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The map label for this gene is trpA

Identifier: 15673444

GI number: 15673444

Start: 1493956

End: 1494717

Strand: Reverse

Name: trpA

Synonym: L0048

Alternate gene names: 15673444

Gene position: 1494717-1493956 (Counterclockwise)

Preceding gene: 15673445

Following gene: 15673443

Centisome position: 63.19

GC content: 35.04

Gene sequence:

>762_bases
ATGAAAACTTTACAAATGACTTTAAGCAATAAAAAAAATAATTTTATTCCTTATATCATGGCTGGCGACCATGAAAAAGG
CTTAGAAGGTCTTAAAGAAACCATTCAACTGCTTGAGCAAGCTGGGAGTTCCGCTATTGAAATTGGCGTTCCATTTTCAG
ATCCGGTTGCTGATGGTCCAGTCATCGAACAAGCAGGTTTGCGTGCGTTAGCAAGAAATGTATCACTTTCAAGTATTCTT
GAAACCTTAAAAACAATTGATACAAAAGTTCCTCTAGTAATTATGACCTATTTCAATCCCGTTTATCAGTTTGGAATTGA
AAAGTTTGTTGCAGCTCTTGAAAAAACACCAGTTAAAGGCCTTATCATTCCTGATTTGCCTAAAGAACATGAGGACTATA
TCAAACCATTTATCAATGATAAAGATATCTGTTTAGTTCCTCTGGTCTCATTAACCACGCCACTTTCTCGGCAAAAAGAA
CTTGTAGCCGATGCTGAAGGATTTATCTATGCCGTTGCAATAAATGGAGTAACTGGGAAAGAAAATGCTTATAGTAACCA
GCTTGACCAACATTTAAAAGCGTTATCTTCATTAACGGATGTTCCTGTTTTGACAGGATTTGGAATTTCTACATTATCTG
ATGTGGACCGTTTTAATAAAGTGTCCTCAGGAGTTATTGTTGGTTCAAAAATTGTTCGTGATTTACATGAAGGTAAAGAA
AACGAAGTTATTAAATTTATTGAAAACGCAATCAATTTTTAA

Upstream 100 bases:

>100_bases
TAGGAGCAGATAAATCAATGATTGTTTGTCTTTCAGGACGTGGAGATAAGGATGTGGTTCAAGTTAAAGAACGACTTGAA
GCAGAAAAAGAGGTGAAAAA

Downstream 100 bases:

>100_bases
GATAATTGAAAAATTAATAAAACCATATATCCTTGACAGTCCACTTTACAAATGGTATACTTTTATTAAATAAAACGGTT
ACAAAAAATTGGATAGTGAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALARNVSLSSIL
ETLKTIDTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKGLIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKE
LVADAEGFIYAVAINGVTGKENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRFNKVSSGVIVGSKIVRDLHEGKE
NEVIKFIENAINF

Sequences:

>Translated_253_residues
MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALARNVSLSSIL
ETLKTIDTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKGLIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKE
LVADAEGFIYAVAINGVTGKENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRFNKVSSGVIVGSKIVRDLHEGKE
NEVIKFIENAINF
>Mature_253_residues
MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALARNVSLSSIL
ETLKTIDTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKGLIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKE
LVADAEGFIYAVAINGVTGKENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRFNKVSSGVIVGSKIVRDLHEGKE
NEVIKFIENAINF

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=209, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=264, Percent_Identity=33.7121212121212, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LACLA (Q01997)

Other databases:

- EMBL:   M87483
- EMBL:   AE005176
- PIR:   S35130
- RefSeq:   NP_267618.1
- ProteinModelPortal:   Q01997
- SMR:   Q01997
- GeneID:   1115119
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0048
- NMPDR:   fig|272623.1.peg.1503
- HOGENOM:   HBG391690
- OMA:   VILMTYY
- ProtClustDB:   PRK13111
- BioCyc:   LLAC272623:L0048-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27685; Mature: 27685

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSSAIEIGVPFSDPVADGP
CCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VIEQAGLRALARNVSLSSILETLKTIDTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
LIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKELVADAEGFIYAVAINGVTGK
EECCCCCCCHHHHHHHCCCCCCCCEEEHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCC
ENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRFNKVSSGVIVGSKIVRDLHEGKE
CHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCH
NEVIKFIENAINF
HHHHHHHHHHHCC
>Mature Secondary Structure
MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSSAIEIGVPFSDPVADGP
CCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VIEQAGLRALARNVSLSSILETLKTIDTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
LIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKELVADAEGFIYAVAINGVTGK
EECCCCCCCHHHHHHHCCCCCCCCEEEHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCC
ENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRFNKVSSGVIVGSKIVRDLHEGKE
CHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCH
NEVIKFIENAINF
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471