIATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDDSVEGVRH
KHFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
>Mature_356_residues
SKRLLILEDGTIFEGESLGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVVV
HEAACRPSNWRMQMSFDEFLKAKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVEHQMSQLQATVLPTNQVETSSTT
TAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAQEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEIQGKI
PIFGICLGHQLFSLANGAKTYKMKFGHRGFNHAVREIATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDDSVEGVRHK
HFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=359, Percent_Identity=40.3899721448468, Blast_Score=274, Evalue=9e-74,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=37.874659400545, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=37.874659400545, Blast_Score=228, Evalue=8e-60,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=42.4, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI193204318, Length=362, Percent_Identity=41.7127071823204, Blast_Score=260, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324878, Length=371, Percent_Identity=39.622641509434, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=37.6262626262626, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322638, Length=142, Percent_Identity=31.6901408450704, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45555749, Length=368, Percent_Identity=39.4021739130435, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24642586, Length=368, Percent_Identity=39.4021739130435, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_LACLA (Q9CF80)

Other databases:

- EMBL:   AE005176
- PIR:   A86825
- RefSeq:   NP_267757.1
- ProteinModelPortal:   Q9CF80
- GeneID:   1115261
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L43866
- NMPDR:   fig|272623.1.peg.1643
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   LLAC272623:L43866-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39658; Mature: 39527

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 247-247 ACT_SITE 331-331 ACT_SITE 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLLILEDGTIFEGESLGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVG
CCCEEEEEECCCEECCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NYGVNRDDYESIHPTCKAVVVHEAACRPSNWRMQMSFDEFLKAKNIPGITGVDTRAITKI
CCCCCCCCHHHHCCHHEEEEEECCCCCCCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHH
VREHGTMKASLVQARDEVEHQMSQLQATVLPTNQVETSSTTTAYPSPNTGRKVVVVDFGL
HHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCC
KHSILRELSKRECNLTVVPYNTSAQEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEIQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
IPIFGICLGHQLFSLANGAKTYKMKFGHRGFNHAVREIATGRIDFTSQNHGYAVSSENLP
CCEEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCEEECCCCCC
EDLMITHVEINDDSVEGVRHKHFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
CCCEEEEEEECCCCCCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKRLLILEDGTIFEGESLGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVG
CCEEEEEECCCEECCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NYGVNRDDYESIHPTCKAVVVHEAACRPSNWRMQMSFDEFLKAKNIPGITGVDTRAITKI
CCCCCCCCHHHHCCHHEEEEEECCCCCCCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHH
VREHGTMKASLVQARDEVEHQMSQLQATVLPTNQVETSSTTTAYPSPNTGRKVVVVDFGL
HHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCC
KHSILRELSKRECNLTVVPYNTSAQEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEIQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
IPIFGICLGHQLFSLANGAKTYKMKFGHRGFNHAVREIATGRIDFTSQNHGYAVSSENLP
CCEEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCEEECCCCCC
EDLMITHVEINDDSVEGVRHKHFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
CCCEEEEEEECCCCCCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471