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The map label for this gene is purF

Identifier: 15673510

GI number: 15673510

Start: 1571601

End: 1573100

Strand: Reverse

Name: purF

Synonym: L171350

Alternate gene names: 15673510

Gene position: 1573100-1571601 (Counterclockwise)

Preceding gene: 15673511

Following gene: 15673509

Centisome position: 66.5

GC content: 44.53

Gene sequence:

>1500_bases
ATGTTTGGCAAAGAACAGACGCTTGATTTAAGAGTGCTGGGGAAGAATTCGTCCAATGAAAATAGTTGGTGGCAACAACA
TCCAGATGACGAACGATCTCATTTAAATGAAGAATGTGGACTTTTCGGTGTTTTGGGACATCCTGATGCTGCTCGCCTGA
CCTATTTTGGACTTCATGCTCTCCAACATCGCGGGCAAGAAGGCGCAGGGATTTTGGTCAATAATAATGGCAAACTCAAT
CGTCATCGTGGTCTGGGTCTGGTCACAGAAGTTTTCCGTGACGAAAAAGATTTGGAAGAGCTGACAGGCTCGTCAGCCAT
TGGACACGTCCGTTATGCCACGGCTGGTTCAGCAAATATCAATAATATTCAGCCTTTCCAGTTTGAATTTCATGACGGAG
CACTTGGACTTGCCCACAATGGAAATTTAACCAACGCCCAATCATTGCGCTGTGAACTTGAAAAATCTGGGGCAATCTTT
TCTAGTAATTCAGACACGGAAATTCTCATGCACTTGATTCGCCGTAGTCATCATCCAGAATTTATGGGCAGAGTCAAAGA
AGCGCTCAATACGGTCAAAGGTGGCTTTGCCTATCTCATTATGACTGAAAACTCAATTGTCGCAGCCCTTGATCCTAATG
GATTTCGACCACTCTCAATCGGAAAAATGAGCAATGGTGCGCTCGTTGTGGCCTCAGAAACCTGTGCTTTTGATGTCGTT
GGTGCCACTTGGATTCAAGATGTTCAACCCGGTGAAATCATTGAAATTAATGACGACGGCATTCATGTGGACCAATTTAC
TGACAGCACCAACATGACCATTTGTTCAATGGAATACATCTATTTTGCCCGTCCTGACAGTAATATTGCTGGAGTTAATG
TTCATACTGCCAGAAAACGTTCAGGAAAAATTTTAGCCCAAGAAGCCCAAATTGATGCTGATATTGTCATCGGTGTGCCT
AATTCATCACTCAGTGCCGCTTCAGGCTACGCTGAAGAATCTGGCTTGCCTTACGAAATGGGCCTGATTAAAAATCAGTA
TGTGGCAAGAACCTTCATTCAACCGACTCAAGAACTTCGTGAACAAGGCGTTCGCATGAAATTGAGTGCTGTTCGAGGTG
TTGTAGAAGGCAAACGTGTCATTATGGTGGATGATAGTATTGTGCGTGGGACAACCAGCCGTCGCATTGTCAAACTCCTC
AAAGACGCAGGCGCCGCTGAAGTTCATGTGGCAATCGCCAGTCCCGCTCTCAAATATCCTTGCTTTTATGGCATTGATAT
TCAAGATCGTGACGAACTCATCGCAGCTACACACACGACTGATGAAATCAGAGAAGCTATCGGAGCAGATAGTCTGACCT
ATCTCAGCCAGACAGGACTTGTCGAGGCGATTGGTCATGATAAACTTTGCTTGTCATATTTTGATGGCGAATATCCAACG
CCGCTTTATGATTATGAAGCTGACTATTTGGAATCGTTAGCGAAAAATAGCGTGAAATGA

Upstream 100 bases:

>100_bases
TAACTTGAAAAAATTAAAAAAGTCCTATTAGGACTTTTTATTTCCAAACAATATAGATAGAATTAAAATATAATAAAAGA
ATCGATAGAGGGAAAAAATT

Downstream 100 bases:

