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The map label for this gene is trpB

Identifier: 15673445

GI number: 15673445

Start: 1494714

End: 1495922

Strand: Reverse

Name: trpB

Synonym: L0049

Alternate gene names: 15673445

Gene position: 1495922-1494714 (Counterclockwise)

Preceding gene: 15673446

Following gene: 15673444

Centisome position: 63.24

GC content: 40.94

Gene sequence:

>1209_bases
ATGACCTACAACCAACCTAACAACAAAGGATTTTACGGCCAATTCGGGGGCCAATTCGTACCTGAGACACTAATGACAGC
AGTAAAACAATTAGAAGAAGCCTACGTAGATAGTAAAAAAGACCCTCTCTTTCAAGCAGAACTTAAAGAATTACTTAAAG
ACTATGTTGGACGAGAAAACCCACTCTATTATGCAAAACGCTTAACAGAATATGCGGGCGGAGCAAAAATTTATCTTAAA
AGAGAAGACCTAAACCATACAGGAGCACACAAAATTAACAATGCCCTCGGACAAGTCCTCCTTGCCAAAAAAATGGGAAA
AAATAAAGTCATTGCTGAAACAGGTGCAGGCCAACACGGTGTCGCAAGCGCAACCGCGGCTGCCCTCTTTGGCATGGAAT
GTACGATTTATATGGGTGAAGAAGACGTTAAAAGACAATCTCTCAATGTCTTTCGCATGGAATTACTCGGGGCAAAAGTT
CATTCAGTAACTGATGGTTCACGCGTACTTAAAGATGCGGTTAATGCAGCACTTAGAGCATGGGTTGCTCAAGTTGAAGA
TACGCATTATGTAATGGGCTCAGTTCTTGGACCACATCCATTTCCACAAATTGTGCGTGATTATCAAGCTGTTATTGGAC
AGGAAGCGCGTGCCCAATTTTTAGAAAAAGAAAATAAACTTCCAGATGCTTTAGTAGCTTGTGTCGGTGGAGGTTCAAAT
TCTATGGGACTTTTTTATCCCTTCGTTAATGATGAATCAGTTGCCATGTATGGTGTTGAAGCCGCTGGCCTTGGGATTGA
TACACCACATCATGCGGCAACAATTACTAAAGGCCGCCCCGGTGTTCTTCACGGAACACTCATGGATGTCCTTCAAGATG
AAAATGGTCAAATGTTAGAAGCCTTTAGTATTTCAGCCGGTTTAGACTATCCAGGAATCGGACCAGAACACTCTTATTTC
AATGCTGTTGGACGAGCAAAATATGTTGATATTACAGATGAAGAAGCACTTGAAGGTTTTAAAATCTTATCTAGAACTGA
AGGAATTATCCCAGCACTAGAAAGTTCTCATGCTATCGCCTATGCAGTCAAATTAGCAAAAGAATTAGGAGCAGATAAAT
CAATGATTGTTTGTCTTTCAGGACGTGGAGATAAGGATGTGGTTCAAGTTAAAGAACGACTTGAAGCAGAAAAAGAGGTG
AAAAAATGA

Upstream 100 bases:

>100_bases
AAGTTAGATATTGGCAAGAAACTTGGTATGATTCAATAAAATATGGAATTTTAAGAGAAGAACTAAAAAAATAAATAAAA
AAAATCAAAGGAGCAACAAC

Downstream 100 bases:

>100_bases
AAACTTTACAAATGACTTTAAGCAATAAAAAAAATAATTTTATTCCTTATATCATGGCTGGCGACCATGAAAAAGGCTTA
GAAGGTCTTAAAGAAACCAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGRENPLYYAKRLTEYAGGAKIYLK
REDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHGVASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKV
HSVTDGSRVLKDAVNAALRAWVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSN
SMGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLEAFSISAGLDYPGIGPEHSYF
NAVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIAYAVKLAKELGADKSMIVCLSGRGDKDVVQVKERLEAEKEV
KK

Sequences:

>Translated_402_residues
MTYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGRENPLYYAKRLTEYAGGAKIYLK
REDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHGVASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKV
HSVTDGSRVLKDAVNAALRAWVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSN
SMGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLEAFSISAGLDYPGIGPEHSYF
NAVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIAYAVKLAKELGADKSMIVCLSGRGDKDVVQVKERLEAEKEV
KK
>Mature_401_residues
TYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGRENPLYYAKRLTEYAGGAKIYLKR
EDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHGVASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKVH
SVTDGSRVLKDAVNAALRAWVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSNS
MGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLEAFSISAGLDYPGIGPEHSYFN
AVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIAYAVKLAKELGADKSMIVCLSGRGDKDVVQVKERLEAEKEVK
K

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=392, Percent_Identity=55.1020408163265, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=57.8125, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_LACLA (Q01998)

Other databases:

- EMBL:   M87483
- EMBL:   AE005176
- PIR:   S35129
- RefSeq:   NP_267619.1
- ProteinModelPortal:   Q01998
- SMR:   Q01998
- GeneID:   1115120
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0049
- NMPDR:   fig|272623.1.peg.1504
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   LLAC272623:L0049-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43746; Mature: 43615

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGREN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKRLTEYAGGAKIYLKREDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
VASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKVHSVTDGSRVLKDAVNAALRA
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSN
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHEEECCCCC
SMGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLE
CCEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEE
AFSISAGLDYPGIGPEHSYFNAVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIA
EEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
YAVKLAKELGADKSMIVCLSGRGDKDVVQVKERLEAEKEVKK
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLFQAELKELLKDYVGREN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKRLTEYAGGAKIYLKREDLNHTGAHKINNALGQVLLAKKMGKNKVIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
VASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKVHSVTDGSRVLKDAVNAALRA
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARAQFLEKENKLPDALVACVGGGSN
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHEEECCCCC
SMGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATITKGRPGVLHGTLMDVLQDENGQMLE
CCEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEE
AFSISAGLDYPGIGPEHSYFNAVGRAKYVDITDEEALEGFKILSRTEGIIPALESSHAIA
EEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
YAVKLAKELGADKSMIVCLSGRGDKDVVQVKERLEAEKEVKK
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471