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The map label for this gene is ypcA

Identifier: 15673471

GI number: 15673471

Start: 1519866

End: 1521293

Strand: Reverse

Name: ypcA

Synonym: L119614

Alternate gene names: 15673471

Gene position: 1521293-1519866 (Counterclockwise)

Preceding gene: 15673472

Following gene: 15673470

Centisome position: 64.31

GC content: 35.85

Gene sequence:

>1428_bases
ATGGAATTAAAATTCCAAGAAAATTTTTTGTGGGGAGCAGCAACGAGTGGCCCACAAACTGAAGGACGTTTTAAAAAGCA
GCATGACAATGTTTTTGATTATGATTTTGACCATTATCAAGAACGTTTTTGGGGCGGAATTGGACCTGATACAGCCAGTA
ATTTTTTCAATGACTTTCGAGAAGATATAAAATTAATGAAGGCTGCTGGTCTAAAATCAATAAGGACAAGTATTCAATGG
TCGCGTTTGATAGATGATTTAGAAAAGAATACGGTAAATGAAAAGGCGGTTGATTTCTATAATGCTGTAATTAATGAATT
TATAGCGCAAGGGATTCGCCCGATGATTAATTTAAATCATTTTGATTTACCAGTTGAACTCTTAAAAACATATGGAGGCT
GGGAAAGTCCTCATGTTGTTGATTTATATGCAGGTTTTGCAGCAAAATGTTTTGCTGAATTCAGCGACCGAGTGAGTGAT
TGGTTTACTTTTAATGAGCCAATGGTTGTCGTTGAGTGTGCTTATCTATTAGGTTTCCATTATCCTGATATTGTTGATGG
AAAAAAAGCTGTTCAAGTGGCTTATCATTTGCAACTCGCCTCATCTCTTGCGATTGAACAATACCGAAAAATCAATAAAA
ATCCTGAAGGACGAATTGGAATCATTTTAAATTTAACTCCGTCCTATCCAGCAGCTGAAAAAATAGAAGATTTAAAAGCA
GCAGAAATGGCAGACCTTTGGCAAAATCGCCTCTTTTTAGATGCATCAATAAAGGGAGAATTTCCAGAAGAATTAGTCAG
CCTTTTACAAGAAGAAAATGTCTTGTGGCAGTCGGAAGCAGAAGATTTGAAACGGATTAAGGAAAACAAAGTGGACCTTT
TGGGAGTTAATTACTACCATCCGAGTCGAGTACAAGAACCTGAATATTCTAGTGATAGTTTGGCTCAAGATTGGCGTCCC
GACAAATATTACGCATCGTTCAATAAACGTGGGGTGCGGATGAATGCTGACAGAGGTTGGGAGATACATCCACAAACCAT
TTACGAAATTGCCAAGCGAATTCAAGAAGATTATGGAAATATTTCTTGGTTTATTTCTGAAAATGGGATGGGCGTAGAAA
ATGAAGAACATTTCAAAGATGAAGAGGGACAAATCCAAGATGATTATCGAATTATTTTTACAACAGAACATCTTTTTTGG
CTCCATAAAGCGATTCAAGAAGGCTCCAATTGTTTTGGTTACCATGTTTGGACACCAATTGATTGCTGGAGTTGGCGTAA
TTCATACAAAAATCGTTATGGTTTGATTAGTTTGAATATCCATACCCAAGTGAAAACTTTAAAAAAATCAGCTTATTGGT
ATAAAAATTTAACAGACTCATCACTTTTACAAGTTCCAGATTCAATTTTAGAAAAATACAATCAATGA

Upstream 100 bases:

>100_bases
TTTTACAAGTTTATTCTTTAAGTGTGACAAAAACTTGGCGTTTGAAAGTCGAAGAACAGTTAGCAGAGTAAAAAAGAAGA
GTTACTGAGAGGGGTGGAAA

Downstream 100 bases:

>100_bases
TTTCTGTTAAAATATAAGAAAATTTGGAGGAGAAATATGTCATTATTAACAATAGATATTGGTGGAACAAGCATTAAATA
TGCAAGATTTGCTGACGGAA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MELKFQENFLWGAATSGPQTEGRFKKQHDNVFDYDFDHYQERFWGGIGPDTASNFFNDFREDIKLMKAAGLKSIRTSIQW
SRLIDDLEKNTVNEKAVDFYNAVINEFIAQGIRPMINLNHFDLPVELLKTYGGWESPHVVDLYAGFAAKCFAEFSDRVSD
WFTFNEPMVVVECAYLLGFHYPDIVDGKKAVQVAYHLQLASSLAIEQYRKINKNPEGRIGIILNLTPSYPAAEKIEDLKA
AEMADLWQNRLFLDASIKGEFPEELVSLLQEENVLWQSEAEDLKRIKENKVDLLGVNYYHPSRVQEPEYSSDSLAQDWRP
DKYYASFNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGNISWFISENGMGVENEEHFKDEEGQIQDDYRIIFTTEHLFW
LHKAIQEGSNCFGYHVWTPIDCWSWRNSYKNRYGLISLNIHTQVKTLKKSAYWYKNLTDSSLLQVPDSILEKYNQ

Sequences:

