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The map label for this gene is ykiF

Identifier: 15673057

GI number: 15673057

Start: 1086489

End: 1087304

Strand: Reverse

Name: ykiF

Synonym: L86251

Alternate gene names: 15673057

Gene position: 1087304-1086489 (Counterclockwise)

Preceding gene: 15673058

Following gene: 15673056

Centisome position: 45.96

GC content: 37.62

Gene sequence:

>816_bases
ATGATTAAACTTGTTGCTATTGACCTTGACGGAACTTTACTTGACCCTAATCGCCAAATTACTGCCGAAGTAAAAACGGC
TGTTAAAAAAGCTAAAGCGGCTGGCGTTAAAATTGTCATTACAACTGGCCGACCTTTACCTGGCGTTGTTGATATTCTAA
AAGCACTTGAATTAACTGACCAAAGTGATTATGTTATTACTTATAATGGTGGGTTGGTTCAGCGAGCAACTGGTGAAGAA
TTTATCAAAGAAACTTTGAGTTCTGAAGATTGGCTTGATTTAGATGCTGCTGCTCGTAAAATTGGACTGCCGATTCACGC
AATCACTCGCGAGGGAATCTATACTCCAAATCACGATGTCGGAAGATATACTGTCCAAGAAGCCCAAATGGTTAAAATGC
CACTTTATATTCGCCAACCCGAAGATATTGCTGCTTTAGAAATTGCTAAAGTAATGATGGTTGATGAACCGGCGGCTCTT
GATGACGGAATTGCATACCTTCCCTTTGAATTCTTTGAACGATATAATGTAGTGAAATCAACACCGTTTTATTTAGAATT
CATGAATAAAAAAGCAAGTAAAGGGTCAGCAGTTCAACATTTGGCTGAGAAACTTTCTTTTGACTTGGATGAAGTAATGG
CTATTGGAGATGAAGAAAATGACCGTTCAATGCTTGAAGTTGCAGGTTGTCCTGTTGTGATGGAAAACGGAAAATCGGAA
TTGAAAAAAATTGCTAAATATGTAACTAAATCAAATGCTAAATCAGGCGTTGCTTATGCGATTAATGAATGGGTTTTAAA
AGATTACCAAGATTAA

Upstream 100 bases:

>100_bases
GAAAATACTGACGACTCTGTCAGTAAACAATTATCACTTGATGACAACCTATAATTGCTAAATATTTAATAAATTGAAAG
AAATAGAAAGAGAAATTCTT

Downstream 100 bases:

>100_bases
TTAAAAATTTATGAGCATTATTGTTCCCTACTGAACTTTTTAGCGTTATAATATAAGTATAAAAAGATAACAAATAGATG
AATTTACAAGTCATCTCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MIKLVAIDLDGTLLDPNRQITAEVKTAVKKAKAAGVKIVITTGRPLPGVVDILKALELTDQSDYVITYNGGLVQRATGEE
FIKETLSSEDWLDLDAAARKIGLPIHAITREGIYTPNHDVGRYTVQEAQMVKMPLYIRQPEDIAALEIAKVMMVDEPAAL
DDGIAYLPFEFFERYNVVKSTPFYLEFMNKKASKGSAVQHLAEKLSFDLDEVMAIGDEENDRSMLEVAGCPVVMENGKSE
LKKIAKYVTKSNAKSGVAYAINEWVLKDYQD

Sequences:

>Translated_271_residues
MIKLVAIDLDGTLLDPNRQITAEVKTAVKKAKAAGVKIVITTGRPLPGVVDILKALELTDQSDYVITYNGGLVQRATGEE
FIKETLSSEDWLDLDAAARKIGLPIHAITREGIYTPNHDVGRYTVQEAQMVKMPLYIRQPEDIAALEIAKVMMVDEPAAL
DDGIAYLPFEFFERYNVVKSTPFYLEFMNKKASKGSAVQHLAEKLSFDLDEVMAIGDEENDRSMLEVAGCPVVMENGKSE
LKKIAKYVTKSNAKSGVAYAINEWVLKDYQD
>Mature_271_residues
MIKLVAIDLDGTLLDPNRQITAEVKTAVKKAKAAGVKIVITTGRPLPGVVDILKALELTDQSDYVITYNGGLVQRATGEE
FIKETLSSEDWLDLDAAARKIGLPIHAITREGIYTPNHDVGRYTVQEAQMVKMPLYIRQPEDIAALEIAKVMMVDEPAAL
DDGIAYLPFEFFERYNVVKSTPFYLEFMNKKASKGSAVQHLAEKLSFDLDEVMAIGDEENDRSMLEVAGCPVVMENGKSE
LKKIAKYVTKSNAKSGVAYAINEWVLKDYQD

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=268, Percent_Identity=45.5223880597015, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI48994981, Length=278, Percent_Identity=28.4172661870504, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081741, Length=275, Percent_Identity=25.8181818181818, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30049; Mature: 30049

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLVAIDLDGTLLDPNRQITAEVKTAVKKAKAAGVKIVITTGRPLPGVVDILKALELTD
CEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCC
QSDYVITYNGGLVQRATGEEFIKETLSSEDWLDLDAAARKIGLPIHAITREGIYTPNHDV
CCCEEEEECCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCC
GRYTVQEAQMVKMPLYIRQPEDIAALEIAKVMMVDEPAALDDGIAYLPFEFFERYNVVKS
CCEEHHHHHHEECCEEEECCCCHHHHHHHHHHEECCCCCCCCCCEECCHHHHHHHCHHCC
TPFYLEFMNKKASKGSAVQHLAEKLSFDLDEVMAIGDEENDRSMLEVAGCPVVMENGKSE
CCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHCCCCEEEECCHHH
LKKIAKYVTKSNAKSGVAYAINEWVLKDYQD
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLVAIDLDGTLLDPNRQITAEVKTAVKKAKAAGVKIVITTGRPLPGVVDILKALELTD
CEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCC
QSDYVITYNGGLVQRATGEEFIKETLSSEDWLDLDAAARKIGLPIHAITREGIYTPNHDV
CCCEEEEECCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCC
GRYTVQEAQMVKMPLYIRQPEDIAALEIAKVMMVDEPAALDDGIAYLPFEFFERYNVVKS
CCEEHHHHHHEECCEEEECCCCHHHHHHHHHHEECCCCCCCCCCEECCHHHHHHHCHHCC
TPFYLEFMNKKASKGSAVQHLAEKLSFDLDEVMAIGDEENDRSMLEVAGCPVVMENGKSE
CCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHCCCCEEEECCHHH
LKKIAKYVTKSNAKSGVAYAINEWVLKDYQD
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA