Click here to switch to the map view.

The map label for this gene is yniG

Identifier: 15673330

GI number: 15673330

Start: 1385072

End: 1386436

Strand: Reverse

Name: yniG

Synonym: L184818

Alternate gene names: 15673330

Gene position: 1386436-1385072 (Counterclockwise)

Preceding gene: 15673334

Following gene: 15673329

Centisome position: 58.61

GC content: 37.95

Gene sequence:

>1365_bases
ATGAATAAAAACAACCATTTCCACTTATTAGGGGCAGTGATTGCTACAGGGATTTTGTCATTTTCTGGCGTTTTGATTGA
AACGGCGATGAATGTGACTTTTCCAACGCTGATTAATGAGTTTGGACTTTCTACTTCAAAAATACAATGGGTAACGACGA
TTTATCTGCTTGTGATTGCGATTACGATTCCGCTCTCTTCTTATTTTAATGAGCGATTTTCAGCAAGAAAACTTTTCTTG
GTCGCTAATCTTATCTTTTTAGTTGGGGTTTTGACCAACTGTTTCTCACCAAATTTTGCCATGCTATTATTTGGCCGACT
TTTACAAGGGGTGGGAACCGGGATTGGTTTACCTCTGATGTTCCACCTGATTTTGACTAAAGCACCACTTCATAAAAGAG
GAATGATGATGGGGATTGGGACTTTAACGACTTCCATCGCTCCGGCTATTGGCCCAACTTATGGAGGAATTATTTCTAAT
TCGCTTGATTGGCGTTATATCTATATTTTCCTTTTACCTTTGGTGGTAATTTCTTTAGTGCTTGGTTTGGCTTGTCTTCC
AAGAGAAGGGGAAAAAACGCCCAAGAAATTGGCGCTTCGTCCAGTTATTGCATTATCAATCATGTTCTTTGCTTTCATTT
CGGCTCTAAGTGCAGAGCATTTAATGACTTTCGCCTTACTCTTTATTGTAGGATTGGTTGGAGCTTTCTTGTTTGTTCAA
TTCAACCGCAAAGAAAGTTTAATTGATTTAGGAATTTTGAAAAATCACCGATTTGTGGCGCTAATTTTCAGTCTTTTAGT
ATATCAAGCTTTGCTATTAGGTCTATCTTTTGTTTTACCAAGTTTTATTCAAGTTTCTGCGGGATTTAGTTCATCAGTGG
CGGGCCTATTTATGTTTTCTGGAGCTTTGATTGGCGCAGTTTTAGCACCAATTTCTGGGAAAGTTTTGGACCAAATTGGT
GCCAGAAAACCAATTACGACTGGTTTGATTTTAGCAGCCCTTGGTCTTGCTTTGATCTTCGTCTTTTTACCAACAAAAAG
TCTTGCTTTACTCTTGGGGGCACACATTGTCTTGATGTTAGGTCTTGGGATTTCATATAGTAACTTAATGACTTGTAGCT
TGAGCACTTTAGCTACTGACCAACTTTCTGATGGAAATGCTTTGGTCAATACTTTGCAACAATTTATCGGTGCAGTAGCA
ACAGCAGTTGTCGCAACGACACTTTCAATTTTCCAAGGACTCAATGGCTTCAAAGTTGGAACAAGTCATGGAACTTCAAT
TATTTTAGCTCTATTTTTCATCTTGATTATCGTGAGTTTAATTGTCAGTTTCCCAAATTTGAAAAAAATTAGAGCCAATC
ATTAA

Upstream 100 bases:

>100_bases
TACAGATAACATAAATTATGTTGAATTATACAGAGGCGAAATTATTATTTTAGGATAATAATTTCGCCTCTTTTTGTTTA
GTTTTAGGAGATTTTATTGC

Downstream 100 bases:

>100_bases
AAAAATCTTTGACGAATGACACTTTTTTCGTTATAATATTTTAAAACCTTTAACTAAGTCCAGAGAGGCTTGCAAGGATA
CGGAAAATTTAATTTGGTTT

Product: drug-export protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLINEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSARKLFL
VANLIFLVGVLTNCFSPNFAMLLFGRLLQGVGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIISN
SLDWRYIYIFLLPLVVISLVLGLACLPREGEKTPKKLALRPVIALSIMFFAFISALSAEHLMTFALLFIVGLVGAFLFVQ
FNRKESLIDLGILKNHRFVALIFSLLVYQALLLGLSFVLPSFIQVSAGFSSSVAGLFMFSGALIGAVLAPISGKVLDQIG
ARKPITTGLILAALGLALIFVFLPTKSLALLLGAHIVLMLGLGISYSNLMTCSLSTLATDQLSDGNALVNTLQQFIGAVA
TAVVATTLSIFQGLNGFKVGTSHGTSIILALFFILIIVSLIVSFPNLKKIRANH

Sequences:

>Translated_454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLINEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSARKLFL
VANLIFLVGVLTNCFSPNFAMLLFGRLLQGVGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIISN
SLDWRYIYIFLLPLVVISLVLGLACLPREGEKTPKKLALRPVIALSIMFFAFISALSAEHLMTFALLFIVGLVGAFLFVQ
FNRKESLIDLGILKNHRFVALIFSLLVYQALLLGLSFVLPSFIQVSAGFSSSVAGLFMFSGALIGAVLAPISGKVLDQIG
ARKPITTGLILAALGLALIFVFLPTKSLALLLGAHIVLMLGLGISYSNLMTCSLSTLATDQLSDGNALVNTLQQFIGAVA
TAVVATTLSIFQGLNGFKVGTSHGTSIILALFFILIIVSLIVSFPNLKKIRANH
>Mature_454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLINEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSARKLFL
VANLIFLVGVLTNCFSPNFAMLLFGRLLQGVGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIISN
SLDWRYIYIFLLPLVVISLVLGLACLPREGEKTPKKLALRPVIALSIMFFAFISALSAEHLMTFALLFIVGLVGAFLFVQ
FNRKESLIDLGILKNHRFVALIFSLLVYQALLLGLSFVLPSFIQVSAGFSSSVAGLFMFSGALIGAVLAPISGKVLDQIG
ARKPITTGLILAALGLALIFVFLPTKSLALLLGAHIVLMLGLGISYSNLMTCSLSTLATDQLSDGNALVNTLQQFIGAVA
TAVVATTLSIFQGLNGFKVGTSHGTSIILALFFILIIVSLIVSFPNLKKIRANH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=24.9382716049383, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=24.9388753056235, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=22.636815920398, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081983, Length=314, Percent_Identity=25.4777070063694, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788392, Length=442, Percent_Identity=23.7556561085973, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48694; Mature: 48694

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]