>Translated_475_residues
MELKFQENFLWGAATSGPQTEGRFKKQHDNVFDYDFDHYQERFWGGIGPDTASNFFNDFREDIKLMKAAGLKSIRTSIQW
SRLIDDLEKNTVNEKAVDFYNAVINEFIAQGIRPMINLNHFDLPVELLKTYGGWESPHVVDLYAGFAAKCFAEFSDRVSD
WFTFNEPMVVVECAYLLGFHYPDIVDGKKAVQVAYHLQLASSLAIEQYRKINKNPEGRIGIILNLTPSYPAAEKIEDLKA
AEMADLWQNRLFLDASIKGEFPEELVSLLQEENVLWQSEAEDLKRIKENKVDLLGVNYYHPSRVQEPEYSSDSLAQDWRP
DKYYASFNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGNISWFISENGMGVENEEHFKDEEGQIQDDYRIIFTTEHLFW
LHKAIQEGSNCFGYHVWTPIDCWSWRNSYKNRYGLISLNIHTQVKTLKKSAYWYKNLTDSSLLQVPDSILEKYNQ
>Mature_475_residues
MELKFQENFLWGAATSGPQTEGRFKKQHDNVFDYDFDHYQERFWGGIGPDTASNFFNDFREDIKLMKAAGLKSIRTSIQW
SRLIDDLEKNTVNEKAVDFYNAVINEFIAQGIRPMINLNHFDLPVELLKTYGGWESPHVVDLYAGFAAKCFAEFSDRVSD
WFTFNEPMVVVECAYLLGFHYPDIVDGKKAVQVAYHLQLASSLAIEQYRKINKNPEGRIGIILNLTPSYPAAEKIEDLKA
AEMADLWQNRLFLDASIKGEFPEELVSLLQEENVLWQSEAEDLKRIKENKVDLLGVNYYHPSRVQEPEYSSDSLAQDWRP
DKYYASFNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGNISWFISENGMGVENEEHFKDEEGQIQDDYRIIFTTEHLFW
LHKAIQEGSNCFGYHVWTPIDCWSWRNSYKNRYGLISLNIHTQVKTLKKSAYWYKNLTDSSLLQVPDSILEKYNQ

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=480, Percent_Identity=26.6666666666667, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI110681710, Length=489, Percent_Identity=25.5623721881391, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI13273313, Length=464, Percent_Identity=26.7241379310345, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI28376633, Length=459, Percent_Identity=24.1830065359477, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI24497614, Length=482, Percent_Identity=23.4439834024896, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI2367174, Length=490, Percent_Identity=29.1836734693878, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789070, Length=481, Percent_Identity=30.3534303534304, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI2367270, Length=484, Percent_Identity=28.3057851239669, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17552856, Length=472, Percent_Identity=27.5423728813559, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17539390, Length=474, Percent_Identity=27.6371308016878, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356577, Length=488, Percent_Identity=23.155737704918, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55463; Mature: 55463

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKFQENFLWGAATSGPQTEGRFKKQHDNVFDYDFDHYQERFWGGIGPDTASNFFNDFR
CCCEECCCEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
EDIKLMKAAGLKSIRTSIQWSRLIDDLEKNTVNEKAVDFYNAVINEFIAQGIRPMINLNH
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCC
FDLPVELLKTYGGWESPHVVDLYAGFAAKCFAEFSDRVSDWFTFNEPMVVVECAYLLGFH
CCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHHHHHCC
YPDIVDGKKAVQVAYHLQLASSLAIEQYRKINKNPEGRIGIILNLTPSYPAAEKIEDLKA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AEMADLWQNRLFLDASIKGEFPEELVSLLQEENVLWQSEAEDLKRIKENKVDLLGVNYYH
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCC
PSRVQEPEYSSDSLAQDWRPDKYYASFNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGN
CCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCEECCCCCCEECHHHHHHHHHHHHHHCCC
ISWFISENGMGVENEEHFKDEEGQIQDDYRIIFTTEHLFWLHKAIQEGSNCFGYHVWTPI
EEEEEECCCCCCCCHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCC
DCWSWRNSYKNRYGLISLNIHTQVKTLKKSAYWYKNLTDSSLLQVPDSILEKYNQ
CHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHCC
>Mature Secondary Structure
MELKFQENFLWGAATSGPQTEGRFKKQHDNVFDYDFDHYQERFWGGIGPDTASNFFNDFR
CCCEECCCEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
EDIKLMKAAGLKSIRTSIQWSRLIDDLEKNTVNEKAVDFYNAVINEFIAQGIRPMINLNH
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCC
FDLPVELLKTYGGWESPHVVDLYAGFAAKCFAEFSDRVSDWFTFNEPMVVVECAYLLGFH
CCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHHHHHCC
YPDIVDGKKAVQVAYHLQLASSLAIEQYRKINKNPEGRIGIILNLTPSYPAAEKIEDLKA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AEMADLWQNRLFLDASIKGEFPEELVSLLQEENVLWQSEAEDLKRIKENKVDLLGVNYYH
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCC
PSRVQEPEYSSDSLAQDWRPDKYYASFNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGN
CCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCEECCCCCCEECHHHHHHHHHHHHHHCCC
ISWFISENGMGVENEEHFKDEEGQIQDDYRIIFTTEHLFWLHKAIQEGSNCFGYHVWTPI
EEEEEECCCCCCCCHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCC
DCWSWRNSYKNRYGLISLNIHTQVKTLKKSAYWYKNLTDSSLLQVPDSILEKYNQ
CHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]