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The map label for this gene is rluC

Identifier: 15673334

GI number: 15673334

Start: 1390135

End: 1390692

Strand: Reverse

Name: rluC

Synonym: L189881

Alternate gene names: 15673334

Gene position: 1390692-1390135 (Counterclockwise)

Preceding gene: 15673335

Following gene: 15673330

Centisome position: 58.79

GC content: 34.41

Gene sequence:

>558_bases
ATGAACTTTTGCAAAAAAATTATGATTATATTATTTAATAAACCTTATGATGTACTAACAAAATTTACTGACGGAGAGGG
TCGGAAAACTTTAAAAGATTATATTAAAATACCAAATGTTTATGCTGCTGGAAGATTGGATAAAGATAGTGAAGGTCTAC
TTTTACTGACCGATGATGGAAAGCTCAATCATTTACTGACAGATCCAAAATCAAAATCTTACAAGACTTATTTGGTTCAA
GTAGAGGGAGAATTTTCGCCAGAAGCAGCGCAAAAGTTGCAAAAAGGTCTTCTATTAAAAGATGGTAAAACATTGCCTGC
GAAAGTAAAATTAATTGATGAACCAGATTGGCTTTGGGAACGAACTCCACCAATTCGTGAACGAAAGGCAATTCCGACTA
GCTGGGTTGAAATTTCAATCAAAGAAGGAAAAAATCGGCAAATTAGAAGAATGACGGCAGCGGTCGGTTTCCCAACACTT
CGGTTAATTCGTTGGTCAATTGGTCAATATTCACTGGGAAATCTAAAATCTGGGGAATATAAAATTATTCAAAAATAA

Upstream 100 bases:

>100_bases
AAGCTCATCAATAATAGCCAAAGTACAAACTAAAACTTTGTTTGTAAGCTAATAATAATTTTTTTTGGTAAAATAATTAA
TAGAAACAGATTTGAGAAAG

Downstream 100 bases:

>100_bases
AAAGGAATCATTTTGTGATTCCTTTTTATTTAGAAAGAACGTCCGCCACCAGAACCGTCACTGAAATTATTGTTATCAGA
AGAAGGACGAGGGATAATTC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNFCKKIMIILFNKPYDVLTKFTDGEGRKTLKDYIKIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSYKTYLVQ
VEGEFSPEAAQKLQKGLLLKDGKTLPAKVKLIDEPDWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTL
RLIRWSIGQYSLGNLKSGEYKIIQK

Sequences:

>Translated_185_residues
MNFCKKIMIILFNKPYDVLTKFTDGEGRKTLKDYIKIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSYKTYLVQ
VEGEFSPEAAQKLQKGLLLKDGKTLPAKVKLIDEPDWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTL
RLIRWSIGQYSLGNLKSGEYKIIQK
>Mature_185_residues
MNFCKKIMIILFNKPYDVLTKFTDGEGRKTLKDYIKIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSYKTYLVQ
VEGEFSPEAAQKLQKGLLLKDGKTLPAKVKLIDEPDWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTL
RLIRWSIGQYSLGNLKSGEYKIIQK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=61.1428571428571, Blast_Score=229, Evalue=8e-62,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=34.2696629213483, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787524, Length=169, Percent_Identity=32.5443786982249, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790453, Length=179, Percent_Identity=27.3743016759777, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 21224; Mature: 21224

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFCKKIMIILFNKPYDVLTKFTDGEGRKTLKDYIKIPNVYAAGRLDKDSEGLLLLTDDG
CCHHHEEEEEEECCCHHHHEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCC
KLNHLLTDPKSKSYKTYLVQVEGEFSPEAAQKLQKGLLLKDGKTLPAKVKLIDEPDWLWE
CCCEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCC
RTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSIGQYSLGNLKSGEY
CCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCE
KIIQK
EEEEC
>Mature Secondary Structure
MNFCKKIMIILFNKPYDVLTKFTDGEGRKTLKDYIKIPNVYAAGRLDKDSEGLLLLTDDG
CCHHHEEEEEEECCCHHHHEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCC
KLNHLLTDPKSKSYKTYLVQVEGEFSPEAAQKLQKGLLLKDGKTLPAKVKLIDEPDWLWE
CCCEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCC
RTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSIGQYSLGNLKSGEY
CCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCE
KIIQK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]