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The map label for this gene is glpK

Identifier: 15673228

GI number: 15673228

Start: 1271961

End: 1273457

Strand: Reverse

Name: glpK

Synonym: L0014

Alternate gene names: 15673228

Gene position: 1273457-1271961 (Counterclockwise)

Preceding gene: 15673229

Following gene: 15673227

Centisome position: 53.83

GC content: 42.89

Gene sequence:

>1497_bases
ATGACAGAAACTTCTTACATTATGGCAATTGACCAAGGTACAACAAGCTCAAGAGCTATTATATTTGATAAACATGGTAA
ACATATCGGCAGCTCCCAAAAGGAATTTACACAGTACTTTCCTAAAGAAGGTTGGGTGGAACATGATGCCAACGAAATTT
GGAACTCTGTTCAGTCTGTTATCGCAGGGGCTTTTATCGAGTCGGGGATTAAGCCTGTACAGGTTGCGGGTATCGGTATT
ACTAACCAACGTGAAACTACCATTATATGGGACAAGAAAACAGGAAAACCAATCTATCATGCTATTGTTTGGCAATCTCG
TCAATCTGCCGATATTGCCAATGGTCTTAAAGAAGCAGGACATGAGGAACTCTTCCATAAAAAGACAGGACTTATTGTCG
ATTCATATTTTTCAGCCACAAAAATTCGTTGGATCCTTGACCATGTCGAAGGAGCACAAGAGCGTGCAGAACGCGGAGAG
CTCCTCTTTGGAACCATCGATACATGGCTCTTGTGGAAGTTGACAGATGGTGATGCACACTATACTGACTATTCTAATGC
CAGCCGTACCATGCTCTACAACATTCATGAGTTGAAATGGGACGAAGAAATCCTGAAAATTCTTAATATCCCAGCTTCAA
TGCTTCCAGAAGTCAAGTCCAACTCAGAAGTTTACGGCGTGACTAAAGGTTTCCATTTCTTTGGCTCTGAAGTGCCAATC
TCAGGTATGGCAGGGGACCAGCAAGCTGCATTATTTGGTCAAATGGCCTTTGAACCTGGAATGATTAAAAATACCTATGG
TACAGGTTCGTTTATCGTCATGAACACAGGTGAGAAACCACATATTTCGAAAAATAATATGCTTACAACGATTGGTTATG
GCATTAATGGAAAAGTTTATTACGCCTTAGAAGGAAGCATCTTTGTTGCGGGGTCCTCTATCCAATGGTTACGTGATGGT
TTGAAAATGTTGGAACGTGCCAGTGATTCCGAAGAAGTGGCACTCCAGTCTAGGAGTCAAGATGAAGTTTATGTGGTGCC
AGCTTTTGTCGGCCTTGGTGCTCCGTATTGGGACCAAGAGGCGCGTGGTGCGATGTTTGGCTTGACACGTGGAACAACAA
AAGAAGATATTGTGAAAGCAACCTTACAAGGGATAGCTTATCAAGTTCGTGATGTAGTCAGCACCATGAAAGAAGATACA
GGAATTGATATGTCTGTGCTCAAGGTAGACGGTGGCGCTGCAAACAATGAATATTTGATGCAGTTCCAAGCTGATATCTT
GAATATCCCAATCCAACGTGCGGGAGATTTGGAAACGACAGCACTTGGTGCAGCCTTCCTTGCAGGACTTGCCGTTGGTT
TCTGGAAAGACTTAGAAGAGCTTAAAGAATCCTATGAACCGGGCAAAATTTTCCAAACTCAGATGCAAGAAGAACGTAGA
GAAGAACTCTATGCCGGTTGGAAAAAAGCCGTGAATGCGACACGTGCTTTCAAATAA

Upstream 100 bases:

>100_bases
CTGCCAATTTTTTTTTGAATTTGATAAAAAATGAAAGTTTCTATTCGTTTTAAAATGCTATAATATAAATCATAGAAAAC
GTTTACAAAGGAGAAACAAC

Downstream 100 bases:

>100_bases
TAATCAATCAGACAGTAATCTAAAAGGAGAATAGAAATGGCTTTTTCTAAAAAAACAAGACAAGAAGCAATCACAAAAAT
TCAAAATGAAGAGATGGATT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTETSYIMAIDQGTTSSRAIIFDKHGKHIGSSQKEFTQYFPKEGWVEHDANEIWNSVQSVIAGAFIESGIKPVQVAGIGI
TNQRETTIIWDKKTGKPIYHAIVWQSRQSADIANGLKEAGHEELFHKKTGLIVDSYFSATKIRWILDHVEGAQERAERGE
LLFGTIDTWLLWKLTDGDAHYTDYSNASRTMLYNIHELKWDEEILKILNIPASMLPEVKSNSEVYGVTKGFHFFGSEVPI
SGMAGDQQAALFGQMAFEPGMIKNTYGTGSFIVMNTGEKPHISKNNMLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDG
LKMLERASDSEEVALQSRSQDEVYVVPAFVGLGAPYWDQEARGAMFGLTRGTTKEDIVKATLQGIAYQVRDVVSTMKEDT
GIDMSVLKVDGGAANNEYLMQFQADILNIPIQRAGDLETTALGAAFLAGLAVGFWKDLEELKESYEPGKIFQTQMQEERR
EELYAGWKKAVNATRAFK

Sequences:

>Translated_498_residues
MTETSYIMAIDQGTTSSRAIIFDKHGKHIGSSQKEFTQYFPKEGWVEHDANEIWNSVQSVIAGAFIESGIKPVQVAGIGI
TNQRETTIIWDKKTGKPIYHAIVWQSRQSADIANGLKEAGHEELFHKKTGLIVDSYFSATKIRWILDHVEGAQERAERGE
LLFGTIDTWLLWKLTDGDAHYTDYSNASRTMLYNIHELKWDEEILKILNIPASMLPEVKSNSEVYGVTKGFHFFGSEVPI
SGMAGDQQAALFGQMAFEPGMIKNTYGTGSFIVMNTGEKPHISKNNMLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDG
LKMLERASDSEEVALQSRSQDEVYVVPAFVGLGAPYWDQEARGAMFGLTRGTTKEDIVKATLQGIAYQVRDVVSTMKEDT
GIDMSVLKVDGGAANNEYLMQFQADILNIPIQRAGDLETTALGAAFLAGLAVGFWKDLEELKESYEPGKIFQTQMQEERR
EELYAGWKKAVNATRAFK
>Mature_497_residues
TETSYIMAIDQGTTSSRAIIFDKHGKHIGSSQKEFTQYFPKEGWVEHDANEIWNSVQSVIAGAFIESGIKPVQVAGIGIT
NQRETTIIWDKKTGKPIYHAIVWQSRQSADIANGLKEAGHEELFHKKTGLIVDSYFSATKIRWILDHVEGAQERAERGEL
LFGTIDTWLLWKLTDGDAHYTDYSNASRTMLYNIHELKWDEEILKILNIPASMLPEVKSNSEVYGVTKGFHFFGSEVPIS
GMAGDQQAALFGQMAFEPGMIKNTYGTGSFIVMNTGEKPHISKNNMLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGL
KMLERASDSEEVALQSRSQDEVYVVPAFVGLGAPYWDQEARGAMFGLTRGTTKEDIVKATLQGIAYQVRDVVSTMKEDTG
IDMSVLKVDGGAANNEYLMQFQADILNIPIQRAGDLETTALGAAFLAGLAVGFWKDLEELKESYEPGKIFQTQMQEERRE
ELYAGWKKAVNATRAFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=45.7661290322581, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=44.5564516129032, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=44.5564516129032, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=44.2, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI88196792, Length=502, Percent_Identity=33.4661354581673, Blast_Score=310, Evalue=3e-84,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=57.2289156626506, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1790005, Length=469, Percent_Identity=23.8805970149254, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787789, Length=504, Percent_Identity=24.2063492063492, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789987, Length=473, Percent_Identity=22.8329809725159, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789168, Length=491, Percent_Identity=21.7922606924644, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=435, Percent_Identity=41.3793103448276, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=26.1036468330134, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=30.8970099667774, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=32.8519855595668, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI17864214, Length=501, Percent_Identity=40.5189620758483, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=38.1526104417671, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=38.1526104417671, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=38.1526104417671, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=38.1526104417671, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=38.1526104417671, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24654573, Length=453, Percent_Identity=40.1766004415011, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI19922058, Length=520, Percent_Identity=32.6923076923077, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24652871, Length=487, Percent_Identity=33.4702258726899, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24656367, Length=493, Percent_Identity=26.1663286004057, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI21357867, Length=509, Percent_Identity=24.5579567779961, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24656372, Length=344, Percent_Identity=27.3255813953488, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45552909, Length=296, Percent_Identity=24.3243243243243, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_LACLA (Q9CG64)