>100_bases
AAAACTAGAGTGCTCACTCTAGTTTTTTTAGCTAAATTCTAAAGCGATTATTTTAGATTACTCCTTTTAATATGTTAGGA
TAAGAAATAAAAGCAAGTGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MFGKEQTLDLRVLGKNSSNENSWWQQHPDDERSHLNEECGLFGVLGHPDAARLTYFGLHALQHRGQEGAGILVNNNGKLN
RHRGLGLVTEVFRDEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGALGLAHNGNLTNAQSLRCELEKSGAIF
SSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASETCAFDVV
GATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAQIDADIVIGVP
NSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLL
KDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQTGLVEAIGHDKLCLSYFDGEYPT
PLYDYEADYLESLAKNSVK

Sequences:

>Translated_499_residues
MFGKEQTLDLRVLGKNSSNENSWWQQHPDDERSHLNEECGLFGVLGHPDAARLTYFGLHALQHRGQEGAGILVNNNGKLN
RHRGLGLVTEVFRDEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGALGLAHNGNLTNAQSLRCELEKSGAIF
SSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASETCAFDVV
GATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAQIDADIVIGVP
NSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLL
KDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQTGLVEAIGHDKLCLSYFDGEYPT
PLYDYEADYLESLAKNSVK
>Mature_499_residues
MFGKEQTLDLRVLGKNSSNENSWWQQHPDDERSHLNEECGLFGVLGHPDAARLTYFGLHALQHRGQEGAGILVNNNGKLN
RHRGLGLVTEVFRDEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGALGLAHNGNLTNAQSLRCELEKSGAIF
SSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASETCAFDVV
GATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAQIDADIVIGVP
NSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLL
KDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQTGLVEAIGHDKLCLSYFDGEYPT
PLYDYEADYLESLAKNSVK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=463, Percent_Identity=41.036717062635, Blast_Score=342, Evalue=5e-94,
Organism=Escherichia coli, GI1788651, Length=489, Percent_Identity=38.241308793456, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=40.3726708074534, Blast_Score=325, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6323958, Length=495, Percent_Identity=34.7474747474747, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=41.010101010101, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=40.8366533864542, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24659604, Length=462, Percent_Identity=42.2077922077922, Blast_Score=354, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54732; Mature: 54732

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKEQTLDLRVLGKNSSNENSWWQQHPDDERSHLNEECGLFGVLGHPDAARLTYFGLHA
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
LQHRGQEGAGILVNNNGKLNRHRGLGLVTEVFRDEKDLEELTGSSAIGHVRYATAGSANI
HHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCC
NNIQPFQFEFHDGALGLAHNGNLTNAQSLRCELEKSGAIFSSNSDTEILMHLIRRSHHPE
CCCCCEEEEECCCEEEEEECCCCCCHHHHEEEECCCCCEEECCCCHHHHHHHHHHCCCHH
FMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASETCAFDVV
HHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCEEEEEECCCEEEEC
GATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKR
CHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCC
SGKILAQEAQIDADIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELR
CCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
EQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLLKDAGAAEVHVAIASPALKYP
HCCCEEEEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
CFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQTGLVEAIGHDKLCLSYFDGEYPT
EEEECCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEECCCCCCC
PLYDYEADYLESLAKNSVK
CCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFGKEQTLDLRVLGKNSSNENSWWQQHPDDERSHLNEECGLFGVLGHPDAARLTYFGLHA
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
LQHRGQEGAGILVNNNGKLNRHRGLGLVTEVFRDEKDLEELTGSSAIGHVRYATAGSANI
HHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCC
NNIQPFQFEFHDGALGLAHNGNLTNAQSLRCELEKSGAIFSSNSDTEILMHLIRRSHHPE
CCCCCEEEEECCCEEEEEECCCCCCHHHHEEEECCCCCEEECCCCHHHHHHHHHHCCCHH
FMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASETCAFDVV
HHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCEEEEEECCCEEEEC
GATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKR
CHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCC
SGKILAQEAQIDADIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELR
CCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
EQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLLKDAGAAEVHVAIASPALKYP
HCCCEEEEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
CFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQTGLVEAIGHDKLCLSYFDGEYPT
EEEECCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEECCCCCCC
PLYDYEADYLESLAKNSVK
CCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA