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The map label for this gene is thiM

Identifier: 15673246

GI number: 15673246

Start: 1295064

End: 1295816

Strand: Reverse

Name: thiM

Synonym: L94809

Alternate gene names: 15673246

Gene position: 1295816-1295064 (Counterclockwise)

Preceding gene: 15673247

Following gene: 15673245

Centisome position: 54.78

GC content: 35.86

Gene sequence:

>753_bases
ATGTCAATTTTAGAAAAGATTCAAGAAAAACAACCACTTATCTTAAATTTAGCGAATTTTGTAACTCCACAAAGAGTTGC
GGATGCGATTAGTTTTGCTGGTGGTTCACCATTAATGACTGCGGAAATTTCAGAAAGTGAAACTTTAGTTGAAATTGCAG
ATGCTGTAGTGGTTAATATTGGAACCATTTCAGAAAAGGATTATCCGCTTTTTTTGACTATTTGTCAGTTAGCAAATCAA
AAGCACAAACCTTTAATTTTGGACCCAGTTGCTGTCAATGTCCCTTTTAGAGCAAATTTTGTCAAAAGATTAATGCAAGA
GGTGAAATTTGATATCATTCGTGGGAATTCAGCGGAAATTGCTTGGTTTGCTGATAAAGAAAGCTTGAACAAAGGAATTG
ATGCACTTGAGTCAAATTTAGAAGTTGAGCACGCGCGAGTTGCTGCTCAAAAAACAGGGGCAGTCATTATTCAAACTGGA
AAAGTTGATGTTATTTCTAATGGGTTTGAGGAACTATTTGTTGAAACTGATAGTCCACTTTTTAAAATTAATGTTGGCTG
TGGGGATATGTTGTCAGCAATTGTTGGAACTTTTGCGGCTGTATCTGATGATTTATTTAAGGCGGCTTATGAAGCGACAG
AATTCTTCGGTGAATCTGGTGTGAAAGCTACTGAGCAAGTAGAAAATCTACCAGGAAATTTTGTGAATCGTTTTCTTGAT
AAAATTTATCAAGCGACGCAAGAGGTTATATAA

Upstream 100 bases:

>100_bases
GAATGTGTCCGAGTTTTAATTGTATTTTCTTTATGAAAATCTTTTAGCTTACTTTCTTATCATACTAGAGCCCTCCTTGT
GTTAATAAAGGAGTTTTTTG

Downstream 100 bases:

>100_bases
GGGAGAAAAAGAAATGATTGATGTTTCTAAAGTTGTGACAATCGCTGGAATTGATTCTTCTGGTGGGGCTGGTATTAATG
CTGACCTAAAAACTTTTCAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSILEKIQEKQPLILNLANFVTPQRVADAISFAGGSPLMTAEISESETLVEIADAVVVNIGTISEKDYPLFLTICQLANQ
KHKPLILDPVAVNVPFRANFVKRLMQEVKFDIIRGNSAEIAWFADKESLNKGIDALESNLEVEHARVAAQKTGAVIIQTG
KVDVISNGFEELFVETDSPLFKINVGCGDMLSAIVGTFAAVSDDLFKAAYEATEFFGESGVKATEQVENLPGNFVNRFLD
KIYQATQEVI

Sequences:

>Translated_250_residues
MSILEKIQEKQPLILNLANFVTPQRVADAISFAGGSPLMTAEISESETLVEIADAVVVNIGTISEKDYPLFLTICQLANQ
KHKPLILDPVAVNVPFRANFVKRLMQEVKFDIIRGNSAEIAWFADKESLNKGIDALESNLEVEHARVAAQKTGAVIIQTG
KVDVISNGFEELFVETDSPLFKINVGCGDMLSAIVGTFAAVSDDLFKAAYEATEFFGESGVKATEQVENLPGNFVNRFLD
KIYQATQEVI
>Mature_249_residues
SILEKIQEKQPLILNLANFVTPQRVADAISFAGGSPLMTAEISESETLVEIADAVVVNIGTISEKDYPLFLTICQLANQK
HKPLILDPVAVNVPFRANFVKRLMQEVKFDIIRGNSAEIAWFADKESLNKGIDALESNLEVEHARVAAQKTGAVIIQTGK
VDVISNGFEELFVETDSPLFKINVGCGDMLSAIVGTFAAVSDDLFKAAYEATEFFGESGVKATEQVENLPGNFVNRFLDK
IYQATQEVI

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=30.241935483871, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LACLA (Q9CG46)

Other databases:

- EMBL:   AE005176
- PIR:   H86782
- RefSeq:   NP_267420.1
- ProteinModelPortal:   Q9CG46
- SMR:   Q9CG46
- GeneID:   1114913
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L94809
- NMPDR:   fig|272623.1.peg.1299
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   LLAC272623:L94809-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27343; Mature: 27211

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: BINDING 39-39 BINDING 114-114 BINDING 159-159 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILEKIQEKQPLILNLANFVTPQRVADAISFAGGSPLMTAEISESETLVEIADAVVVNI
CCHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHEEE
GTISEKDYPLFLTICQLANQKHKPLILDPVAVNVPFRANFVKRLMQEVKFDIIRGNSAEI
CCCCCCCCHHHHHHHHHCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHEECCCCEE
AWFADKESLNKGIDALESNLEVEHARVAAQKTGAVIIQTGKVDVISNGFEELFVETDSPL
EEEECHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHCCHHHHHEECCCCE
FKINVGCGDMLSAIVGTFAAVSDDLFKAAYEATEFFGESGVKATEQVENLPGNFVNRFLD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHH
KIYQATQEVI
HHHHHHHHCC
>Mature Secondary Structure 
SILEKIQEKQPLILNLANFVTPQRVADAISFAGGSPLMTAEISESETLVEIADAVVVNI
CHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHEEE
GTISEKDYPLFLTICQLANQKHKPLILDPVAVNVPFRANFVKRLMQEVKFDIIRGNSAEI
CCCCCCCCHHHHHHHHHCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHEECCCCEE
AWFADKESLNKGIDALESNLEVEHARVAAQKTGAVIIQTGKVDVISNGFEELFVETDSPL
EEEECHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHCCHHHHHEECCCCE
FKINVGCGDMLSAIVGTFAAVSDDLFKAAYEATEFFGESGVKATEQVENLPGNFVNRFLD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHH
KIYQATQEVI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471