Other databases:

- EMBL:   AE005176
- PIR:   F86780
- RefSeq:   NP_267402.1
- ProteinModelPortal:   Q9CG64
- SMR:   Q9CG64
- GeneID:   1114895
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0014
- NMPDR:   fig|272623.1.peg.1281
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   LLAC272623:L0014-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55445; Mature: 55314

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 136-136 BINDING 246-246 BINDING 268-268 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETSYIMAIDQGTTSSRAIIFDKHGKHIGSSQKEFTQYFPKEGWVEHDANEIWNSVQSV
CCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESGIKPVQVAGIGITNQRETTIIWDKKTGKPIYHAIVWQSRQSADIANGLKEAG
HHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCC
HEELFHKKTGLIVDSYFSATKIRWILDHVEGAQERAERGELLFGTIDTWLLWKLTDGDAH
HHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEEEEEECCCCC
YTDYSNASRTMLYNIHELKWDEEILKILNIPASMLPEVKSNSEVYGVTKGFHFFGSEVPI
CCCCCCCCCEEEEEHHHHCHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCHHHCCCCCCC
SGMAGDQQAALFGQMAFEPGMIKNTYGTGSFIVMNTGEKPHISKNNMLTTIGYGINGKVY
CCCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEE
YALEGSIFVAGSSIQWLRDGLKMLERASDSEEVALQSRSQDEVYVVPAFVGLGAPYWDQE
EEEECEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEHHHCCCCCCCCC
ARGAMFGLTRGTTKEDIVKATLQGIAYQVRDVVSTMKEDTGIDMSVLKVDGGAANNEYLM
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHE
QFQADILNIPIQRAGDLETTALGAAFLAGLAVGFWKDLEELKESYEPGKIFQTQMQEERR
EEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EELYAGWKKAVNATRAFK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TETSYIMAIDQGTTSSRAIIFDKHGKHIGSSQKEFTQYFPKEGWVEHDANEIWNSVQSV
CCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESGIKPVQVAGIGITNQRETTIIWDKKTGKPIYHAIVWQSRQSADIANGLKEAG
HHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCC
HEELFHKKTGLIVDSYFSATKIRWILDHVEGAQERAERGELLFGTIDTWLLWKLTDGDAH
HHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEEEEEEECCCCC
YTDYSNASRTMLYNIHELKWDEEILKILNIPASMLPEVKSNSEVYGVTKGFHFFGSEVPI
CCCCCCCCCEEEEEHHHHCHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCHHHCCCCCCC
SGMAGDQQAALFGQMAFEPGMIKNTYGTGSFIVMNTGEKPHISKNNMLTTIGYGINGKVY
CCCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEE
YALEGSIFVAGSSIQWLRDGLKMLERASDSEEVALQSRSQDEVYVVPAFVGLGAPYWDQE
EEEECEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEHHHCCCCCCCCC
ARGAMFGLTRGTTKEDIVKATLQGIAYQVRDVVSTMKEDTGIDMSVLKVDGGAANNEYLM
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHE
QFQADILNIPIQRAGDLETTALGAAFLAGLAVGFWKDLEELKESYEPGKIFQTQMQEERR
EEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EELYAGWKKAVNATRAFK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471